Between 1996 and 2002 the decapitating fly, Pseudacteon tricuspis, was released at 56 sites in the southeastern United States Most releases have been made by the USDA-ARS, CMAVE, Gainesville, FL ( 32 sites) and the University of Texas, Austin (15 sites).
Phorid Fly Releases Overwintering populations of flies were successfully established at 19 sites in 6 states (AL-3, FL- 5, LA-3, MS-1, SC-2, TX- 5).
Thelohania solenopsae, a microsporidian intracellular parasite The most common fire ant pathogen in Brazil It was discovered in the US in 1998 (FL, TX, MS, OK). Decreases ant’s longevity and reduces colony size
Volumetric flow rate (Q), the volume of fluid which passes through a given volume per unit time Q = A x v, where –A = area (depth and width of water stream) –v = uniform average velocity of water (how long it takes to flow a known distance) Q*time - gives us an estimate of total water runoff
Sample processing One liter sample collected from one bank of sprinklers per house Sample stored at 4 0 C Sample analyzed at laboratory of Dr. Jay Gan in environmental sciences, UC Riverside
Schedule 2007 Pretreatment ant evaluations and water samples collected in June Post-treatment ant evaluations done at 1,2,4,and 8 weeks Post-treatment water samples collected at 1, 4, and 8 weeks
fipronildesulfinyl sulfide Concentration of fipronil and its byproducts in runoff sulfone
Concentrations of fipronil and bifenthrin in runoff With Ceriodaphnia LD50s Fipronil LD50 Bifenthrin LD50
2008 6 different pesticide treatments –3 houses sampled per treatment for insecticide runoff = 18 houses sampled –Sample periods Pretreatment 1 day 1 week 2 week driveway flush
This summer’s fipronil treatments All fipronil treatments limited to 1 gal –1 gal peripheral spray -- pin stream nozzle –Spot treatments –Same as above but not within 15 ft of street and 5 ft of sidewalks
This summer’s bifenthrin treatments All use 3 gals –Pin stream nozzle perimeter treatment –Traditional fan nozzle perimeter application –Spot treatments with 3 gals, but not within 15 ft of street or 5 ft of sidewalks
Conclusions We can successfully monitor how much insecticide runs off from treated houses due to irrigation We can test different application techniques to minimize insecticide runoff