1. 94VG 175 300 Insert size M 94PCRG##.13 450 210 280 80 800 13 1 1 11 1 1 PCR gel template: Fill in the #s and Xs with the appropriate information 650 1000 1650 2000 4000 500 400 300 850 200 100 3000 94AS 94MK 94PV 94KR 94SM 94VK

2. 94RS 320 550 Insert size 94PCRG##.13 570 700 750 680 500 M 1 1 11 1 1 PCR gel template: Fill in the #s and Xs with the appropriate information 650 1000 1650 2000 4000 500 400 300 850 200 100 3000 94PS 94RB 94PP 94SP 94AK 94SN 13

3. 1. Delete the gel picture in this slide. 2. Paste/import cropped gel here and resize to fit markers (note: some markers and lanes may need to be moved to fit the gel image). 3. Label lanes with the proper clone names and add student names (e.G. Change #XX to 20WS and the # to your clone number 1, 2, or 3). 4. Indicate the calculated size of the insert by pcr at the bottom of the gel (e.G. ### To 500 bp). Note: remember to subtract the vector length 200 bp. 5. Save file as a.Jpeg with the proper pcrg name i.E. 20pcrg1.11 6. Upload file to your school’s PCRG album on picasa. 7. Link the image of the file to your clones on the google docs clone report sheet