2. AP Biology Lecture #30 History of the Molecular Basis for Inheritance

3. The Molecular Basis of Inheritance

4. Scientific History The march to understanding that DNA is the genetic material – T.H. Morgan (1908) – Frederick Griffith (1928) – Avery, McCarty & MacLeod (1944) – Erwin Chargaff (1947) – Hershey & Chase (1952) – Watson & Crick (1953) – Meselson & Stahl (1958)

5. The “Transforming Principle” 1928 Frederick Griffith – Streptococcus pneumonia bacteria was working to find cure for pneumonia – harmless live bacteria (“rough”) mixed with heat-killed pathogenic bacteria (“smooth”) causes fatal disease in mice – a substance passed from dead bacteria to live bacteria to change their phenotype “Transforming Principle”

6. The “Transforming Principle” Transformation = change in phenotype something in heat-killed bacteria could still transmit disease-causing properties live pathogenic strain of bacteria live non-pathogenic strain of bacteria mice diemice live heat-killed pathogenic bacteria mix heat-killed pathogenic & non-pathogenic bacteria mice livemice die A.B. C. D.

7. DNA is the “Transforming Principle” Avery, McCarty & MacLeod – purified both DNA & proteins separately from Streptococcus pneumonia bacteria which will transform non-pathogenic bacteria? – injected protein into bacteria no effect – injected DNA into bacteria transformed harmless bacteria into virulent bacteria 1944 What ’ s the conclusion? mice die

8. Avery Experiment

9. Oswald AveryMaclyn McCartyColin MacLeod Avery, McCarty & MacLeod Conclusion – first experimental evidence that DNA was the genetic material 1944 | ??!!

10. Confirmation of DNA Hershey & Chase – classic “blender” experiment – worked with bacteriophage viruses that infect bacteria – grew phage viruses in 2 media, radioactively labeled with either 35 S in their proteins 32 P in their DNA – infected bacteria with labeled phages 1952 | 1969 Hershey Why use S ulfur vs. P hosphorus?

11. Protein coat labeled with 35 S DNA labeled with 32 P bacteriophages infect bacterial cells T2 bacteriophages are labeled with radioactive isotopes S vs. P bacterial cells are agitated to remove viral protein coats 35 S radioactivity found in the medium 32 P radioactivity found in the bacterial cells Which radioactive marker is found inside the cell? Which molecule carries viral genetic info? Hershey & Chase

13. Blender experiment Radioactive phage & bacteria in blender – 35 S phage radioactive proteins stayed in supernatant therefore viral protein did NOT enter bacteria – 32 P phage radioactive DNA stayed in pellet therefore viral DNA did enter bacteria – Confirmed DNA is “transforming factor” Taaa-Daaa!

14. Hershey & Chase Alfred HersheyMartha Chase 1952 | 1969 Hershey

15. Chargaff DNA composition: “Chargaff’s rules” – varies from species to species – all 4 bases not in equal quantity – bases present in characteristic ratio humans: A = 30.9% T = 29.4% G = 19.9% C = 19.8% 1947 That ’ s interesting! What do you notice? Rules A = T C = G

16. Structure of DNA Watson & Crick – Credited with double helix model of DNA other leading scientists working on question: – Rosalind Franklin - crystallography – Maurice Wilkins - crystallography – Linus Pauling – alpha helix in proteins 1953 | 1962 Franklin WilkinsPauling

17. Watson and Crick 1953 article in Nature CrickWatson

18. Rosalind Franklin (1920-1958)

19. Double helix structure of DNA “It has not escaped our notice that the specific pairing we have postulated immediately suggests a possible copying mechanism for the genetic material.” Watson & Crick

20. Directionality of DNA You need to number the carbons! – it matters! OH CH 2 O 4 5 3 2 1 PO 4 N base ribose nucleotide This will be IMPORTANT!!

21. The DNA backbone Putting the DNA backbone together – refer to the 3 and 5 ends of the DNA the last trailing carbon OH O 3 PO 4 base CH 2 O base O P O C O –O–O CH 2 1 2 4 5 1 2 3 3 4 5 5 Sounds trivial, but … this will be IMPORTANT!!

22. Nucleotides in DNA backbone are bonded from phosphate to sugar between 3 & 5 carbons – DNA molecule has “direction” – complementary strand runs in opposite direction 3 5 5 3 Anti-parallel strands

23. Bonding in DNA ….strong or weak bonds? How do the bonds fit the mechanism for copying DNA? 3 5 3 5 covalent phosphodiester bonds hydrogen bonds

24. Base pairing in DNA Purines – adenine (A) – guanine (G) Pyrimidines – thymine (T) – cytosine (C) Pairing – A : T 2 bonds – C : G 3 bonds

25. Large groove Small groove 1 Twist = 10.5 bp 1 2 3 4 5 6 7 8 9 10 36 Å Characteristics of Double Helix 3’ 5’ 3’