1. DNA

2. DNA or Protein the Genetic material?? Hershey-Chase Experiment http://highered.mcgraw- hill.com/sites/0072437316/student_view0/ chapter14/animations.html# http://highered.mcgraw- hill.com/sites/0072437316/student_view0/ chapter14/animations.html#

3. Structure: Built of nucleotides: Pentose sugar Pentose sugar Phosphate Phosphate Nitrogen base Nitrogen base Purines – adenine, guanine Pyrmidines – thymine, cytosine N base attached to 1’ C of sugar 3’ C of 1 sugar bonds to 5’ phosphate to form phosphodiester bond http://207.207.4.198/pub/flash/24/menu.sw f http://207.207.4.198/pub/flash/24/menu.sw f

5. Chargaff’s Rules # of purines = # pyrimidines A = T C = G From here, conclusion that adenine H- bonds with thymine and cytosine H-bonds with guanine

6. Complementary Strands DNA arranged in double helix (Rosalind Franklin’s work) Antiparallel – run 5’  3’ on 1 strand and 3’  5’ on other 2 strands are complementary; ie 3’—AGTAC—5’ 3’—AGTAC—5’ 5’—TCATG—3’ 5’—TCATG—3’

8. DNA Replication is semiconservative Confirmed by Messelson-Stahl: http://highered.mcgra w- hill.com/sites/007243 7316/student_view0/c hapter14/animations. html# http://highered.mcgra w- hill.com/sites/007243 7316/student_view0/c hapter14/animations. html#

9. Steps in DNA Replication Begins at specific sites called origins of replication DNA helicase unwinds double helix by breaking H-bonds – forms replication forks Single-stranded binding proteins hold strands open

10. DNA polymerases add nucleotides to 3’- end of growing DNA strand Synthesis is always in 5’  3’ direction Synthesis is always in 5’  3’ direction Requires a RNA primer to build off of Requires a RNA primer to build off of DNA primase synthesizes a short complementary RNA strand for DNA polymerase to build on to DNA primase synthesizes a short complementary RNA strand for DNA polymerase to build on to

11. DNA Replication occurs on both strands at the same time Strands run in opposite directions DNA only replicates in 5’  3’ direction Therefore, only 1 strand can replicate toward the replication fork: leading strand Strand replicating away from the fork is called the lagging strand Can only synthesize short pieces at a time Can only synthesize short pieces at a time Okazaki fragments Okazaki fragments

13. Synthesis on leading strand is continuous Synthesis on lagging strand requires multiple primers When RNA primer of previous Okazaki fragment is reached, DNA polymerase breaks it down DNA ligase seals Okazaki fragments together http://207.207.4.198/pub/flash/24/menu.swf http://highered.mcgraw- hill.com/sites/0072437316/student_view0/chapte r14/animations.html# http://highered.mcgraw- hill.com/sites/0072437316/student_view0/chapte r14/animations.html#

14. Proofreading DNA polymerase proofreads new nucleotides against template If mistake made, DNA polymerase repairs mistake If mistake not corrected, mutation has occurred

15. Pro vs. Eukaryotes Prokaryotes have 1 origin of replication DNA synthesis proceeds in both directions around the circular chromosome until 2 replication forks meet DNA synthesis proceeds in both directions around the circular chromosome until 2 replication forks meet Eukaryotic chromosomes have multiple origins of replication Replication bubbles eventually meet and merge Replication bubbles eventually meet and merge Speeds up process Speeds up process

17. Telomeres End of each chromosome is left short, unreplicated strands of DNA These ends are repeating, non-coding sections called telomeres Help to regulate the # of times of cell can divide Help to regulate the # of times of cell can divide Cancer cells possess telomerase, which adds repeating sequences to ends of chromosomes Cancer cells possess telomerase, which adds repeating sequences to ends of chromosomes