1. TaqMan Gene Expression Arrays for Accurate Quantification of Toxicity Targets

2. Agenda Test Procedure Specifications Validation Results

3. Test Procedure RNA isolation cDNA generation (may be skipped with one-step RT-PCR) Card loading and sealing Thermal cycling Data Analysis

4. Dynamic Range in the Cytokine Card

6. Average plus 6 Stdev is Fold Discrimination Calculate Average of 40 RQ values Normalize RQ values to positive fold Calculate RelQuant Values (20 for each sample--40 total ) Calculate dCt CalibratorCalculate dCT sample 1Calculate dCt sample 2 3 cytokine cards with 20 plasmid targets Fold-Discrimination in the Cytokine Card

8. DNAx: Cytokine Gene Expression and PBMC Activation

9. Source Precision Medicine: Cytokine Gene Expression and PBMC Activation

10. Phase 1 Molecular Toxicology: Cytokine Gene Expression in Liver Cell Cultures

11. Phase 1 Molecular Toxicology: MMC Incubations of Liver Cells in the Human Toxicology Card

12. Phase 1 Molecular Toxicology: MMS Incubations of Liver Cells in the Human Toxicology Card

13. Phase 1 Molecular Toxicology: Carnustine Incubations of Liver Cells in the Human Toxicology Card

14. Phase 1 Molecular Toxicology: Cytokine Gene Expression in RNA Isolated from Blood Cells

15. Phase 1 Molecular Toxicology: Toxicology Gene Expression in RNA Isolated from Blood Cells

16. Phase 1 Molecular Toxicology: Cytokine Gene Expression in RNA Isolated from Lymphocyte Cultures

17. Phase 1 Molecular Toxicology: Toxicology Gene Expression in RNA Isolated from Lymphocyte Cultures