1. The generic Genome Browser (GBrowse) A combination database and interactive web page for manipulating and displaying annotations on genomes Developed by GMOD (Generic Model Organism Database) Citrus GBrowse : a customized GBrowse for the Citrus genome 1 Citrus GBrowse

2. Citrus GBrowse Overview Description menus Search Bird’s eye view Details Tracks 2

3. Citrus GBrowse Search Region displayed Search by annotation ID, name, or comment Hide Details Open Details Dropdown list Scroll/zoom ( plus-sign ) ( minus-sign ) 3

4. Citrus GBrowse Search 4 click

5. Citrus GBrowse Tracks 5 remove track (cross-sign)

6. Citrus GBrowse Tracks 6 choose tracks you want to see

7. Citrus GBrowse Tracks 7 click

8. Citrus GBrowse Tracks 8 Selecting regions with mouse

9. Citrus GBrowse Tracks 9 click

10. Citrus GBrowse Configuring Tracks click 10 Customize your track views

11. Citrus GBrowse Preferences click “Image Width”

12. Citrus GBrowse Preferences 12 Highlights the specified region and feature click edit

13. Citrus GBrowse Reports 13 Download sequences or track data Display settings clic k

14. Citrus GBrowse Reports 14

15. Citrus GBrowse – Adding your annotations 15 Upload or import your own annotation files

16. References http://www.openhelix.com/gbrowse http://gmod.org/wiki/Gbrowse Acknowledgements This tutorial was based on the Open Helix GBrowse 1.6 tutorial which was modified for the Citrus genome sequence and annotations implemented in GBrowse 2.0 16 Citrus GBrowse Tutorial