9The Cell TheoryWhen Schleiden and Schwann proposed the cell theory in 1838, cell biology research was forever changed. The cell theory states that:
10Cell theory All life forms are made from one or more cells. Cells only arise from pre-existing cells.The cell is the smallest form of life.
11The cell theory also provides us with an operational definition of "life".
12Which of the following is not a part of the cell theory? A.All animals are formed by cells.B.Reproduction requires vegetative duplication or the sexual mixing of gametes.C.Cells are the smallest form of life.D.Abnormal cells self destruct by apoptosis.
14Living things are classified in six kingdoms based on structure. Monera (Eubacteria) ProkaryoticArchaea ProkaryoticProtista EukaryoticPlantae EukaryoticFungae EukaryoticAnimalia Eukaryotic
15Within prokaryoteswhich appeared 3.5 billion years ago, are the kingdomsMonera (Eubacteria) and Archaea.
16Within eukaryoteswhich evolved 1.5 billion years ago, are the kingdomsProtista, Plantae, Fungae, Animalia.
17Cells are also defined according the need for energy.
18Autotrophsare "self feeders" that use light or chemical energy to make food. Plants are an example of autotrophs.
19heterotrophsIn contrast, heterotrophs ("other feeders") obtain energy from other autotrophs or heterotrophs. Many bacteria and animals are heterotrophs.
20Multicellular Organisms Multicellular organisms are created from a complex organization of cooperating cells.There must be new mechanisms for cell to cell communication and regulation.
21There also must be unique mechanisms for a single fertilized egg to develop into all the different kinds of tissues of the body.In humans, there are 1014 cells comprising 200 kinds of tissues!
22Cells are classified by fundamental units of structure and by the way they obtain energy. Cells are classified as prokaryotes or eukaryotes
23Prokaryotesprokaryotes include the kingdoms of Monera (simple bacteria) and Archaea.
24Simply stated, prokaryotes are molecules surrounded by a membrane and cell wall. Prokaryotic cells lack characteristic eukaryotic sub cellular membrane enclosed "organelles", but may contain membrane systems inside a cell wall.
25Bacteria & antibiotics The cell wall is the target for antibiotics, as well as for carbohydrates that our immune system uses to detect infection. A major threat to humankind is the antibiotic-resistant strains of bacteria have been selected by overuse of antibiotics.
26If you were bacteriaYou have times as much DNA as a eukaryotic cell.You live in a medium which has a viscosity about equal to asphalt.You have a wonderful "motor" for swimming. Unfortunately, your motor can only run in two directions and at one speed. In forward, you are propelled in one direction at 30 mph. In reverse your motor makes you turn flips or tumble. You can only do one or the other. You cannot stop.
27While you can "learn", you divide every twenty minutes and have to restart your education. You can have sex, with males possessing a sexual apparatus for transferring genetic information to receptive females. However, since you are both going 30 mph it is difficult to find each other.
28Furthermore, if you are male, nature gave you a severe problem Furthermore, if you are male, nature gave you a severe problem. Every time you mate with a female, she turns into a male. In bacteria, "maleness" is an infective venereal disease.Also, at fairly high frequencies, spontaneous mutations cause you to turn into a female.
29Eukaryotes have enslaved some of your "brethren" to use as energy generating mitochondria and chloroplasts.They are also using you as a tool in a massive effort to understand genetics. The method of recombinant DNA is designed to exploit you for their own good.
30The last laugh may be yours The last laugh may be yours. You have spent three and a half billion years practicing chemical warfare. Humans thought that antibiotics would end infectious diseases, but the overuse of drugs has resulted in the selection of drug resistant bacteria. They didn't realize that this was only the first battle, and now the war is ready to begin.Humans think this is their era. A more truthful statement would be that we all live in the age of bacteria.
31EukaryotesBasic structure The basic eukaryotic cell contains the following:plasma membraneglycocalyx (components external to the plasma membrane)cytoplasm (semi fluid)cytoskeleton - microfilaments and microtubules that suspend organelles, give shape, and allow motionpresence of characteristic membrane enclosed subcellular organelles
33Plasma MembraneA lipid/protein/carbohydrate complex, providing a barrier and containing transport and signaling systems.
34NucleusDouble membrane surrounding the chromosomes and the nucleolus. Pores allow specific communication with the cytoplasm. The nucleolus is a site for synthesis of RNA making up the ribosome
35MitochondriaSurrounded by a double membrane with a series of folds called cristae. Functions in energy production through metabolism. Contains its own DNA, and is believed to have originated as a captured bacterium.
36Chloroplasts (plastids) Surrounded by a double membrane, containing stacked thylacoid membranes. Responsible for photosynthesis, the trapping of light energy for the synthesis of sugars. Contains DNA, and like mitochondria is believed to have originated as a captured bacterium.
37Rough endoplasmic reticulum (RER) A network of interconnected membranes forming channels within the cell. Covered with ribosomes (causing the "rough" appearance) which are in the process of synthesizing proteins for secretion or localization in membranes.
38Smooth endoplasmic reticulum (SER) A network of interconnected membranes forming channels within the cell. A site for synthesis and metabolism of lipids. Also contains enzymes for detoxifying chemicals including drugs and pesticides.
39Golgi apparatusA series of stacked membranes. Vesicles (small membrane surrounded bags) carry materials from the RER to the Golgi apparatus. Vesicles move between the stacks while the proteins are "processed" to a mature form. Vesicles then carry newly formed membrane and secreted proteins to their final destinations including secretion or membrane localization.
41Ribosomes Protein and RNA complex responsible for protein synthesis. Vacuoles Membrane surrounded "bags" that contain water and storage materials in plants.
42Cell wallPlants have a rigid cell wall in addition to their cell membranes.
43Peroxisomes or Microbodies Produce and degrade hydrogen peroxide, a toxic compound that can be produced during metabolism
44LysosymesA membrane bound organelle that is responsible for degrading proteins and membranes in the cell, and also helps degrade materials ingested by the cell.
45Cell membrane plasma membrane The cell membrane is the thin nearly invisible structure that surrounds the cytoplasm of the cell.In this section we will talk about its structure and its function.In the image you can see that it is a continuous membrane that completely surrounds the cell.Cell membrane plasma membrane
46There are carrier proteins that regulate transport and diffusion Floating around in the cell membrane are different kinds of proteins. These are generally globular proteins. They are not held in any fixed pattern but instead float around in the phospholipid layer.
47Cell membrane - Function The cell membrane's function, in general, revolves around is membrane proteins. General functions include:Receptor proteins which allow cells to communicate,transport proteins regulate what enters or leaves the cell,and marker proteins which identify the cell
48Cell membrane - Function - Regulation of transport Transport Proteins come in two forms: Carrier proteins are peripheral proteins which do not extend all the way through the membrane. They move specific molecules through the membrane one at a time.
49Channel proteins extend through the bilipid layer Channel proteins extend through the bilipid layer. They form a pore through the membrane that can move molecules in several ways.
50Cell membrane - Function - Carrier Proteins These are carrier proteins. They do not extend through the membrane. They bond and drag molecules through the bilipid layer and release them on the opposite side.
51Symports also use the process of diffusion Symports also use the process of diffusion. In this case a molecule that is moving naturally into the cell through diffusion is used to drag another molecule into the cell. In this example glucose hitches a ride with sodium.
52The cell membrane can also engulf structures that are much too large to fit through the pores in the membrane proteins.This process is known as endocytosis.In this process the membrane itself wraps around the particle and pinches off a vesicle inside the cell.In this animation an ameba engulfs a food particle.
54Exocytosis The opposite of endocytosis is exocytosis. Large molecules that are manufactured in the cell are released through the cell membrane.
55Proteins in the membrane There are carrier proteins that regulate transport and diffusionMarker proteins that identify the cell to other cellsAnd receptor proteins that allow the cell to receive instructions
56NUCLEUS The nucleus is the headquarters of the cell. It regulates all cell activity.It consists of a nuclear envelope, (the outer membrane) and nucleoplasm.In the nucleoplasm you can see chromatin and the nucleolus.
57The nuclear envelope is a double membrane. Is has 4 phospholipid layers. It is also has large pores through which materials pass back and forth.
58Within the nucleus are found chromatin and a structure called the nucleolus. Chromatin is DNA in its active form. It consists of DNA looped around histone proteins.The nucleolus is a knot of chromatin.It is the nucleolus that manufactures ribosomes
59Nucleus - Function The nucleus regulates all cell activity. It does this by controlling the enzymes present.
60The chromatin is composed of DNA. DNA contains the information for the production of proteins.This information is encoded in the 4 DNA bases.Adenine, thymine, cytocine, and guanine.The specific sequence of these bases tells the cell what order to put the amino acids.
61There are three processes that enable the cell to manufacture protein: Replication allows the nucleus to make exact copies of its DNATranscription allows the cell to make RNA working copies of its DNAIn translation the Messenger RNA is used to line up amino acids into a protein molecule
62CytoplasmThe term cytoplasm refers to everything between the cell membrane and the nuclear envelope. It consists of primarily of water. It also contains various organelles as well as salts, dissolved gasses and nutrients.
63Those organelles involved in protein production RibosomesThe endoplastic reticulumThe golgi apparatusLysosomes
64Those organelles involved in energy production MitochondriaChloroplasts
65Specialty organellesthese structures are usually found only in certain kinds of cells. Animal cells generally contain centrioles.Plant cells generally contain storage vacuoles, cell walls, and plastids.Cilia and flagella are found in many different life forms. Be aware that there are many other kinds of living things besides plants and animals.
66CentriolesCentrioles are found only in animal cells. They function in cell division. Notice the 9 sets of 3 arrangement of the protein fibers
67VacuolesVacuoles are large empty appearing areas found in the cytoplasm.They are usually found in plant cells where they store waste.As a plant cell ages they get larger.In mature cells they occupy most of the cytoplasm.
68Cell WallCell walls are the rigid structure found surrounding plant cells. They provide support for the plant
69PlastidsPlastids are large organelles found on plants and some protists but not in animals or fungi. They can easily be seen through a light microscope. Chloroplasts represent one group of plastids called chromoplasts (colored plastids). The other plastids are called leucoplasts (colorless plastids); they usually store food molecules. Included in this group are amyloplasts or starch plastids shown here in potato root cell.
70Cillia and FlagellaThese are hair like extensions off of the cell membrane. Their structures are similar except that cillia tend to be small and numerous and flagella tent to be large and fewer. Their they beat back and forth rhythmically. In unicellular organisms their job is locomotion. In large multicellular organisms their role is to move fluid past the cell. Notice the 9+2 arrangement of the microtubules
71Contractile VacuolesThese organelles are critical in enabling protozoa to combat the effects of osmosis. Protozoa must constantly excrete the water that enters through their membranes.
72The ChloroplastThe Chloroplast is the photosynthesis. It is here that plant cells trap the energy of light and use it to manufacture food proteins for the cell.
73The MitochondrionThe mitochondrion is the site of aerobic respiration. Most of the key processes of aerobic respiration occur across its inner membrane. One theory holds that they evolved from endosymbiotic bacteria.
74The Endoplastic Reticulum Spreading throughout the cytoplasm is the endoplasmic reticulum. It is a folded system of membranes that loop back and forth giving it a very large surface area. This membrane provides a surface area for cell reactions. It is also the Site of lipid production.
75Smooth E. R. has no ribosomes associated with it The rough E. R Smooth E.R. has no ribosomes associated with it The rough E.R. has ribosomes
76RibosomeThe rough E.R. has ribosomes attached to it. This gives it its texture. These ribosomes manufacture proteins for the cell.
77The Golgi ApparatusThe golgi body is responsible for packaging proteins for the cell. Once the proteins are produced by the rough E.R. they pass into the sack like cisternae that are the main part of the golgi body. These proteins are then squeezed off into the little blebs which drift off into the cytoplasm.
83Why are cells these sizes? surface area/volume ratio (SA/V)everything entering cell comes through the plasma membrane (PM)An increased cytoplasmic volume means more stuff must come thru-more glucose -more amino acids more ions more of everything needed in the cell
85Bigger cell meansAn increased volume => relative decline in Surface area to volume ratioPlasma Membrane loses ability to transport enough stuff in/out of cell
86Solutions to the surface Area Problem (1) infoldings (increases SA)(2) outward protrusions (increases SA)note the projections that increase surface area w/o increasing volume muche.g.: microvilli ofintestinal mucosacells
101Giant Plant Cell _ cytoskeleton nucleus and nucleolus mitochondrion chloroplastrough endoplasmic reticulum (focus on function of ribosome)smooth endoplasmic reticulum (focus on function of ER)Golgi complexlysosomesperoxisomesmicrotubules and microfilamentsvacuole_ cytoskeleton
1021. Students research the structure and function of their chosen organelle. 2. Students produce a blueprint describing how they will construct their model and its size.3. Students prepare a class presentation.
103manufacture the subunits that combine to form ribosomes Organelle NucleusFunctionstores the cell's hereditary material, or DNA2. coordinates the cell's activities, which include growth, intermediary metabolism, protein synthesis, and reproduction (cell division).StructureNucleolusmanufacture the subunits that combine to form ribosomes
104OrganellePlasma MembraneFunctionacts as a boundary, holding the cell together and keeping other substances from entering.Structure
105structure Microfilaments are solid rods made of globular proteins OrganelleMicrofilamentFunctionThese filaments are primarily structural in function and are an important component of the cytoskeleton.structure Microfilaments are solid rods made of globular proteinsMicrotubules are found throughout the cytoplasm of all eukaryotic cells (prokaryotes don't have them) and carry out functions, ranging from transport to structural support.straight, hollow cylinders
106Organelle function Structure Cell Wall protects intracellular contents, provides rigidity, is a porous medium for the circulation and distribution of water, minerals, and other nutrients, and houses specialized molecules that regulate growth and protect the plant from disease.
107OrganellefunctionStructureMitochondrion break down carbohydrate and sugar molecules to provide energyoblong shaped organelles
108OrganelleChloroplastFunctioncontains the pigment chlorophyll. It absorbs light energy needed for photosynthesis to occur.Structure
109Organelle Endoplasmic Reticulum Functionmanufactures, processes, and transports chemical compounds for use inside and outside of the cell.It is connected to the double-layered nuclear envelope, providing a pipeline between the nucleus and the cytoplasm.structurenetwork of sacs
110serve as the protein production machinery for the cell structure Organelle RibosomeFunctionserve as the protein production machinery for the cellstructurecomposed of approximately 60 percent RNA and 40 percent protein.Vacuole50-80% of volumeplant cells have a large, single vacuole that stores things, helps in plant growth, and plays an important structural role for the plant.
111Organelle function Structure Peroxisomes spherical and bound by a single membrane. There are several types of microbodies but peroxisomes are the most common.CILLIA/ Flagellalocomotion of organisms. In multicellular organisms, cilia function to move fluid or materials past an immobile cell as well as moving a cell or group of cells.
112Creating the Organelles The Giant Plant Cell is shaped like a giant cube that is 3 meters (300 cm) on each edge. You have been provided with a chart of actual cell organelle sizes.You must, using ratios, calculate the size of your giant organelle so that it is proportionately correct for the huge cell.You also need to research the function of the organelle in order to build it so that it can carry out its role in the giant cell.
113First, you will prepare an architectural design plan that will be evaluated by your teacher. Then you will build your organelle.You will prepare a 3-5 minute report to present to the class.Your organelle will be judged according to the accuracy of its size, the correctness of its details, your explanation of its function
114Equation for determining size of organelle: Size of giant organelle =Actual size of organelleSize of giant cell (300 cm) =Actual size of average plant cell (30mm)
115Basic Requirements Construction of Organelle Oral Presentation All constructed organelles must be 3-dimensional.All organelles must show internal and external details.All organelles must be the correct size for the colossal plant cell.Oral PresentationReports should be about 3-5 minutes long.Each member of the team should do part of the presentation.The presentation should include details about the organelle's structure and function.The presentation should include a discussion about how many of the organelles will be manufactured for the colossal plant cell.The presentation should also include an explanation of the actual and giant size of the organelle.
116CHART OF ACTUAL ORGANELLE SIZES Cell or OrganelleSize in x (10-3 mm Average Plant Cell 30 x Nucleus xNucleolus 2.5 xPlasma Membrane 0.009 x thickMitochondrion 0.2-1 x wide x 3-10 x longChloroplast (other plastids are similar sizes)2 x 5 xRibosome 0.025 xEndoplasmic Reticulum (in most plant cells)0.5 x thick (2 membranes of mm with 0.03 mm compartment between them) Golgi Complex1 x 1 x (membranes have thickness of ER)Vacuole (central) sometimes serves as lysosome50-80% of volume of cellMicrotubules 0.02 x diameterMicrofilaments x diameterLysosomes (in some plant cells) 0.2-2 xPeroxisomes 3 xCell Wall 1-2 x thick
117QuestionsHow is a typical plant cell the same as a prokaryotic cell? How is a typical plant cell different from a prokaryotic cell?How are plant and animal cells similar? How are plant and animal cells different?Discuss the interaction of the mitochondria and the chloroplasts. What substances do each produce that the other uses?Given the size of the organelles, which structures would you expect to see with a typical light microscope (magnifies 400x)? Explain your reasoning.How would a plant cell found in the petal of a flower be different from/similar to a "typical plant cell" such as the Giant Plant Cell?If the giant plant cell was a leaf cell, describe what you would need to add to the cell to help it function.Describe how proteins are synthesized in a plant cell.How is the cellular structure of terrestrial plants different from that of aquatic plants?If you were looking at a root cell, a leaf cell, and a stem cell what differences would you find?How is carbon cycled in a plant? In a plant cell?What happens to the molecules that are formed by the chloroplasts?What happens to the molecules that are formed by the mitochondria?