1. rh Lecture 7 Slides

2. Great new Krebs cycle (Citric Acid cycle) movie… fig 16-13 http://www.youtube.com/watch?v=A1DjTM1qnPM

3. Krebs cycle features and functions… fig 16-13

4. CH 3 -C O O-O- O acetate acetyl group S-CoA AcCoA acetyl coenzyme A or acetyl-CoA CH 3 -C O it’s all about acetate… rh

5. fig16-1 all roads lead to acetyl-CoA…

6. fig16-1 electrons from acetyl groups

7. fig16-1 energy from electrons

8. fig16-3 This is HS-CoA, or CoA-SH, CoA, or coenzyme A

9. fig16-3 This is HS-CoA, or CoA-SH, CoA, or coenzyme A CoA-SH, or HS-CoA, or CoA

10. fig16-2 where the acetyl groups come from pyruvate dehydrogenase: PDH

11. fig 14-13 Thiamine pyrophosphate (TPP): an activated carbon

12. fig 14-13 TPP as a carbanion nucleophile in pyruvate metabolism

13. fig16-4 The “business end” of PDH Lipoic acid cofactor of PDH E 2

14. fig16-5 The PDH complex approx. 4.5 x 10 6 MW

15. fig16-5 The PDH complex

16. fig16-6 (old) The PDH catalytic cycle oxidation of pyruvate

17. Several reactions, one basic location fig16-6

18. Please read and LEARN “The Name Game”

19. fig16-7 The: Krebs cycle citric acid cycle TCA cycle

20. fig 16-8 Citrate synthase and its substrates…

21. pg 622 Getting fresh carbons into the act why it’s called the citric acid cycle

22. pg 622 making citrate asymmetric remember this for later…

23. fig16-10 an iron-sulfer cluster in aconitase

24. pg 624 (less fancy picture) electrons! NADH is the main carrier of Krebs cycle-derived electrons

25. pg 625 more electrons! note CoA-SH has re-entered the picture

26. pg 626 direct production of a high energy phosphate but not ATP… instead it’s GTP

27. pg 626 direct production of a high energy phosphate but not ATP… instead it’s GTP

28. pg 628 electrons to FAD instead of NAD +

29. rh The business end of FAD or FMN isoalloxazine ring

30. pg 628 electrons to FAD instead of NAD +

31. pg 628 a sensible inhibitor of succinate dehydrogenase INHIBITOR

32. old pg 612 Hydration of fumarate

33. pg 628 Hydration of fumarate

34. pg 628 high specificity exemplified by substrates and products of fumarase substrate product not a substrate

35. pg 628 high specificity exemplified by substrates and products of fumarase not a product substrate product

36. pg 628 the final electron removal

37. Krebs cycle features and functions… fig 16-13