1. Drug Discovery and Development
Hits
Leads
Candidate optimization

2. PHILIPPINE PHARMASEAS DRUG DISCOVERY PROGRAM
Marine Drug Discovery of
Anti-Pain Agents from Turrid Snails
Anti-Infective Agents from Sponge – Associated Microorganisms

3. Philippine Mollusk Symbiont International Biodiversity Group (PMS-ICBG)
Diverse drug lead compounds from bacterial symbionts in tropical marine mollusks
A research collaboration between University of the Philippines Diliman, Oregon Health and Science University, University of Utah, Academy of Natural Sciences of Philadelphia and Ocean Genome Legacy Foundation, USA.

4. Drug Discovery Pipeline1 2
Collection and isolation of microorganisms
High throughput bioactivity screening 5 3
Novel bioactive compounds 4
Chemical extraction/
Dereplication at compound level
Identification of isolates/
Dereplication at producer level

5. Isolation of Microorganisms
2,737
Marine micoorganisms in collection
Field collection of marine sponges, gastropods
From database as of April 2012
Isolation of morphologically distinct colony
Database and archiving of pure isolates

6. Bioactivity Screening
169
Antimicrobial Activity
Pseudomonas aeruginosa (PA)
Klebsiella pneumoniae (KP)
Staphylococcus aureus (SA)
Methicilin-resistant S. aureus (MRSA)
Biofilm-forming S. epidermidis
Mammalian Cell Cytotoxicity
Antiviral Activity
Antifungal Activity
Candida albicans (CA)
Ampothericin-resistant C. albicans
Neuroactivity
Dorsal root ganglion assay DRG
Antimicrobial
priority isolates 78
Cytotoxic
priority isolates
Neuroactive
priority isolates 11
4,872
Yields from assay events

7. Strategy for Finding High Value Compounds: Purifying compounds from Sponge MMO
HPLC Conditions:
Shimadzu Prominence HPLC Injected: 50 μL
Flow rate: 3.0mL/min Solvent system:Binary gradient,
Column: Phenomenex Luna C MeOH in 45 mins
250x10mm, 5μm (semi prep)
100% MeOH Diaion Fraction
6 7 8 9

8. Chemical Dereplication
UV absorbance spectra
Compound matches
Hi-res mass spectra + fragmention data
AntiMarin*
Database
+ bioactivity and
Isolate identity data
NMR data (H-H COSY)
AntiMarin – is a combined database containing 55,000 reported terrestial and marine natural products
Recent tool

9. Materials Science Materials Bioactive compounds and analogues
Nano materials
Immuno- liposomes
Ionic
liquids
Hybrid nano- composites
Nano composites

10. Magnetic Resonance Imaging
Radiologic pharmaceuticals
Polymeric Gd(III)-based macromolecular MRI contrast agents
Ln(III)-labelled secondary metabolite peptides as novel molecular imaging tools of pain receptors
Ln(III) complexes as spectroscopic labels for targeted diagnostic molecular imaging

11. Reach us at . . .

15. NMR User Fees

16. NMR Facility Core Group
Administration: Dr. Armando Somintac (NIP) and Dr. Neil Andrew Bascos (NIMBB)
Promotion and advertisements: Dr. Evangeline C. Amor (IC) and Dr. Christine C. Hernandez (IC)
Training and licensing: Dr. Florecita de Guzman (IC), Dr. Portia Mahal G. Sabido (IC), Dr. Aaron Villaraza (IC), Dr. Nemesio E. Montano (MSI)
Maintenance: Mr. Miguel Azcuna (MSI) and Ms. Arlene Bartolome (IC)
Housekeeping: Dr. Irene M. Villaseñor (IC)
Finance: Dr. Irene M. Villaseñor (IC)
Business: Dr. Gisela P. Concepcion (MSI)