1. To Do Electron Microscopy Lecture
Results from Environmental Plates & Broths
Serial Dilution & Streak Plates
Fungi: make wetmounts & observe demo’s
Observe gram stain demo’s/finish old slides

2. Relative Size of objects

3. Resolution Analogy

4. Resolution

5. Anatomy of a Wave

6. Properties of Light
Wavelength: Length of a light ray; represented by the Greek letter lambda ()
Equal to the distance between two adjacent crests or troughs of a wave
Resolution: Refers to the ability to see two items as separate and discrete units

7. Properties of Light
Resolving Power (RP): minimal distance two points can be distinguished as two points
The smaller the distance between objects that can be distinguished, the greater the resolving power of the lens
RP= /2(NA)

8. Microscope Comparison

9. Modern Scanning Electron Microscope

10. Limits of Resolution Eye: 0.2 mm = 200 microns = 200,000 nm
BF scope: mm =0.2 microns = 200 nm
TEM: mm = 0.01 microns = 10 nm
Fig. 3.p039

11. E . coli Brightfield KM

12. Comparison of Electron Microscopes
E.coli SEM
E. coli TEM

13. Resolution vs. Magnification
Light Microscope
SEM

14. SEM

15. TEM

16. Type of Scope?

17. Type of Scope?

18. ID Microbe & Specific Scope
CDC, used with permission

19. Microscope comparison John Wiley

20. Results from Environmental Samples
Learn how to interpret broth culture growth patterns

21. Broth comparison
uninoculated

22. Various Colored Colonies

24. Review Properties of Agar
Comes from Red algae
Microbes usually don’t eat it
Not toxic
Melts at 100 C
Solidifies at 40 C

25. Preparation of a pure Culture

26. 4 Quadrant Streak Plate
3. loop
2. loop
4. loop
1. swab
Do right NOW

27. Quantitation of Bacteria

28. Serial Dilutions

29. Comparison of Serial Dilution Techniques

30. Pour Plate

31. Countable number of colonies (20 to 200 per plate)
Which of these plates would be the correct one to count? Why?

32. Serial Dilutions

34. Fungal Demo’s Gram Stain Demo’s
Aspergillus Treponema pallidum
Rhizopus Neisseria gonorrhoeae
Penicillium Corynebacterium
Candida

35. Aspergillus
100x
400x KM

36. What are these? KM
400x

37. Rhizopus
100x KM

38. Penicillium
100x x

39. Candida
100x
400x KM

40. Candida & staphylococciKM

41. Fungal Demo’s Gram Stain Demo’s
Aspergillus Treponema pallidum
Rhizopus Neisseria gonorrhoeae
Penicillium Corynebacterium
Candida

42. Treponema
CDC, used with permission

43. Treponema
1000x KM
Wikipedia Commons, used with permission

44. Diplococci of Neisseria gonorrhoeae (4087X)

45. TEM diplococci of Neisseria gonorrhoeae (96,051X)

46. Neisseria
1000x KM

47. Neisseria
1000x KM

48. Corynebacterium diphtheria
CDC, used with permission

49. Corynebacterium diphtheria—the cause of diphtheria

50. Corynebacterium
1000x KM

51. Fungal Demo’s Gram Stain Demo’s
Aspergillus Treponema pallidum
Rhizopus Neisseria gonorrhoeae
Penicillium Corynebacterium
Candida

55. ID Microbe & Specific Scope