Presentation on theme: "Aspergillus Classic Diagnostic Dr. Patricio Godoy Martínez Universidad Austral de Chile"— Presentation transcript:
Aspergillus Classic Diagnostic Dr. Patricio Godoy Martínez Universidad Austral de Chile
Aspergillus Ubiquitous, commonly ocurring in soil, water, and decaying vegatation. Reservoirs in hospitals; unfiltered air, ventilation system, carpeting, food, water distribution system, etc. Cause invasive and allergic disease in humans and other animals. Cause plant and food spoilage and produce mycotoxins.
Classification of Aspergillus. ~260 especies, 38 have caused disease Balajee & Marr,2006. Future Microbiology, 1:
Geiser Medical Mycology, 47:S21-S26 Sexual stages associated with the genus Aspergillus
Aspergillus Direct Indirect Mycology Diagnostics
Aspergillus Microscopic examination Colony Detection of Ag and A.N Direct Methods Coloration Histopathology Coloration Histopathology
Direct microscopic examination hyphaes hyalines septate. Vitale R
Histopathology: H&E Vitale R
Histopathology: Grocott Vitale R
Macro-microscopic observation of colonies
COLUMNAR RADIATE Morphological character Josep Guarro
Aspergillus fumigatus Colonies black blue-green, consisting of a dense felt of conidiophores. www. pgodoy. com
Aspergillus fumigatus Conidial heads columnar. Conidiogenous cells uniseriate. Conidiophore stipes smooth-walled often green in the upper part. Conidia verrucose.
Neosartorya Anamorphs closely related to A. fumigatus. Teleomorphs only differentiated by subtle differences in ascospores ornamentation. Thermophilic.
Neosartorya N. fischeri N. pseudofischeri N. hiratsukae
Neosartorya Underdiagnosed (regarded as contaminants in the lab) Difficult to identify at species level. Antifungal susceptibility similar to A. fumigatus ? Little experience in the treatment. Amphotericin B and itraconazole Josep Guarro
Aspergillus niger Colonies black, consisting of a dense felt of conidiophores. www. pgodoy. com
Aspergillus flavus Colonies yellowish-green, consisting of a dense felt of conidiophores.
Aspergillus flavus Conidial heads radiate. Conidiogenous cells uni and biseriate. Conidiophore stipes rough-walled hyaline. Conidia echinulate. Sclerotia may be present.
Aspergillus parasiticus R. Salim & R. Runco Colonies yellowish-brown, consisting of a dense felt of conidiophores. conidiophores of varying lengths, wall hyaline, thick, with ornamentation equinulada, spherical vesicles, uni or biseriate (24-25 m), completely covering the fiálides gallbladder. Conidia globose or subglobosos warted (2.5-3 m) with thin walls (20, 21).
Aspergillus tamarii Conidial heads compact and spherical or loosely radiate. Conidiogenous cells uni and biseriate. Conidiophore stipes rough-walled hyaline. Conidia echinulate.
Aspergillus orizae Conidial heads radiate to loosely columnar. Conidiogenous cells uni and biseriate. Conidiophore stipes rough-walled hyaline. Conidia smooth-walled to roughened.
Aspergillus terreus Colonies yellowish-brown to cinnamon-brown, consisting of a dense felt of conidiophores.