1. Introduction to The Genomics Sciences Lab Services and Support

2. The GSL operates as a solution center and provides CHHE and the NCSU community, collaborators, and the scientific community with access to HTS, genotyping and support services In addition to serving as a research resource, the GSL is committed to the education and training of CHHE students, faculty, and staff. GSL General Mission

3. GSL MISSION for CHHE High-throughput sequencing technology at NCSU GSL will support CHHE researchers to conduct Complete transcriptome analysis Transcriptome development Gene expression analysis) ChIPseq Gene mutation analysis SNP analysis DNA methylation miRNA analysis eQTL analysis Genotyping by Sequencing Genome wide association studies

4. AIM 1. Provide expertise in study design and sample preparation. The Genomics Section will provide guidance in all areas of high-throughput sequencing services, including assistance with sample and project preparation, and implementation of protocols for sequencing on various platforms. AIM 2. Provide high-throughput sequencing services. The Genomics Section will provide high-throughput sequencing technology and instrumentation, library preparation, and consultation for all levels of experimental design, development, and assessment. AIM 3. Provide guidance and expertise on Big Data analysis. Provide guidance and expertise on Big Data analysis. The GSL is linked to NC State’s Bioinformatics Research Center (BRC) and large dataset management and analysis will be conducted at the BRC by coordinating with the STC Bioinformatician when needed. GSL AIMS for CHHE

5. Support from start to finish – AIMS 1-3 Experiment design Experiment execution Tissue collection Nucleic acid extraction Nucleic acid QC Library synthesis Sequencing Data QC Data analysis Publication

6. Experiment design Experiment execution Tissue collection Nucleic acid extraction Nucleic acid QC Library synthesis Sequencing Data QC Data analysis Publication Choosing the best platform(s), pooling and lane use Watch and learn, FREE for CHHE - TruSeq, RiboZero, NebNext etc. Low input, Ultra low input Support from start to finish – AIMS 1-3

7. http://research.ncsu.edu/gsl AIM 2

9. + NextSeq500!! Coming soon!

10. HiSeq MiSeq PacBio These are cartoons of reads!! Choosing the best platform – AIM 1

11. HiSeq MiSeq PacBio Choosing the best platform – AIM 1

12. HiSeq MiSeq PacBio Error type Low complexity/diversity Accuracy Starting quantity Choosing the best platform – AIM 1

13. Sanger Sequencing Is your tube Single and alone? Bring it to us! For the LOW price of $4.00 your tube can be SEQUENCED and finally find a home! *full plates too! *free pick-up service *data delivered 4-5pm

14. Other equipment and services

15. http://research.ncsu.edu/gsl