1. Plenty of Planaria Laboratory Investigation The Science and Ethics of Stem Cell Research

3. What are Planaria? Freshwater flatworms (phylum: Platyhelminthes) Live in freshwater under leaves and stones Avoid light (“negative phototaxis”) Free living – not parasites Carnivores – eat decaying meat, insects and larvae (egg yolks or liver in the lab) 1-20 mm in size Long lived Use sexual or asexual reproduction This picture has been released it into the public domain.

4. Overview of Brown Planaria Dugesia tigrina

5. Planaria are complex organisms Gastrovascular System

6. Nervous System

7. Reproductive System

8. Excretory/Osmoregulatory System

9. Why study Planaria to learn about stem cells? Planaria have an amazing ability to regenerate parts of themselves Planaria regeneration is a great model for demonstrating stem cell function and complexity of tissue regeneration They can grow easily in the lab 1/279th

10. Neoblasts = Planaria Stem Cell (Dev. Biol 220: 142-153, 2000) 20-30% of all the cells in a planaria are Neoblasts. They are present throughout the planaria (except far anterior & pharynx)

11. Neoblasts Neoblasts divide by mitosis and can make more of themselves. They are the only dividing cells in planaria. When a planaria is wounded, neoblasts migrate to the site and begin dividing Neoblasts can become ANY cell the planaria needs—nerve cells, flame cells, reproductive cells… Ann. Rev. Cell. Dev. Biol. 20:725-757, 2004

12. Neoblasts and Stem Cells are Totipotent “Totipotent” Stem Cell Blood Nerve Skin They can become any cell type in the body In Humans: Totipotent cells are limited to early embryonic division In Planaria: Neoblasts are always present

13. turning on the right genes at the right time in the cells that come from the dividing stem cells (neoblast). Regeneration requires…

14. Student Laboratory Investigation Each team will receive 3 planaria: Experimental Planaria #1 Every team will cut this into half, separating the anterior from the posterior halves. Experimental Planaria #2 This will be cut in a manner determined by your team. Control Planaria This will not be cut. Used with permission from UC Museum of Paleontology

15. Plenty of Planaria

16. Care and Feeding of Planaria The water is very important! Fresh water from a pond, stream or lake is best; spring water is adequate Keep cultures in a cool, dark place Feed planaria at least twice a week Change the water after feeding After cutting them, the cultures should not be fed