Compounds TNT (2,4,6-Trinitrotoluene) H 2 C=CH-CN Acrylonitrile Carbaryl
Partitioning Separation is based on the analytes relative solubility between two liquid phases Stationary PhaseMobile Phase SolventBonded Phase
HPLC - Modes Normal Phase. - Polar stationary phase and non-polar solvent. Reverse Phase. - Non-polar stationary phase and a polar solvent.
Common Reverse Phase Solvents MethanolCH 3 OH AcetonitrileCH 3 CN Tetrahydrofuran Water H2OH2O
Columns Analytical - Performs the separation. Solid Support - Backbone for bonded phases. –Usually 10µ, 5µ or 3µ silica or polymeric particles. Bonded Phases - Functional groups firmly linked (chemically bound) to the solid support. –Extremely stable –Reproducible Guard - Protects the analytical column: –Particles –Interferences –Prolongs the life of the analytical column