1. Supplemental Figure 1 Relative intensities 6008001000 0 Acetone Chloroform Ethanol Hexane Isopropanol Acetic acid Methanol t-BME Toluene Water Xylene No wash 616.28, Heme group Mass-to-charge (m/z) 780800 796.5 790 810 500700 9001100

2. 0 0.5 1.0 1.5 2.0 2.5 EthanolIsopropanolAcetic acidWaterNo wash Relative TIC Day 1 Day 2 Day 4 Day 7 Day 14 Supplemental Figure 2

3. 5000100001500020000 0 1000 2000 3000 4000 5000 Ethanol Isopropanol Acetic acid Water 5000100001500020000 0 1000 2000 3000 4000 5000 Relative intensities Day 2 Day 7 Mass-to-charge (m/z) Supplemental Figure 3

4. Mass-to-charge (m/z) 5000100001500020000 0 1000 2000 3000 Relative intensities Day 2 Day 4 Day 7 Day 14 4000 Day of Analysis Relative Protein TIC 0 2468101214 0.2 0.4 0.6 1.0 0.8 Supplemental Figure 4