1. A novel method for the in vivo isolation of circulating tumor cells from peripheral blood of cancer patients using a functionalized and structured medical wire Presenter ： Wei-Siang Ciou Adviser ： Dr.Yi-Chun Du Date ： 2014/11/19 IBS_LAB Saucedo-Zeni, N., Mewes, S., Niestroj, R., Gasiorowski, L., Murawa, D., Nowaczyk, P.,... & Lücke, K. (2012). A novel method for the in vivo isolation of circulating tumor cells from peripheral blood of cancer patients using a functionalized and structured medical wire. International journal of oncology,41(4), 1241-1250.

2. Outline  Abstract  Introduction  Materials and methods  Results  Discussion IBS_LAB

3. Abstract (1/2)  Circulating tumor cells  To overcome the limitations of small blood sample volumes of the ex vivo CTC isolation techniques IBS_LAB

4.  Suitability 、 specificity and sensitivity  Tested in 24 breast cancer or non-small cell lung cancer patients and in 29 healthy volunteers  Found to be safe with no noteworthy side effects Abstract (2/2) IBS_LAB

5. Introduction (1/4)  Personalized cancer treatment  Improve health and quality of life of cancer patients  Different tumor cells are distinct on the molecular level IBS_LAB

6. Introduction (2/4)  Circulating tumor cells(CTCs) CTCs detection flow chart http://www.gendiscovery.com.tw/pdf/ 檢測循環腫瘤細胞研究進展.pdf IBS_LAB

7. Introduction (3/4)  Alternative medical device IBS_LAB

8. Introduction (4/4)  Photoacoustic flow cytofluorometry  Schematic drawing of an in vitro flow system for repeated interaction of CTC with the FSMW, and cancer cells captured by the FSMW. IBS_LAB

9. Materials and methods (1/7)  Functionalized structured medical wire  0.5 mm in diameter and 160 mm in length  The first 20 mm are plated with a 2 µm thick gold layer deposited on the device by galvanization  A hydrogel layer composed of a linear IBS_LAB

10. Materials and methods (2/7)  In vitro experiments IBS_LAB

11. Materials and methods (3/7)  Principle of peristaltic pump IBS_LAB

12. Materials and methods (4/7)  Principle of peristaltic pump IBS_LAB

13. Materials and methods (5/7)  Cytotoxicity tests  Acute systemic toxicity IBS_LAB

14. Materials and methods (6/7)  Hemocompatibility test  Assessment of the patient's risk to develop thrombosis  Coagulation and Hemolysis after in vivo exposure to the FSMW IBS_LAB

15. Materials and methods (7/7)  In vivo application of the FSMW IBS_LAB

16. Result (1/5) IBS_LAB

17. Result (2/5)  Cancer cells captured by the FSMW IBS_LAB

18. Result (3/5)  Biocompatibility of the FSMW device A.Teflon wire eluate ： No apparent changes in cell morphology. B.Copper wire eluate ： Severe changes in cell morphology. C.FSMW eluate prepared from a singl FSMW ： No apparent change in cell morphology. D.FSMW eluate prepared from three FSMW: No apparent change in cell morphology. IBS_LAB

19. Result (4/5)  In vivo application of the FSMW in healthy volunteers and in breast cancer and NSCLC patients IBS_LAB

20. Result (5/5) IBS_LAB

21. Discussion (1/3)  The main cause of death from cancer  Most histopathologic studies investigated primary tumor tissues only IBS_LAB

22. Discussion (2/3)  CTC in the peripheral blood of cancer patients exist in extreme rarity IBS_LAB

23. Discussion (3/3)  Prospective clinical cancer trials. IBS_LAB

24. Thanks for Your Attention IBS_LAB