1. Oxidative phosphorylation Biochemistry, 4 th edition, RH Garrett & CM Grisham, Brooks/Cole (Cengage); Boston, MA: 2010 pp 592-629 Instructor: Kirill Popov

2. 1.The mitochondrion 2.Electron transport 3.Oxidative phosphorylation 4.Heat, oxidative stress, etc.

8. Standard Reduction Potentials of Respiratory Chain and Related Electron Carriers Redox reaction (half-reaction)E'° (V) 2H + + 2e − → H 2 -0.414 NAD + + H + + 2e − → NADH-0.320 NADP + + H + + 2e − → NADPH-0.324 NADH dehydrogenase (FMN) + 2H + + 2e − → NADH dehydrogenase (FMNH 2 )-0.30 Ubiquinone + 2H + + 2e − → ubiquinol0.045 Cytochrome b (Fe 3+ ) + e − → cytochrome b (Fe 2+ )0.077 Cytochrome c 1 (Fe 3+ ) + 2e − → cytochrome c 1 (Fe 2+ )0.22 Cytochrome c (Fe 3+ ) + 2e − → cytochrome c (Fe 2+ )0.254 Cytochrome a (Fe 3+ ) + 2e − → cytochrome a (Fe 2+ )0.29 Cytochrome a 3 (Fe 3+ ) + 2e − → cytochrome a 3 (Fe 2+ )0.35 1/2O 2 + 2H + + 2e − → H 2 O0.8166

10. The Protein Components of the Mitochondrial Electron-Transfer Chain Enzyme complex/proteinMass (kDa)Number of subunits*Prosthetic group(s) I NADH dehydrogenase85043 (14)FMN, Fe-S II Succinate dehydrogenase1404FAD, Fe-S III Ubiquinone:cytochrome c oxidoreductase 25011Hemes, Fe-S Cytochrome c # 131Heme IV Cytochrome oxidase16013 (3-4)Hemes, Cu A, Cu B *Numbers of subunits in the bacterial equivalents in parentheses. # Cytochrome c is not part of an enzyme complex; it moves between Complexes III and IV as a freely soluble protein.

26. The P/O ratio is an index of the efficiency of coupling P/O ratio: number of molecules of P i incorporated (=ATP synthesized) per atom of oxygen consumed (or pair of electrons being carried through the chain). Measurements: oxygen consumption during complete phosphorylation of a fixed amount of ADP after addition of either an NAD + -linked substrate or FAD-linked substrate. P/O  2.5 for NADH P/O  1.5 for FADH 2

30. ATP Yield from Complete Oxidation of Glucose ProcessDirect productFinal ATP Glycolysis 2 NADH (cytosolic) 2 ATP 3 or 5* 2 Pyruvate oxidation (two per glucose) 2 NADH (mitochondrial matrix)5 Acetyl-CoA oxidation in citric acid cycle (two per glucose) 6 NADH (mitochondrial matrix) 2 FADH 2 2 GTP 15 3 2 Total yield per glucose30 or 32 *The number depends on which shuttle system transfers reducing equivalents into the mitochondrion.