1. 5th IgCLL Educational Workshop Program ____________________________________________________________ Immunoglobulin Sequence Analysis in Chronic Lymphocytic Leukemia ____________________________________________________________ Wednesday 25 September 2013Program ____________________________________________________________ 19:00Welcome dinner for invited speakers (Hotel International) Thursday 26 September 2013 ____________________________________________________________ 8:50Sarka Pospisilova (CEITEC MU; University Hospital Brno; CZ) Welcome Keynote Lecture 9:00Nicholas Chiorazzi (The Feinstein Institute for Medical Research, New York, US) The turbulent life history of CLL Session I - Modern prognostication in CLL ChairSarka Pospisilova (CEITEC MU; University Hospital Brno; CZ) 9:45Richard Rosenquist ( Uppsala University, Uppsala, SWE) The genomic landscape of CLL 10:10Kostas Stamatopoulos (G. Papanicolaou Hospital, Thessaloniki, GR ) Immunogenetics and prognostication 10:35Paolo Ghia ( Universita Vita-Salute San Raffaele, Milano, IT) Biological prognostic markers: a critical reappraisal 11:00Coffee break 26. 9. – 27. 9. 2013, Hotel International, Husova 16, Brno, Czech Republic

2. Session II - IG sequence analysis in CLL: technical aspects ChairRichard Rosenquist (Uppsala University, Uppsala, SWE) 11:30Frederic Davi (Hðpital Pitié-Salpêtrière, Paris, FR) Mechanisms of immunoglobulin diversity 11:50Anton Langerak (University Medical Center, Rotterdam, NL) From the patient to the sequence: Primers, PCR, Detection of clonality, Sequencing 12:20Marie-Paule Lefranc (Université Montpellier, Montpellier, FR) Online tools for Interpretation of IG sequences and navigation through the IMGT web- resources 12:50Lunch Session III - Interpretation of IG sequences ChairAnastasia Hadzidimitriou (Centre for Research & Technology Hellas, Thessaloniki, GR ) 14:00Veronique Giudicelli (Université Montpellier, Montpellier, FR) How to use the IMGT/V-QUEST tool 14:30Basic exercises I 15:15Solutions Karla Plevova (CEITEC Masaryk University; University Hospital Brno; CZ) 15:45Coffee break 16:15Basic exercises II 17:00Solutions Vasilis Bikos (CEITEC Masaryk University, CZ) 18:00Visit to Mendel Museum, social event 5th IgCLL Educational Workshop Program

3. Friday 27 September 2013 ____________________________________________________________ Session IV - Interpretation of IG sequences: advanced exercises ChairChrysoula Belessi ( General Hospital of Nikea, Athens, GR) 8:30Advanced exercises I 9:15Solutions Lesley Ann Sutton (Uppsala University, Uppsala, SWE) 9:45Advanced exercises II 10:30Solutions Andreas Agathangelidis (Università Vita-Salute San Raffaele, Milano, IT) 11:00Coffee break Session V - IG sequence analysis for everyone ChairPaolo Ghia (Universita Vita-Salute San Raffaele, Milano, IT) 11:30Richard Rosenquist (Uppsala University, Uppsala, SWE) How to report IG sequence data in clinical routine 11:50Kathleen Potter ( University of Southampton, Southampton, UK) Methodological harmonization, guidance Session VI - Where do we go now? ChairKostas Stamatopoulos (G. Papanicolaou Hospital, Thessaloniki, GR) 12:10Fiona Murray (Uppsala University, Uppsala, SWE) Recombinant antibodies in autoimmunity and malignancy: a key to improved understanding of immune processes 12:30Anton Langerak (University Medical Center, Rotterdam, NL) Immunogenetics in the NGS era: challenges and limitations 12:50Sarka Pospisilova (CEITEC MU; University Hospital Brno; CZ) Concluding remarks 13:00Lunch 5th IgCLL Educational Workshop Program