What is the purpose of the GFP in bacterial transformation?
To identify successful transformation and create a glowing protein to be expressed as a result of plasmid uptake
How does the DNA move through the gel plates and how are they represented?
The longer DNA fragments remain at the negative end of the plate because they are too large to move further down the plate. The smaller DNA fragments move more freely towards the positive end of the plate because they contain less base pairs.
what are the five conditions needed to maintain Hardy Weinberg equilibrium?
No genetic drift No gene flow Large population No mutation Random mating
what are the two Hardy Weinberg equations and what do the p and the q represent?
P+q=1 shows the allele frequencies p 2 +2pq+q 2 shows the genotypic frequencies P is the dominant Q is the recessive
An investigator observes that when pure breeding, long-wing Drosophila are mated with pure breeding, short wing flies, the F1 offspring have an intermediate wing length. When several intermediate wing length flies are allowed to interbreed the following results are obtained: 230 long wings 510 intermediate length wings 260 short wings Determine the mode of inheritance and calculate the Chi-Square to test your hypothesis.
Autosomal recessive with incomplete dominance Chi-Square = 2.2 2 degrees of freedom at 0.05 probabilty = Accept Pheno# O# E(O-E)(O-E)^2(o- e)^2/e Long230250204001.6 Int510500101000.2 Short260250101000.4