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Histology Laboratories James D. Jamieson, MD/PhD

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1 Histology Laboratories James D. Jamieson, MD/PhD
Molecules to Systems 2003 Compiled by James D. Jamieson, MD/PhD Thomas L. Lentz, MD No part of this image collection may be distributed outside of the Yale University Intranet.

2 Acknowledgements Sources of Micrographs, Diagrams and Figures
Alberts, B. et al. Molecular Biology of the Cell. 4th Edition, Garland Science, New York, 2002. Gartner, L. P. and Hiatt, J. L. Color Atlas of Histology, Williams & Wilkins, Baltimore, 1994. Kerr, J. B. Atlas of Functional Histology. Mosby, London, 1999. Kessel, R. G. and Kardon, R. H. Tissues and Organs: a text-atlas of scanning electron microscopy. W. H. Freeman, San Francisco, 1979. Lentz, T. L. Cell Fine Structure. W. B. Saunders, Philadelphia, 1971. Lodish, H. et al. Molecular Cell Biology. W. H. Freeman, New York, Mizoguti, H. Color Slide Atlas of Histology. Nihon Shashin Shinbunsha, Tokyo. Young, B. and Heath, J. W. Wheater’s Functional Histology. Churchill Livingstone, Edinburgh, 2000. Micrographs taken by George Palade, Marilyn Farquhar, James D. Jamieson, Nicolai Simionescu, Maya Simionescu, David Castle, Thomas L. Lentz. Web Resources Cushing Library Educational Software/Cell Biology/Several Histology Resources

3 Endocrine Systems Laboratory

4 Anterior Pituitary

5

6 Regulation of Synthesis
of Pituitary Hormones

7 Optic chiasma Hypothlamus Posterior pituitary Stalk Anterior pituitary Pituitary: Sagittal Section Sella turcica

8 Acidophils (Mammotroph, PRL
Somatotroph, GH) Chromophobes Basophils (Corticotrophs,ACTH Thyrotroph, TSH Gonadotroph, FSH, LH) Anterior Pituitary; H&E.

9 Fenestrated capillary
Anterior Pituitary TEM S: somatotroph G: gonadotroph

10 Posterior Pituitary

11

12 Herring bodies Blood vessels Glial cells Posterior Pituitary

13 ADH/Oxytocin Release in Posterior Pituitary

14 Adrenal Cortex

15 Adrenal C: Cortex M: Medulla

16 Blood Supply of Adrenal

17 Capsule ZG ZF Adrenal Cortex: Zona Glomerulosa; aldosterone

18 ZF Adrenal Cortex: Zona Fasciculata; cortisol

19 ZR Adrenal Cortex: Zona Reticularis; weak androgens

20 Adrenal Cortex (ZR) and Medulla (M)

21 Adrenal Cortical Cell

22 Adrenal Medulla

23 Blood vessel Chromaffin cells Adrenal Medulla; epinephrine and norepinephrine

24 Sympathetic nerve fibers
Chromaffin cells with epinephrine granules Adrenal Medulla

25 Thyroid/Parathyroid

26 Follicles Thyroid Gland

27 Follicular epithelial cells
Colloid (thyroglobulin) Thyroid Follicles

28 Follicular epithelial cells
Parafollicular cells/C-cells Thyroid Follicles; C cells: calcitonin, lowers blood Ca++

29 Colloid C cell Thyroid Follicle TEM

30 Parathyroid

31 Parathyroid Thyroid Parathyroid; PTH elevates Blood Ca++

32 Chief (PTH) cells Adipose cells Oxyphil cells Parathyroid

33 Oxyphil cells Chief (PTH) cells Parathyroid

34 Endocrine Pancreas

35 Exocrine pancreas Islets of Langerhans Endocrine Pancreas

36 Exocrine acini Blood vessels Vein Endocrine Pancreas

37 Normal Islet. Immunoperoxidase against glucagon (L) and insulin (R)

38 Islet Immunostained for Insulin

39 Same Islet Immunostained for Glucagon

40 Glucose Homeostasis: Liver and Endocrine Pancreas

41 GI Endocrine System

42 Enteroendocrine Cells: stained for serotonin

43 Gut Endocrine Cells: XS crypt
A: endocrine cells facing lamina propria

44 Lumen of gastric gland Lamina propria Gastric Endocrine Cells: G = gastrin cell

45 Mucosal Neuroendocrine Cells Facing Lumen and Lamina Propria

46 Mucosal Neuroendocrine Cells Facing Lamina Propria Only
Gut Endocrine Cells Facing Lumen and Lamina Propria

47 A Bit of Pathology

48 Thyroid: Graves disease
Thyroid: Graves disease. What is the functional state of the epithelial cells and the colloid? This path. Is due to autoantibodies that mimic TSH. Discuss in terms of the cell biology of the thyroid.

49 Nodular goiter. Thyroid from a person living in Switzerland
Nodular goiter. Thyroid from a person living in Switzerland. What do you see that is different from normal?

50 Hashimoto’s thyroiditis
Hashimoto’s thyroiditis. Note abnormal lymphoid follicle and infiltrating lymphocytes. What might be the cause of this condition?

51 Insulinoma (malignant): Islet cell tumor stained with insulin antibody
Insulinoma (malignant): Islet cell tumor stained with insulin antibody. What symptoms might the tumor produce?

52 Islet adenoma (benign - encapsulated) compared to normal islet (R).

53 Normal parathyroid (L); functional adenoma (R)
Normal parathyroid (L); functional adenoma (R). What might you see on bone density scans?

54 Normal anterior pituitary (L), Adenoma (R)
Normal anterior pituitary (L), Adenoma (R). This tumor is often functional and secretes prolactin. What might symptoms be in a male?

55 Anterior Pituitary: Immunoperoxidase for prolactin; used in diagnosis of functional pituitary adenomas

56 Capsule Adrenal cortical adenoma. Cells resemble those of the zona fasciculata - lots of fat droplets. How might this condition affect this patient’s muscle mass. What hormone would you ask for in a lab test?


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