Presentation on theme: "Dr. Patricio Godoy Martínez Universidad Austral de Chile"— Presentation transcript:
1Dr. Patricio Godoy Martínez Universidad Austral de Chile AspergillusClassic DiagnosticDr. Patricio Godoy MartínezUniversidad Austral de Chile
2AspergillusUbiquitous, commonly ocurring in soil, water, and decaying vegatation.Reservoirs in hospitals; unfiltered air, ventilation system, carpeting, food, water distribution system, etc.Cause invasive and allergic disease in humans and other animals.Cause plant and food spoilage and produce mycotoxins.
3This is a scanning electron micrograph of a mold called Aspergillus niger ((As-pur-jillis’ ny’-jur) at 35 times magnification. This is the black stuff you see growing in the grout in a shower. Notice how it looks like an island with funny looking trees? The tree trunks are called hyphae (hy’-fee), and the leafy area is the conidophore (kon-i’-do-for).
4This is the conidophore at 350 times magnification Each separate round “leaf” is a fungal spore. The spore is the reproductive part of the mold, much like a seed to a plant. If this were a video clip instead of a slide, you’d see the hyphae tree trunks waving back and forth on the island, dropping spore coconuts all over the place.
5This is a picture of the spores at 3500 times magnification This is a picture of the spores at 3500 times magnification. Ok -they look more like cheerios strung together than coconuts. The point is, each spore has the capability of starting it’s own island, which is one of the reasons why it’s so hard to remove and control these organisms.
27Neosartorya Underdiagnosed (regarded as contaminants in the lab) Difficult to identify at species level.Antifungal susceptibility similar to A. fumigatus ?Little experience in the treatment. Amphotericin B and itraconazoleJosep Guarro
28Aspergillus nigerColonies black, consisting of a dense felt of conidiophores.www. pgodoy. com
30Aspergillus flavusColonies yellowish-green, consisting of a dense felt of conidiophores.
31Aspergillus flavusConidial heads radiate. Conidiogenous cells uni and biseriate. Conidiophore stipes rough-walled hyaline. Conidia echinulate. Sclerotia may be present.
32Aspergillus parasiticus R. Salim & R. RuncoAspergillus parasiticusColonies yellowish-brown, consisting of a dense felt of conidiophores.conidiophores of varying lengths, wall hyaline, thick, with ornamentation equinulada, spherical vesicles, uni or biseriate (24-25 m), completely covering the fiálides gallbladder. Conidia globose or subglobosos warted (2.5-3 m) with thin walls (20, 21).
33Aspergillus tamariiConidial heads compact and spherical or loosely radiate. Conidiogenous cells uni and biseriate. Conidiophore stipes rough-walled hyaline. Conidia echinulate.
34Aspergillus orizaeConidial heads radiate to loosely columnar. Conidiogenous cells uni and biseriate. Conidiophore stipes rough-walled hyaline. Conidia smooth-walled to roughened.
35Aspergillus terreusColonies yellowish-brown to cinnamon-brown, consisting of a dense felt of conidiophores.