1. 1. 2. 3. 4. 5. 6. © Chemometric Optimization Of A SIA Promethazine Hydrochloride Assay Method Idris, AM; Assubaie, FN; Sultan, SM ELSEVIER SCIENCE BV, MICROCHEMICAL JOURNAL; pp: 7-13; Vol: 83 King Fahd University of Petroleum & Minerals http://www.kfupm.edu.sa Summary A sequential injection analysis (SIA) method for the assay of promethazine hydrochloride, based on its oxidation by acidified cerium(W), was optimized. Three chemometric approaches were applied: (i) factorial design (3(3) applied to surface plot and 2(3) applied to effect factor) for screening the potential interacting variables, (ii) univariant for optimizing insignificantly interacting variables and (iii) simplex for optimizing potentially interacting variables. The optimum experimental conditions were 30 mu l of 0.38 mol/l sulphuric acid, 30 mu l of 3.99 x 10(-3) mol/l cerium(IV), 20 mu l of promethazine hydrochloride and 20 mu l/s flow rate. The detection limit was 7.032 x 10(-5) mol/l and the calibration curve was linear up to 1.563 mol/l with a correlation coefficient 0.9998, accuracy range of 89.0-101.5%, relative standard deviation 1.1 % (n = 10) and sample frequency at least 20 samples/h. The method was applied to tablet form and validated with the British Pharmacopoeia method. The developed SIA method is fully automated, reproducible, sensitive, rapid and reagent- saving, and therefore suitable for routine control in tablets form. (c) 2006 Elsevier B.V All rights reserved. References: *BRIT PHARM, E COP CD VER 6 0 *GRAB SOL, 2000, MULTISIMPLEX US GUID *US PHARM, 2003, E COP CD VER 26 NF 2 ALWARTHAN AA, 1993, ANAL CHIM ACTA, V282, P169 AMAN T, 2003, ANAL LETT, V36, P2961, DOI 10.1081/AL-120026414 BASAVAIAH K, 1998, TALANTA, V47, P59 Copyright: King Fahd University of Petroleum & Minerals; http://www.kfupm.edu.sa

2. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. 19. 20. 21. 22. 23. 24. 25. 26. 27. 28. 29. 30. 31. © BASAVAIAH K, 2000, FARMACO, V55, P87 BASAVAIAH K, 2001, ANAL SCI, V17, P963 BASAVAIAH K, 2001, FARMACO, V56, P579 BASAVAIAH K, 2002, TURK J CHEM, V26, P551 BASAVAIAH K, 2003, INDIAN J CHEM TECHN, V10, P356 BASAVAIAH K, 2004, FARMACO, V59, P315 BAXTER RI, 1984, ANAL CHIM ACTA, V164, P171 CALATAYUD JM, 1992, ANAL CHIM ACTA, V264, P283 CALATAYUD JM, 1992, J PHARMACEUT BIOMED, V10, P37 CALATAYUD JM, 1992, MICROCHEM J, V45, P129 CHEN D, 1991, TALANTA, V38, P1227 DANIEL D, 2003, ANAL CHIM ACTA, V494, P215, DOI 10.1016/S0003-2670(03)00903-6 DEORSI D, 1996, J PHARMACEUT BIOMED, V14, P1635 DIAZ AN, 1991, ANAL CHIM ACTA, V255, P297 FARHADI K, 2003, ACTA CHIM SLOV, V50, P395 FEHER Z, 1988, ANAL BIOMED CHEM, V32, P345 GOLABI SM, 1991, TALANTA, V38, P1253 GOWDA HS, 1978, ANAL CHIM ACTA, V99, P343 HOMYAK I, 1997, BIOMED CHROMATOGR, V11, P99 ISSA AS, 1984, TALANTA, V31, P287 ISSA YM, 2000, MIKROCHIM ACTA, V134, P9 KERDWAY MM, 1992, MIKROCHIM ACTA, V108, P323 KUZMICKA L, 1988, PHARMAZIE, V43, P288 LARA FJ, 2005, ANAL CHIM ACTA, V535, P101, DOI 10.1016/j.aca.2004.11.081 32. LIMA JLFC, 1997, J PHARM SCI, V86, P1234 33. MISIUK W, 1996, PHARMAZIE, V51, P62 34. MOHAMED FA, 1995, ANAL LETT, V28, P2491 35. MUIJSELAAR PGHM, 1996, J CHROMATOGR A, V735, P395 36. NAGARAJA P, 2000, ANAL SCI, V16, P1127 37. NI YN, 2001, ANAL CHIM ACTA, V439, P159 38. PENA L, 1993, J PHARM BIOMED ANAL, V11, P893 39. PUZANOWSKATARAS.H, 1989, PHARMAZIE, V44, P35 40. RAMA MJR, 2004, J PHARMACEUT BIOMED, V35, P1027, DOI 41. 10.1016/j/jphs.2004.03.010 42. REGULSKA E, 2002, J PHARMACEUT BIOMED, V27, P335 43. ROMERO AM, 1992, ANAL LETT, V7, P1289 44. SAIF MJ, 2005, TALANTA, V67, P869, DOI 10.1016/j.talanta.2005.03.034 45. SHAMSIPUR M, 2002, J AOAC INT, V85, P555 46. SULTAN SM, 1993, TALANTA, V40, P681 47. SULTAN SM, 1995, ANALYST, V120, P561 48. SULTAN SM, 2003, TALANTA, V59, P1073, DOI 10.1016/S0039- 9140(03)00016-X 49. TAHA AM, 1983, ANALYST, V108, P1500 Copyright: King Fahd University of Petroleum & Minerals; http://www.kfupm.edu.sa

3. 50. 51. 52. 53. 54. 55. © USLU B, 1996, TURK J CHEM, V20, P323 WALSH MI, 1983, ANALYST, V108, P626 WALSH MI, 1988, TALANTA, V35, P320 WANG RY, 1999, J CHROMATOGR B, V721, P327 WANG RY, 2001, J SEP SCI, V24, P658 ZHANG Q, 2005, INT J PHARM, V302, P10, DOI 10.1016/j.ijpharm.2005.05.043 For pre-prints please write to: abubakridris@hotmail.com Copyright: King Fahd University of Petroleum & Minerals; http://www.kfupm.edu.sa