1. Logistical Considerations for Participation in the PSI-MR John G. Primm, Craig A. Bingman, Gary Wesenberg, Xiaokang Pan, Russell L. Wrobel, Kory D. Seder, Brian G. Fox, George N. Phillips, Jr., and John L. Markley

2. Scope of CESG individual activity Current administrative process for CESG material distribution / Stakeholders New infrastructure developed for participation in PSI-MR Cost / Benefit analysis Considerations and Going Forward

4. Our most popular request…

6. CESG Materials Distribution PSI-2

7. Current Processes Stakeholders

25. StepActionCost Units Involved 1Incoming request for material sent from Requestor to PI or comes in through CESG web site.$26.89PI 2Request is routed to PM office - preliminary review.$15.30PI / PM 3If the material can be shared, material location is extracted from LIMS and verified.$13.24Cloning / PM 4 Project Management office (PM) sets up files and contacts requestor to establishing legal signing entity for their institution.$4.64 PM office / Requestor 5 Upon receipt of Legal information, a boiler plate MTA is form is filled out and UW routing forms are constructed; routing for execution begins.$21.02 PM assistant / PM / PI 6The PI reviews and signs the documents.$15.30PM / PI 7Documents routed to UW Biochemistry chair for review / signature. Copies made and filed.$15.30PM / Biochem 8 Documents routed to UW College of Agriculture and Life Sciences (CALS) for review / signature. Entered into CALS database. Copies filed.$20.09 PM assistant / CALS intake /CALS 9 Wisconsin Alumni Research Foundation (WARF) may be brought in depending on materials / issues.$3.09CALS / WARF 10 After CALS / WARF review, documents are routed to UW Research and Sponsored Programs Office (RSP). MTA entered into RSP database. MTA reviewed and signed. Copies made and filed.$13.91RSP intake / RSP 11RSP routes to legal signatory for institutional signature.$28.91RSP intake / RSP 12Legal signatory routes to recipient scientist for external Requestor signature.$0.00 Tech Transfer Office / Requestor 13Executed copy returned to RSP, originals filed, RSP database status marked as "completed“.$10.43 Tech Transfer Office / RSP 14PM office notifies cloning; cloning pulls and prepares material.$54.94Cloning / PM 15Materials pass through QA step.$84.41Cloning 16Material is packed and sent to Requestor.$32.65Cloning / PM 17Specific information regarding the sample is sent to the Requestor.$13.24Cloning 18 Follow-up questions from the Requestor are directed to and answered by the appropriate CESG group.$26.47 Requestor / Cloning / PM Total Cost for Average MTA and Transfer$399.80

26. Stakeholder Issues Intellectual Property > UW licenses thousands of products now in commerce Liability Tracking of materials deposited Tracking of materials distributed Tracking of materials received Organization and visibility of processes Permanent storage of records Distribution efforts Physical / extraction of manipulation of data

27. Image from photobucket.com Nuts and Bolts

28. Data First ! Plates last!

29. Clone Information Files (Required): Clone Location File Clone Information File Clone Gene Info File

30. Sesame actions required for internal tracking

31. Sesame search results

38. Human Eyes 2 hrs/WG

44. Permanent data storage

45. PSI-MR CESG Quarterly Report Order IDOrder DateStatus Clone ID 987 FEB-06-2008 10:37:40 ShippedAtCD00082681 983 FEB-04-2008 16:36:05 ShippedAtCD00082683 Species Specific ID User At1g70910David Aceti At4g16160David Aceti Shipping Information: CESG-QA 445 Henry Mall Madison, WI 53706 Phone: 608-890-0491 User Email: acetidav@nmrfam.wisc.eduacetidav@nmrfam.wisc.edu PI Name MARKLEY, John PI Institution Univ. Wisconsin-Madison PI Department Biochemistry PI Email markley@nmrfam.wisc.edumarkley@nmrfam.wisc.edu

46. Results to date

51. Of the sample: CESG was able to positively identify 83 % of those mismatched. Pathway for remediation is under discussion.

52. Next Goals Reduce mismatched error rate 13.7% > by remediation to 7.0% Deposit all “declared available” destination clones by Oct 1. Or, better, before any changes in the data format occurs. Improve CESG processes to include multiple vectors clones

53. UnitEstimated time HRS / 500 destination clones Estimated cost / 500 destination clones Projected costs - all remaining destination clones Projected costs - all remaining entry clones Bioinformatics14$711$7,113$1,423 Cloning8$424$4,235$5,929 QA8$349$3,486$697 CESG Admin0.5$31$306$428 RSP0.1$4$42$58 CALS0.1$5$48$67 Totals MR deposition30.7$1,523$15,231$8,604 Totals for traditional distribution$45,885 Projected Savings$22,051

54. Costs of Implementation UnitEstimated time HRSInvestment Bioinformatics280$15,105.35 Cloning80$4,235.28 QA20$871.53 CESG Admin45$4,158.33 RSP10$417.22 CALS18$867.83

55. Cost of MR Participation Summary Infrastructure Investment $24,700 Projected Savings($22,000) Priceless!

56. Looking Forward PSI-MR will receive significant attention as useful biological research materials for biomedically relevant proteins whose purification properties and structures are known become available. Each Center preparing TargetDB files should already have accumulated all or nearly all of the information needed to prepare clone submission files for the PSI-MR. Opportunities are now emerging to create a wonderful synergy between the PSI-MR, PepcDB, and the PSI Knowledgebase. –require that a unified, searchable tag is seeded into all three databases

57. Opportunity Risk Requestors need and expect all information required (Protocols) to replicate your success.

60. Data Preparation Depositors Agreement

61. Data Preparation Depositors Agreement

62. Data Preparation Depositors Agreement

63. Expedited MTA Covers Entire Institutions Data Preparation Depositors Agreement

64. Expedited MTA Covers Entire Institutions Data Preparation Depositors Agreement

65. Expedited MTA Covers Entire Institutions Data Preparation Depositors Agreement

66. Expedited MTA Covers Entire Institutions Data Preparation Depositors Agreement

67. Dancing in the Streets

68. Acknowledgements CESG gratefully acknowledges the collaborative interactions with the following personnel of the PSI-MR as an essential part of this effort: Catherine Cormier, Joshua LaBaer, Stephanie Mohr, Andreas Rolfs, Elena Taycher, and Dongmei Zuo. We also thank all members of CESG, including Arash Bahrami, Lenka Bittova, Lai Bergeman, E. Sethe Bergie, Christopher Bianchetti, Eduard Bitto, Claudia Cornilescu, Ronnie Frederick, Michael Goren, Katarzyna Gromek, Leigh Grundhoefer, Teagan Hayes, Andrew Larkin, Elena Levin, Betsy Lytle, Shin-Ichi Makino, John Markley, Yuko Matsubara, Karl Nichols, Xiaokang Pan, Francis Peterson, Mike Popelars, Sarata Sahu, Kory Seder, Donna Troestler, Frank Vojtik, Brian Volkman, Gary Wesenberg, Russell Wrobel, and Zsolt Zolnai for their support of this important initiative.