9 Ligate Multiplex PE adapters SPRI bead purificationLigated samples checked on DNA 1000 LabChip (please refer previous slides)
10 Custom synthesised Blocks used for Hyb Probes designed on eArrayAdapter linked gDNA fragmentsSureSelect Biotinylated RNA BaitsHybridisation 65C for 24h)Streptavidin coated magnetic beadsHybrid capture using MPCUnbound fragmentsCustom synthesised Blocks used for Hyb
11 Post hybridisation PCR involve indexing of libraries Quantification of adapted and enriched libraries on High Sensitivity LabChip
12 Equimolar pooling of libraries 50bp Multiplex PE Illumina sequencingData Analyses
13 The data was aligned with hg18 genome build using bowtie Size of the target region covered by SureSelect Human X-Chromosome baits is3,053,381 bp.Total length of the captured sequence is 3,025,546 bp (99.09%).