1. AP Biology Chapter 9

2. Griffith 1928 Proved transformation of bacteria into a mouse Had two strains of bacteria –An avirulent or nonlethal strain (R) –A virulent of lethal strain (S)

3. Griffith’s Experiment When he put the virulent strain in the mouse, it died When he put the avirulent strain in the mouse, the mouse lived! Then he heated the virulent strain and then put it into the mouse and the mouse lived! When he put the heated virulent strain + the nonvirulent strain into the mouse, the mouse died  Why?

5. Explanation Transformation had occurred The nonvirulent bacteria took up something in the dead heated virulent strain: a “transforming principle” and changed the nonvirulent strain into a virulent strain!!

6. Other Scientists of interest Before 1940, biologists thought that proteins were the information molecules because they were so complex and had a lot of variety Avery, Macleod and McCarty in 1944 proved that the transformation principle in Griffith’s experiment was DNA!

7. Other Scientists of interest Hershey and Chase in 1952 proved that bacteriophages (viruses that attack bacteria) inject DNA into bacterial cells Franklin and Wilkins used x ray diffraction on DNA to determine the distances between molecules Watson and Crick in 1953 came up with the model of DNA

8. Chargaff’s rules He found a simple relationship in DNA called the base pairing rules –Adenine = Thymine –Guanine = Cytosine

9. Watson and Crick Crick: English phage geneticist at the Cavendish labs at Cambridge University, London England Watson: American postdoc in Crick’s lab Both visited Wilkins & Franklin routinely 1951-53 Derived the overall concept of the chemical relationship Considered how Chargaff’s rules represented the structure of DNA Franklin’s X ray data Built little tin models of the nucleotides and put the DNA model together like a TinkerToy set Correctly deduced the structure of DNA (double helix)

11. The Double Helix This is the Watson and Crick model worked out in 1953 and published in a single-page article in Nature of that year. Was convincing structurally: gave evidence for how DNA replicated Most famous biology paper ever written!

12. DNA Structure Called deoxyribonucleic acid Made up of nucleotides which have 3 parts 1.sugar – deoxyribose 2.Phosphate 3.Nitrogen base

13. Deoxyribose Pentose sugar = 5 carbons Carbons on the sugar are numbered 1 through 5 and the first carbon (1’) is linked to one of the four nitrogen bases (ACTG)

14. phosphate Is attached to the 5’ and 3’ carbon, making a phosphodiester linkage Forms the sugar phospate backbone of DNA (or the ladder)

15. Nitrogen base Remember these are connected to the 1’ carbon of the sugar 2 groups –Purines Have two ring structures Adenine and guanine –Pyrimidines Have one ring structure Thymine and cytosine The number of purines = number of pyrimidines

18. DNA molecule Consists of 2 polynucleotide chains arranged in a coiled double helix Helix is like a ladder Two strands run in opposite directions and are said to be antiparallel to each other –The nitrogen bases are bonded by hydrogen bonds –A = T and C=G according to Chargaff’s rules

20. Hydrogen bonds

21. What is the complement of 3’AGCTAC5’?

22. How Does DNA Replicate? Several research groups worked on this. We’ll discuss one 1957: Matthew Meselsohn and Fred Stahl They had 3 hypotheses

23. 1.DNA replication is “semiconservative” –One old strand kept with each of the new molecules; one old paired with one new strand

24. 2. DNA replication is “conservative” –Double strand maintained intact; new strands are together in the new molecule

25. 3. DNA replication is “dispersive” –Strands cut up and the old and new DNA interspersed in both new strands

26. Semiconservative is correct! Each strand acts as a template for the other, and so the mutation will propagate through successive generations.

27. How Does Replication Start? The replication complex binds at the origin of replication, which is identified by a particular base sequence. This is initiated by RNA primer Helicase unwinds the DNA, which is held open with helix-destabilizing proteins. Replication starts in the Y-shaped replication fork.

28. Replication Proceeds on Two Strands Nucleotides are always added to the 3’ end by DNA polymerase, thus moving in the 3’ to 5’ direction but the new strands elongate in opposite directions The leading strand elongates into the fork The lagging strand elongates away from the fork Elongation proceeds smoothly on the leading strand

29. Leading and Lagging Strands As the fork grows, both new strands elongate further Subunit addition to the lagging strand is by 100- 2000 base Okazaki fragments. The lagging strand grows in a discontinuous manner because of the size of the Okazaki fragments That’s why it lags

30. The Lagging Strand Notice that the lagging strand is always growing away from the replication fork The gaps between the Okazaki fragments are joined together by DNA ligase

31. In Action!

32. Review of Enzymes Unwinds the DNA Puts down RNA primer Adds bases to strands Seals Okazaki fragments up Winds the DNA molecule back together

33. DNA Repair DNA polymerase proofreads what bases had been laid down If there is a mistake, it will go back and remove the wrong base and fix it

34. How does DNA fit in the cell? By histones –Positively charged proteins (due to the high number of amino acids) –Are able to associate with DNA which is negatively charged (due to the phosphate groups) Histones and DNA form structure called nucleosome

35. Nucleosome Are 8 histones with DNA wrapped around it Are part of the chromatin Prevent DNA strands from becoming tangled

36. Cellular Ageing and DNA The replication process never entirely completes at the ends of the chromosomes However, DNA is protected at its ends with long strands that do not carry any genetic information, called telomeres as we age, they become shorter They are repaired and lengthened with an enzyme called telomerase Loss of telomerase activity may be an important cause of cellular aging