Presentation is loading. Please wait.

Presentation is loading. Please wait.

EAEC51 Study start 8-13-2013 N or NZD fed 14d/ab pretreatment/10^9 EAEC 042 EAEC51 Study Design Objectives/Questions Repeat EAEC40 with nourished control.

Published byMary Watkins Modified over 8 years ago

Similar presentations

Presentation on theme: "EAEC51 Study start 8-13-2013 N or NZD fed 14d/ab pretreatment/10^9 EAEC 042 EAEC51 Study Design Objectives/Questions Repeat EAEC40 with nourished control."— Presentation transcript:

1 EAEC51 Study start 8-13-2013 N or NZD fed 14d/ab pretreatment/10^9 EAEC 042 EAEC51 Study Design Objectives/Questions Repeat EAEC40 with nourished control diet made with egg white protein Generate additional data for Bolick, et al. 2013 manuscript Day mice delivered -3 Tues -14 Tues Design 1)C57Bl/6 male mice 4 weeks old on arrival 2)Nourished (N) or Nourished-Zinc (NZD) diet fed for two weeks 3) Antibiotic cocktail pretreatment for 2 days, followed by 1 day off 4) weights, stools collected every day, sac day 3 EAEC 10 9 0 weights and stools Diet Start Thurs ab Euthanasia for collection 3 Zinc depletion

2

3

4

5 To do for EAEC 51 Mx Histology (done, needs pics and IHC for CD45) qPCR for cytokines qPCR for in vivo virulence factor expression Final revisions and where to submit

6 IL-17r Study

7

8 IL-17rKO To Do Histology (done, needs pics and IHC?) qPCR (Th17 pathway plate) Tissue burden (done, needs graph) Mice are in, MTA is still in approval process IL-25?

Download ppt "EAEC51 Study start 8-13-2013 N or NZD fed 14d/ab pretreatment/10^9 EAEC 042 EAEC51 Study Design Objectives/Questions Repeat EAEC40 with nourished control."

Similar presentations

The FATZO mouse as a Translational Model for the Development of Drugs for Obesity, Metabolic Syndrome and Diabetes. PreClinOmics, Inc. 1.

The FATZO mouse as a Translational Model for the Development of Drugs for Obesity, Metabolic Syndrome and Diabetes. PreClinOmics, Inc. 1.
Supplementary methods WST-1 assay of cell viability/proliferation. Raw264.7 or πBCL1 cells cultured with liposomes encapsulating dichloromethylene-bisphosphonate.

Supplementary methods WST-1 assay of cell viability/proliferation. Raw264.7 or πBCL1 cells cultured with liposomes encapsulating dichloromethylene-bisphosphonate.
Figure S1. Zhao et al. a b c Figure. S1. VEGF does not stimulate population increase and cell cycle changes in breast cancer cells. (a) 20000 cells from.

Figure S1. Zhao et al. a b c Figure. S1. VEGF does not stimulate population increase and cell cycle changes in breast cancer cells. (a) cells from.
Anti-CD40 and CpG induce activation of T cells in draining lymph nodes

Anti-CD40 and CpG induce activation of T cells in draining lymph nodes
Mice deficient in the NF-κB negative regulator, itch, develop severe osteoarthritis and reduced synovial lymphatic drainage due to m1 macrophages-induced.

Mice deficient in the NF-κB negative regulator, itch, develop severe osteoarthritis and reduced synovial lymphatic drainage due to m1 macrophages-induced.
Volume 16, Issue 2, Pages (July 2016)

Volume 16, Issue 2, Pages (July 2016)
Partial knockdown of MPST markedly ameliorates hepatic steatosis in vivo. Partial knockdown of MPST markedly ameliorates hepatic steatosis in vivo. (A–C)

Partial knockdown of MPST markedly ameliorates hepatic steatosis in vivo. Partial knockdown of MPST markedly ameliorates hepatic steatosis in vivo. (A–C)
Fig. 4. NASH-driven fibrosis is partly TGF-β–dependent.

Fig. 4. NASH-driven fibrosis is partly TGF-β–dependent.
Volume 22, Issue 1, Pages (July 2015)

Volume 22, Issue 1, Pages (July 2015)
Mean and difference Raman spectra of spleen tissue from endotoxemic mice and sham mice and PCA-LDA. Mean and difference Raman spectra of spleen tissue.

Mean and difference Raman spectra of spleen tissue from endotoxemic mice and sham mice and PCA-LDA. Mean and difference Raman spectra of spleen tissue.
Lung interleukin (IL)-33 protein expression in vivo depends on caspase-1. Lung interleukin (IL)-33 protein expression in vivo depends on caspase-1. Mice.

Lung interleukin (IL)-33 protein expression in vivo depends on caspase-1. Lung interleukin (IL)-33 protein expression in vivo depends on caspase-1. Mice.
Type 1 immunity drives metabolic disease but protects against NAFLD

Type 1 immunity drives metabolic disease but protects against NAFLD
Enhanced proliferation and cytokine production in DGKζ and Cbl-b deficient mice. Enhanced proliferation and cytokine production in DGKζ and Cbl-b deficient.

Enhanced proliferation and cytokine production in DGKζ and Cbl-b deficient mice. Enhanced proliferation and cytokine production in DGKζ and Cbl-b deficient.
Fig. 5. Accelerated NASH-driven fibrogenesis in obese IFN-γ−/− mice is characterized by severe eosinophilic inflammation during TGF-β blockade. Accelerated.

Fig. 5. Accelerated NASH-driven fibrogenesis in obese IFN-γ−/− mice is characterized by severe eosinophilic inflammation during TGF-β blockade. Accelerated.
Effect of plumbagin (PL) on the growth of prostate tumors in Pten-KO mice. Effect of plumbagin (PL) on the growth of prostate tumors in Pten-KO mice. A.

Effect of plumbagin (PL) on the growth of prostate tumors in Pten-KO mice. Effect of plumbagin (PL) on the growth of prostate tumors in Pten-KO mice. A.
Volume 23, Issue 4, Pages (April 2016)

Volume 23, Issue 4, Pages (April 2016)
Viperin−/− mice have high levels of pro-inflammatory cytokines but low levels of Th2 cytokines. Viperin−/− mice have high levels of pro-inflammatory cytokines.

Viperin−/− mice have high levels of pro-inflammatory cytokines but low levels of Th2 cytokines. Viperin−/− mice have high levels of pro-inflammatory cytokines.
Myung H. Kim, Elizabeth J. Taparowsky, Chang H. Kim  Immunity 

Myung H. Kim, Elizabeth J. Taparowsky, Chang H. Kim  Immunity 
T cell–intrinsic STING inhibits cell cycle progression of T cells.

T cell–intrinsic STING inhibits cell cycle progression of T cells.
The relationship between the genes in mice and human samples

The relationship between the genes in mice and human samples

Similar presentations

Ads by Google