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DNA replication Understand the basic rules governing DNA replication Introduce proteins that are typically involved in generalised replication Reference:

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Presentation on theme: "DNA replication Understand the basic rules governing DNA replication Introduce proteins that are typically involved in generalised replication Reference:"— Presentation transcript:

1 DNA replication Understand the basic rules governing DNA replication Introduce proteins that are typically involved in generalised replication Reference: Any of the recommended texts Optional Nature (2003) vol 421,pp

2 `It has not escaped our notice that the specific pairing we have postulated immediately suggests a possible copying mechanism for the genetic material Watson & Crick Nature (1953) Original drawing by Francis Crick

3 Four requirements for DNA to be genetic material Must carry information Cracking the genetic code Must replicate DNA replication Must allow for information to change Mutation Must govern the expression of the phenotype Gene function

4 Much of DNAs sequence-specific information is accessible only when the double helix is unwound Proteins read the DNA sequence of nucleotides as the DNA helix unwinds. Proteins can either bind to a DNA sequence, or initiate the copying of it. Some genetic information is accessible even in intact, double- stranded DNA molecules Some proteins recognize the base sequence of DNA without unwinding it (One example is a restriction enzyme). DNA stores information in the sequence of its bases

5 DNA replication occurs with great fidelity Somatic cell DNA stability and reproductive-cell DNA stability are essential. Why? Pan troglodytes 99% sequence identity Identity Genetic diseases Homo sapiens sapiens 99.9% sequence identity

6 DNA Replication Process of duplication of the entire genome prior to cell division Biological significance extreme accuracy of DNA replication is necessary in order to preserve the integrity of the genome in successive generations In eukaryotes, replication only occurs during the S phase of the cell cycle. Replication rate in eukaryotes is slower resulting in a higher fidelity/accuracy of replication in eukaryotes

7 Basic rules of replication A.Semi-conservative B.Starts at the origin C.Synthesis always in the 5-3 direction D.Can be uni or bidirectional E.Semi-discontinuous F.RNA primers required

8 DNA replication 3 possible models

9 Semi-conservative replication: One strand of duplex passed on unchanged to each of the daughter cells. This 'conserved' strand acts as a template for the synthesis of a new, complementary strand by the enzyme DNA polymerase

10 How do we know that DNA replication is semiconservative? Meselson-Stahl experiments

11 B) Starts at origin Initiator proteins identify specific base sequences on DNA called sites of origin Prokaryotes – single origin site E.g E.coli - oriC Eukaryotes – multiple sites of origin (replicator) E.g. yeast - ARS (autonomously replicating sequences) Prokaryotes Eukaryotes

12 In what direction does DNA replication occur? Where does energy for addition of nucleotide come from? What happens if a base mismatch occurs? C) Synthesis is ALWAYS in the 5-3 direction

13 Why does DNA replication only occur in the 5 to 3 direction? Should be PPP here

14 D) Uni or bidirectional Replication forks move in one or opposite directions

15 E) Semi-discontinuous replication Anti parallel strands replicated simultaneously Leading strand synthesis continuously in 5– 3 Lagging strand synthesis in fragments in 5-3

16 Semi-discontinuous replication New strand synthesis always in the 5-3 direction

17 F) RNA primers required

18 Topoisomerases Helicases Primase Single strand binding proteins DNA polymerase Tethering protein DNA ligase - Prevents torsion by DNA breaks - separates 2 strands - RNA primer synthesis - prevent reannealing of single strands - synthesis of new strand - stabilises polymerase - seals nick via phosphodiester linkage Core proteins at the replication fork

19 The mechanism of DNA replication Arthur Kornberg, a Nobel prize winner and other biochemists deduced steps of replication Initiation Proteins bind to DNA and open up double helix Prepare DNA for complementary base pairing Elongation Proteins connect the correct sequences of nucleotides into a continuous new strand of DNA Termination Proteins release the replication complex

20 The mechanism of DNA replication Life: 7 th ed - Chapter 11

21 Core proteins at the replication fork Nature (2003) vol 421,pp Figure in Big Alberts too

22 What kind of enzyme synthesizes the new DNA strand? 1)RNA polymerase 2)DNA Polymerase 3)Primase 4)Helicase 5)Topoisomerase

23 Eukaryotic chromosomes have multiple origins of replication 1.True 2.False

24 In what direction is the newly synthesized DNA produced? 1.5'-3' 2.3'-5' 3.In the direction of the major groove 4.Both 5'-3' and 3'-5' depending on which strand is being replicated

25 Nucleotides are always added to the growing DNA strand at the 3 end, at which the DNA has a free ______ on the 3 carbon of its terminal deoxyribose. 1.Phosphate group 2.Hydroxyl group 3.Nitrogen base 4.Methyl group

26 The E. coli chromosome has 4.7x10 6 bp; a bi-directional replication fork progresses at about 1000 nucleotides/sec. Therefore, the minimum time required to complete replication is 1)12 min. 2)24 min. 3)39 min 4)78 min 5)120 min

27 What is the sequence (1 to 6) in which these proteins function during DNA replication ____ RNA primase ____ DNA ligase ____ DNA polymerase ____ Topoisomerase ____ DNA helicase ____ tethering proteins

28 Why is an RNA primer necessary for DNA replication? A.The RNA primer is necessary for the activity of DNA ligase. B.The RNA primer creates the 5 and 3 ends of the strand. C.DNA polymerase can only add nucleotides to RNA molecules. D.DNA polymerase can only add nucleotides to an existing strand


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