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Aspergillus Classic Diagnostic Dr. Patricio Godoy Martínez Universidad Austral de Chile

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Presentation on theme: "Aspergillus Classic Diagnostic Dr. Patricio Godoy Martínez Universidad Austral de Chile"— Presentation transcript:

1 Aspergillus Classic Diagnostic Dr. Patricio Godoy Martínez Universidad Austral de Chile

2 Aspergillus Ubiquitous, commonly ocurring in soil, water, and decaying vegatation. Reservoirs in hospitals; unfiltered air, ventilation system, carpeting, food, water distribution system, etc. Cause invasive and allergic disease in humans and other animals. Cause plant and food spoilage and produce mycotoxins.

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7 Classification of Aspergillus. ~260 especies, 38 have caused disease Balajee & Marr,2006. Future Microbiology, 1:

8 Geiser Medical Mycology, 47:S21-S26 Sexual stages associated with the genus Aspergillus

9 Aspergillus Direct Indirect Mycology Diagnostics

10 Aspergillus Microscopic examination Colony Detection of Ag and A.N Direct Methods Coloration Histopathology Coloration Histopathology

11 Direct microscopic examination hyphaes hyalines septate. Vitale R

12 Histopathology: H&E Vitale R

13 Histopathology: Grocott Vitale R

14 Identification

15 Macro-microscopic observation of colonies

16 Morphological character

17 COLUMNAR RADIATE Morphological character Josep Guarro

18 Aspergillus fumigatus Colonies black blue-green, consisting of a dense felt of conidiophores. www. pgodoy. com

19 Aspergillus fumigatus Conidial heads columnar. Conidiogenous cells uniseriate. Conidiophore stipes smooth-walled often green in the upper part. Conidia verrucose.

20 Neosartorya Anamorphs closely related to A. fumigatus. Teleomorphs only differentiated by subtle differences in ascospores ornamentation. Thermophilic.

21 Neosartorya N. fischeri N. pseudofischeri N. hiratsukae

22 Neosartorya Osteomyelitis Keratitis Endocarditis Peritonitis Cerebral infection Pulmonary infection Disseminated infection

23 Neosartorya pseudofischeri

24 Neosartorya hiratsukae Josep Guarro

25 N. pseudofischeri N. hiratsukae Josep Guarro

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27 Neosartorya Underdiagnosed (regarded as contaminants in the lab) Difficult to identify at species level. Antifungal susceptibility similar to A. fumigatus ? Little experience in the treatment. Amphotericin B and itraconazole Josep Guarro

28 Aspergillus niger Colonies black, consisting of a dense felt of conidiophores. www. pgodoy. com

29 Aspergillus niger Conidial heads radiate. Vesicles subspherical, conidiogenous cells biseriate. Conidia brown, ornamented.

30 Aspergillus flavus Colonies yellowish-green, consisting of a dense felt of conidiophores.

31 Aspergillus flavus Conidial heads radiate. Conidiogenous cells uni and biseriate. Conidiophore stipes rough-walled hyaline. Conidia echinulate. Sclerotia may be present.

32 Aspergillus parasiticus R. Salim & R. Runco Colonies yellowish-brown, consisting of a dense felt of conidiophores. conidiophores of varying lengths, wall hyaline, thick, with ornamentation equinulada, spherical vesicles, uni or biseriate (24-25 m), completely covering the fiálides gallbladder. Conidia globose or subglobosos warted (2.5-3 m) with thin walls (20, 21).

33 Aspergillus tamarii Conidial heads compact and spherical or loosely radiate. Conidiogenous cells uni and biseriate. Conidiophore stipes rough-walled hyaline. Conidia echinulate.

34 Aspergillus orizae Conidial heads radiate to loosely columnar. Conidiogenous cells uni and biseriate. Conidiophore stipes rough-walled hyaline. Conidia smooth-walled to roughened.

35 Aspergillus terreus Colonies yellowish-brown to cinnamon-brown, consisting of a dense felt of conidiophores.

36 Aspergillus terreus Conidial heads densely columnar. Conidiogenous cells biseriate. Conidiophore stipes smooth-walled hyaline. Conidia smooth walled, striate hyaline.

37 Aspergillus nidulans Colonies growing rapidly, green, cream-buff or honey-yellow, reverse dark purple. Vitale R

38 Aspergillus nidulans Conidial heads short, columnar. Conidiogenous cells biseriate. Conidiophore stipes brownish. Conidia spherical, rugulose, subhyaline, green. Vitale R

39 Aspergillus clavatus Colonies growing rapidly, bluish-green, consisting of a dense felt of conidiophores.

40 Conidial heads radiate, later splitting into several columns. Conidiogenous cells uniseriate. Conidiophore stipes smooth. Vesicle clavate. Conidia smooth-walled hyaline. Aspergillus clavatus

41 INFOCUS CHILE


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