1. Dr. Parvin Pasalar Tehran University of Medical Sciences دانشگاه علوم پزشكي وخدمات بهداشتي درماني تهران

2. Objectives: To know and explain  What are needed for Replication  Template properties  Building Blocks  Enzymes  Protein Factors   Different Stages of Replication   Initiation  Elongation  Termination  Difference between Replication in eu and pro  Toxins and antibiotics that inhibit replication

3. Replication( overall view) During cell division Whole DNA must replicate During cell division Whole DNA must replicate The DNA double helix unwinds The DNA double helix unwinds The exposed bases bind to free floating nucleotides in the nucleoplasm The exposed bases bind to free floating nucleotides in the nucleoplasm DNA polymerase binds the complimentary nucleotides DNA polymerase binds the complimentary nucleotides

5. 1- Permission (licensing) 2- Template ( Double Stranded DNA) 3- Building blocks ( dNTPs & NTPs) 4- Enzymes 5- Energy ( Energy of dNTPs & ATP) 6- Protein Factors What are needed for Replication

6. The Permission

7. The Permission: Methylated DNA in the origin, can be distinguished from the replicated DNA

8. The template It is a double stranded DNA It should have a specific sequence (origin) for replication to be started It is replicated in semiconservative manner It is replicated bidirectionaly

9. Replication / The origin Replication starts from Origin Replication starts from Origin Origin has specific AT rich sequences Origin has specific AT rich sequences Because of the shape of the Because of the shape of the origin, replicating bacteria DNA is called teta form 4 of 9 mer 3 of 13 mer OriTeta form A minimal Origin sequence is consisted of : 245bp

10. Enzymes of Replication 1- DNAPs ( DNA synthesis, gap filling) 2- RNAP =primase ( priming DNA synthesis) 3- Helicases ( opening the helix turns) 4- Topoisomerase (removing the superheix turns) 5- Ligase ( sealing the nicks between Okazaki fragments)

11. DNA polymerase I III II 5‘ 3‘ 5‘ 3‘ polymerase Exonuclease ( Proof reading) exonuclease ＋＋＋ ＋＋＋ ＋－－ TNO( nt/min) 600309000 Mass (kDa) 103 90 900 Numbers/ cell 400 10-20??? Bioactivity 10.0515 Gene pol C ＊ pol A pol B DNA Polymerases in Bacteria

12. DNAPs can not initiate DNA synthesis (no de novo synthesis) and there must be small pre-existing primersDNAPs can not initiate DNA synthesis (no de novo synthesis) and there must be small pre-existing primers The enzyme responsible for primer synthesis is called Primase It synthesize the primer from 5 ’ to 3 ’It synthesize the primer from 5 ’ to 3 ’ It has not exonuclease activityIt has not exonuclease activity It is part of primosomeIt is part of primosome 2- Enzymes/b: Primase( Dna G)

13. 2- Enzymes/c: Topoisomerases Enzymes which relieve stress on the DNA by allowing free rotation around a single or double stranded DNA There are two classes: 1 & 2 Class 2 in bacteria is called gyrase. Gyrase reduces two turn of DNA each time ( convert +ve superhelix into – ve)

14. 2- Enzymes/d: Helicases There are different types of helicases : Dna B Other Helicases Enzyme which catalyze the unwinding and separation ( bearking H-bonds) of the parental double helix by using ATP

15. Ligases seal nicks in DNA The energy is provided by ATP or NAD Phage--ATP E.coli--NAD * T 4 DNA ligases can join two blunt DS DNA 2- Enzymes/e: Ligase Eukaryote--ATP

16. D Dna A direct primosome to the origin na B( Helicase activity) na C & C’ are co-activator for primase na G( primase) S SSBP binds to each ss DNA, keep the separated strands apart T Tus recognize the ter sequence for termination of replication Protein Factors of Replication

18. Different Stages Recognition of the Ori sequence by Dna A Recognition of the Ori sequence by Dna A Initiation by primosome Initiation by primosome Elongation by replisome Elongation by replisome Termination at ter sequence by tus protein Termination at ter sequence by tus protein

19. Initiation of replication at Ori C

20. 1- Recognition of the origin by Dna A molecules 2- Twisting of DNA around them result in a short unwinded DNA( loop) 3- Progressive unwinding of the DNA by Dna B and eye loop formation 4-Assembly of primosome at replication origin 5- Synthesis of the primer by primase followed by DNAP activity Recognition & Initiation of replication

21. 2- Elongation: 5 ’ to 3 ’ PDE bond formation Replisome, a protein complex, associate with particular DNA structure to unwind the DNA and synthesis daughter strands. Pyrophosphate hydrolysis is necessary for irreversibility of this reaction

22. The two strands are run in opposite direction The DNA synthesis is only from 5 ’ to 3 ’ The two strands are synthesized Simultaneously But how ? One strand is made continuously (leading strand) and The other is made discontinuously (lagging strand, Okazaki fragments) Simultaneous synthesis of two strands of DNA

23. OKAZAKI FRAGMENT Its structure: A short sequence(10 nt) of RNA primer + about 100 nt of DNA How it is made: Primer by primase and DNA by DNAP III How it is joined to the other fragments: First the primer of the previous Okazaki fragment is hydrolyzed and replace by DNAP I, then the two ends of DNA is linked by ligase

24. Priming Extension Removal of primer Gap filling Nick ligation Discontinuous Replication Stages 1- Primer synthesis by primase 2- DNA synthesis by DNAP III 3- Removal of primer by DNAP I 4- DNA synthesis by DNAP I 5- Sealing the nick between two Okazaki fragments by ligase Nick translation: is referred to these last three stages

25. How the two strands are synthesize in same direction?

26. 3- Termination of replication in E.Coli 1- Ter sequences is a consensus sequences with 23 bases 2- Tus protein is a 36 kDa protein that binds to the ter site 3- Contra helicase activity of the Ter- Tus complex 4- DNA replication stop 5- Decatenation by topoisomerase

27. The fidelity of DNA replication Control at two different stages: 1- Presynthetic control: Control at the incoming base 2- Proofreading: Substitution Determined by 3 ’ to 5 ’ exonuclease activities

29. Replication in Eukaryotes The differences between Pro and Eu The Replication rate in eukaryotes could be low at least because: 1- DNA is so long 2- There are many physical barriers BUT How to overcome this problem? Having hundreds of origins

30. There are hundreds of Origin in eukaryotic genome, each is called ARS ARS (Autonomous replicating sequence)

31. There is a rate-limiting component which function only once at the origin--licensing factor Prevent more than one cycle of replication--by removing the component Two purposes Makes the replication initiation dependent on cell division Licensing factor

32. DNA polymerase δ replicase Priming replication repair location α βγε Nu Mito repair function replication Different DNAPs In Eukaryotic 5‘-3’ plolymerization 3‘-5’ exonuclease ＋＋＋＋＋ －－＋＋＋

33. Different Stages Recognition of the ARS sequence by ORC Recognition of the ARS sequence by ORC Initiation by DNAP α Initiation by DNAP α Elongation by replisome Elongation by replisome Termination by telomerase Termination by telomerase

34. Elongation Nucleosome problem

35. End Replication Problem

36. Toxins and antibiotics that inhibit replication : makes cross link between the two strands of DNA, preventing them of being template Mitomycine : makes cross link between the two strands of DNA, preventing them of being template Nalidixic acid : Prevents gyrase activity Nalidixic acid : Prevents gyrase activity