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Toothpaste Brand vs. Bacteria in your mouth By: Nick Merriman.

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Presentation on theme: "Toothpaste Brand vs. Bacteria in your mouth By: Nick Merriman."— Presentation transcript:

1 Toothpaste Brand vs. Bacteria in your mouth By: Nick Merriman

2 Question: How does the brand of toothpaste you use (Prevident 5000 Plus, Pepsodent, Toms of Maine All Natural Organic) affect the number of bacteria on your teeth when brushing for 2 minutes in the morning with the same toothbrush.

3 Hypothesis: If you change the brand of toothpaste you use then the Prevident 5000 Plus will remove more bacteria than Pepsodent or Toms because it is prescription strength toothpaste. If you change the brand of toothpaste you use then the Prevident 5000 Plus will remove more bacteria than Pepsodent or Toms because it is prescription strength toothpaste.

4 Toothpaste Brands

5 Variables Manipulated: brand of toothpaste Manipulated: brand of toothpaste Controlled: amount of time brushing (2 minutes), type of toothbrush, amount of toothpaste used (1 squeeze), time of day (morning) Controlled: amount of time brushing (2 minutes), type of toothbrush, amount of toothpaste used (1 squeeze), time of day (morning) Responding: number of bacteria colonies on your teeth Responding: number of bacteria colonies on your teeth

6 Materials Prevident 5000 Plus: Dry Mouth Prevident 5000 Plus: Dry Mouth Pepsodent Pepsodent Toms of Maine Natural Organic Toms of Maine Natural Organic 6 sheep blood agar culture plates 6 sheep blood agar culture plates 6 sterile swabs 6 sterile swabs 6 sterile inoculating loops 6 sterile inoculating loops toothbrush toothbrush timer timer

7 Materials

8 Procedure Step 1-On the first day when you wake up note the time and brush your teeth for 2 minutes with Prevident 5000 Plus Step 1-On the first day when you wake up note the time and brush your teeth for 2 minutes with Prevident 5000 Plus Step 2-Use the sterile swab to go over your top teeth and your bottom teeth Step 2-Use the sterile swab to go over your top teeth and your bottom teeth Step 3-Using the swab, make a circle on the blood agar about the size of a quarter Step 3-Using the swab, make a circle on the blood agar about the size of a quarter Step 4-Using the sterile loop spread a streak through the circle area you swabbed, and continue spreading the material out in 3 different directions on the plate. This will let you see and be able to count the separate colonies of bacteria. Step 4-Using the sterile loop spread a streak through the circle area you swabbed, and continue spreading the material out in 3 different directions on the plate. This will let you see and be able to count the separate colonies of bacteria.

9 2 minutes is a long time…..

10 Swabbing for the culture

11 Inoculating the agar

12 Procedure continued…. Step 5: Place the blood agar culture in an incubator for 32 hours at 37 degrees Celsius (body temperature) Step 5: Place the blood agar culture in an incubator for 32 hours at 37 degrees Celsius (body temperature) Step 6: Count the number of visible Step 6: Count the number of visible Bacterial colonies growing on the plate and record. Bacterial colonies growing on the plate and record. Step 7: On the second repeat steps 1-6 for trial #2 Step 7: On the second repeat steps 1-6 for trial #2 Step 8: On the third and 4 th day repeat steps 1-7 with Pepsodent Step 8: On the third and 4 th day repeat steps 1-7 with Pepsodent Step 9: On the fifth and sixth day repeat steps 1-7 with Toms of Maine Step 9: On the fifth and sixth day repeat steps 1-7 with Toms of Maine

13 32 hours later…….lots of bacteria

14 Experimental Data Number of Bacterial Colonies on Culture (cfu = colony forming units) Trial #1 Trial #2 Average Prevident 5000 1337 cfu 1363 cfu 1352 cfu Pepsodent 629 cfu 629 cfu 520 cfu 524.5 cfu Toms of Maine 1354 1283 cfu 1318.5 cfu

15 Conclusion My hypothesis was incorrect. The Pepsodent cleaned your teeth the best, not the Prevident 5000 Plus. The low was 520 cfu for the Pepsodent. The high was 1363 cfu for the Prevident 5000 Plus. The difference between the two points was 843 cfu. Therefore I have learned that Pepsodent cleans your teeth the best of these three brands. To take this experiment to the next step, you could explore the different types of bacteria. My hypothesis was incorrect. The Pepsodent cleaned your teeth the best, not the Prevident 5000 Plus. The low was 520 cfu for the Pepsodent. The high was 1363 cfu for the Prevident 5000 Plus. The difference between the two points was 843 cfu. Therefore I have learned that Pepsodent cleans your teeth the best of these three brands. To take this experiment to the next step, you could explore the different types of bacteria.


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