1. Cell Membranes!  OOOOooooo….  Fluid Mosaic!

2. Membrane structure, I  Selective permeability  Amphipathic~ hydrophobic & hydrophilic regions  Singer-Nicolson: fluid mosaic model

3. Membrane structure, II  Phospholipids~ membrane fluidity  Cholesterol~ membrane stabilization  “Mosaic” Structure~  Integral proteins~ transmembrane proteins  Peripheral proteins~ surface of membrane  Membrane carbohydrates ~ cell to cell recognition; oligosaccharides (cell markers); glycolipids; glycoproteins Animation

4. Membrane structure, III  Membrane protein function:  transport  enzymatic activity  signal transduction  intercellular joining  cell-cell recognition  ECM attachment

5. Membrane traffic  Diffusion~ tendency of molecules to move from a high to low concentration by random movement.  Solutes will diffuse down their own concentration gradients. l NOT affected by concentration gradients of other solutes!!!  Osmosis~ the diffusion of water across a selectively permeable membrane.  Passive transport~ diffusion of a substance across a biological membrane. l Does NOT require energy l Goes DOWN a concentration gradient  Active Transport ~ movement across a membrane that DOES require energy. l Usually goes UP a concentration gradient. l Energy, of course, usually supplied by _____ ATP! Animation

6. Water balance  Osmoregulation~ control of water balance  When comparing 2 sides of a membrane, one side may be:  Hypertonic~ higher solute concentration ( and the other side will be…)  Hypotonic~ lower solute concentration  Water will move to the Hypertonic!! l Think of it as “watering it down”.  Isotonic~ equal concentrations of solutes  Cells with Walls:  Turgid (very firm)  Flaccid (limp)  Plasmolysis~ plasma membrane pulls away from cell wall

7. Specialized Transport  Transport proteins:  Facilitated diffusion~ passage of molecules and ions with transport proteins across a membrane down the concentration gradient  Active transport~ movement of a substance against its concentration gradient with the help of cellular energy Animation

8. Types of Active Transport  Sodium-potassium pump  Exocytosis~ secretion of macromolecules by the fusion of vesicles with the plasma membrane  Endocytosis~ import of macromolecules by forming new vesicles with the plasma membrane l 3 types of Endocytosis:  phagocytosis- “cell eating”  pinocytosis- “cell drinking”  receptor-mediated endocytosis Animation WebLink