36Phorid Fly ReleasesBetween 1996 and 2002 the decapitating fly, Pseudacteon tricuspis, was released at 56 sites in the southeastern United StatesMost releases have been made by the USDA-ARS, CMAVE, Gainesville, FL ( 32 sites) and the University of Texas, Austin (15 sites).
37Phorid Fly ReleasesOverwintering populations of flies were successfully established at 19 sites in 6 states (AL-3, FL- 5, LA-3, MS-1, SC-2, TX-5).
39Thelohania solenopsae, a microsporidian intracellular parasite The most common fire ant pathogen in BrazilIt was discovered in the US in 1998 (FL, TX, MS, OK).Decreases ant’s longevity and reduces colony size
48Volumetric flow rate (Q), the volume of fluid which passes through a given volume per unit time Q = A x v, whereA = area (depth and width of water stream)v = uniform average velocity of water (how long it takes to flow a known distance)Q*time - gives us an estimate of total water runoff
49Sample processingOne liter sample collected from one bank of sprinklers per houseSample stored at 40CSample analyzed at laboratory of Dr. Jay Gan in environmental sciences, UC Riverside
50Schedule 2007Pretreatment ant evaluations and water samples collected in JunePost-treatment ant evaluations done at 1,2,4,and 8 weeksPost-treatment water samples collected at 1, 4, and 8 weeks
51Concentration of fipronil and its byproducts in runoff desulfinylsulfidesulfone
52Concentrations of fipronil and bifenthrin in runoff With Ceriodaphnia LD50sFipronil LD50Bifenthrin LD50
532008 6 different pesticide treatments 3 houses sampled per treatment for insecticide runoff = 18 houses sampledSample periodsPretreatment1 day1 week2 week driveway flush
54This summer’s fipronil treatments All fipronil treatments limited to 1 gal1 gal peripheral spray -- pin stream nozzleSpot treatmentsSame as above but not within 15 ft of street and 5 ft of sidewalks
55This summer’s bifenthrin treatments All use 3 galsPin stream nozzle perimeter treatmentTraditional fan nozzle perimeter applicationSpot treatments with 3 gals, but not within 15 ft of street or 5 ft of sidewalks
56ConclusionsWe can successfully monitor how much insecticide runs off from treated houses due to irrigationWe can test different application techniques to minimize insecticide runoff