1. Warm-Up 2/23 -2/24 Choose one of the three enzymes from DNA replication. Explain what it is doing, including the molecules it is working on (substrate) and the terms active site and activation energy. I am stamping 12.2 Book Notes and the Strawberry Lab

2. DNA Replication Journal Activity Line up according to your confidence with DNA Replication Wait for me to give you a partner Critique each other’s journal writes for: All 3 enzymes and what they do Labeling of 5’ and 3’ ends of all strands An explanation of why original strand one’s copy can be built continuously while original strand two’s must be built in fragments The finished product

3. Transcription and Translation: Making Proteins

4. Making a protein DNA contains the directions for assembling amino acids (they make up proteins). DNA cannot leave the nucleus Protein synthesis occurs outside of the nucleus, on a ribosome. Ribosome How do we get directions to the ribosome?

5. Using RNA RNA is used to send the information to the ribosomes Ribonucleic acid (RNA): Is a single strand only Is made of a nucleotide containing a ribose sugar, a phosphate and a nitrogenous base: Uracil (instead of Thymine), Adenine, Cytosine and Guanine.

6. Transcription Transcription: making an RNA copy of DNA to send to the ribosomes Copy is disposable! Step One: RNA Polymerase (enzyme) unwinds DNA double helix Step Two: RNA Polymerase builds a complimentary strand of messenger RNA (mRNA) using the DNA template

7. Once the mRNA is made, the double helix winds back up!

8. Self Test!!! You are making a strand of mRNA using the following DNA template: TTCGCAAT What would the order of the mRNA strand be?

9. Transcription movie http://www.youtube.com/watch?v=5MfSYnItYvg &feature=relmfu

10. Translation Translation: nucleotides on mRNA are translated into amino acids (and then proteins) This occurs on a ribosome

11. Steps of Translation 1. mRNA moves from nucleus to the ribosomes 2. Ribosomal RNA (rRNA) on the ribosome binds to the mRNA and starts “reading” codons. Codons – 3 letter piece of mRNA that specifies a particular amino acid Ex: AGU or GCG is a codon

12. Ribosome has two parts (large and small) and two binding sites

13. Steps of Translation 3. Transfer RNA (tRNA) links amino acids together and transfers them to the ribosome to make a protein - tRNA contains an anti-codon on one side and an amino acid on the other - an anti-codon is the opposite of the three letter code of mRNA

15. Steps of Translation 4. The ribosome releases the protein and the mRNA - protein to be used by the cell - mRNA to synthesize more protein http://www.youtube.com/watch?v=8dsTvBa UMvw&feature=relmfu

16. In Summary… Transcription (making mRNA) and Translation (making amino acids from mRNA) is how DNA makes protein Sections of DNA are called ‘genes’, and they make certain traits by coding for proteins Those proteins go out and build the traits of living things!

17. mRNA-amino acid chart Knowing a DNA strand, we can predict the amino acids in the protein that is made This chart helps us out: Watch a demo on the board