1. By: Cassie Silvanima & Krista Cunningham Rolled leaf1 (Rld1), CAS, KEC, 1-13-05

2. Basic Information Protein sequence Growth chart Grown up corn Explanation

3. Rld1 is a dominant trait, it causes the plants leaves to never fully unroll. The genetic mutation is on chromosome 9. It is not a lethal mutation. The Rld1 gene has been cloned.

4. GenBank Accession AAR97952 rolled leaf1 [Zea mays] protein (amino acid) sequence: 1 MVAAVALRGG SSDSGGFDKV PGMDSGKYVR YTPEQVEVLE RLYIDCPKPS 51 SSRRQQLLRE CPILSNIEPK QIKVWFQNRR CRDKQRKESS RLQAVNRKLT 101 AMNKLLMEEN ERLQKQVSQL VHENAHMRQQ LQNTSLANDT SCESNVTTPP 151 NPIRDASNPS GLLAIAEETF TEFLSKATGT AIDWVQMPGM KPGPDSVGIV 201 AISHGCRGVA ARACGLVNLE PTKGIEILKD RPSWFRDCRS LEVFTRFPAG 251 NGGTIELIYM QMYAPTTLVP ARDFWTLRYT TTMEDGSLVV CERSLSGSGG 301 GPNAASTQQF VRAEMLPSGY LVRPCEGGGS IVHIVDHLDL EAWSVPEVLR 351 PLYESSRVVA QKMTTVALRH LRQIAQETSG EVVYALGRQP AVLRTFSQRL 401 SRGFNDAISG FNDDGWSVMG GDGIEDVVIA CNSTKKIRNT SNAGITFGAP 451 GGIICAKASM LLQSVPPAVL VRFLREHRSE WADYNIDAYL ASSLKTSACS 501 LPGLRPMRFS EGQMIMPLAH TVENEEILEV VRLEGQPLTH DEALLSRDIH 551 LLQLCTGIDE KSVGSSFQLV FAPIDEHFPD DAPLISSGFR VIPLDVKTDG 601 VSSGRTLDLA SSLDVGSAAP QASGDASPDD CSLRSVLTIA FQFPYEMHLQ 651 DSVAAMARQY VRSVISAVQR VSMAISPSQS GLNAGHRMLS GFPEAATLAR 701 WVCQSYHYHL GMELLNQSDG AGEALLKMLW HHPDAVLCCS FKEKPMFTFA 751 NKAGLDMLET SLVALQDLTL DKIFDESGRK ALFSDISKLM EQGYAYLPSG 801 VCMSGMGRHV SFDQAVAWKV LGEDSNIHCL AFCFVNWSFV

5. Date Mean (cm) Leaf Other Height, color width notes 9/10 [planting date] 9/17 7.64, light green 0.66 big seedlings 9/24 26.8, light green 1.20 not much roll 10/01 30.7, dark green 1.10 purple stems gone

6. Now that our corn grew up Mr. Bass demonstrated to the class how to cross corn so that we could make more mutant seeds. We didn’t expect our corn to have silks and tassels because we grew them in the middle of winter, but they did!

7. This Punnett square shows how corn reproduces. This is a double helix. A a A AA Aa a aA aa mother father children