Your new job requires some tools Biotech Inc. does research into developing products for Genetic Engineering, DNA fingerprinting and Cloning As interns you will learn how to use some new tools and techniques Please open your tool box and lets see what’s inside it.
Restriction Enzymes –Act like scissors –Cut DNA at specific places
Recognition sites of different restriction enzymes
Manipulating organisms to create products Use restriction enzymes, ligase, Gel Electrophoresis and PCR Inserts foreign genes into bacteria –Makes bacteria produce proteins –Bacteria can be used to store genes
Plasmid Ligation Puts DNA from humans (or other species) into bacteria DNA.
Review of Bacteria Structure Nucleoid Region- is bacteria chromosome PLASMID- small rings of DNA that have only a few genes. Plasmid
Forensic Scientists Use restriction enzymes, Gel Electrophoresis, PCR Can identify any person based on their DNA sequences. Each of us has unique sequences.
What is an RFLP? Sometimes mutations occur which cause recognition sites to be changed. If a person has a mutation, then the site is changed and DNA of different lengths is produced using the same restriction enzyme. Restriction Fragment Length Polymorphism
Ethics of cloning Occurs naturallly (identical twins) Easier screening of embryos for genetic diseases Increase chances for infertile couples to have children High risk of developmental errors leading to miscarriages Cells are as old as the individual they are taken from