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National Seed Laboratory USDA Forest Service USDA Forest Service National Center for Seed Technology National Center for Seed Technology National Mission.

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Presentation on theme: "National Seed Laboratory USDA Forest Service USDA Forest Service National Center for Seed Technology National Center for Seed Technology National Mission."— Presentation transcript:

1 National Seed Laboratory USDA Forest Service USDA Forest Service National Center for Seed Technology National Center for Seed Technology National Mission National Mission All native plantsAll native plants Protocol development: germ, clean, store seedsProtocol development: germ, clean, store seeds Tech transfer: training, handbooks, individualTech transfer: training, handbooks, individual Seed testing on a fee-for-service basisSeed testing on a fee-for-service basis Germplasm conservation and research seed distributionGermplasm conservation and research seed distribution

2 NSL Germplasm Conservation For FS and FS cooperators For FS and FS cooperators Cooperating w/ ARS Cooperating w/ ARS Fort Collins NCGRP security backupFort Collins NCGRP security backup Capacity to enter accessions into GRIN (FSNS numbers are Forest Service)Capacity to enter accessions into GRIN (FSNS numbers are Forest Service) Wrote FS ash collection plan cooperatively with the Ames PI stationWrote FS ash collection plan cooperatively with the Ames PI station Sharing ash seed and resourcesSharing ash seed and resources

3 NSL Facts Two operational locations Two operational locations Dry Branch GADry Branch GA Hardwood Tree Improvement and Regeneration Center at Purdue W. Lafayette, IN is an additional locationHardwood Tree Improvement and Regeneration Center at Purdue W. Lafayette, IN is an additional location Second base of operations for midwest Second base of operations for midwest Gives NSL better cooperation with FS and university genetics Gives NSL better cooperation with FS and university genetics As a coop service oriented facility, NSL has many contacts in forestry and conservation agencies and organizations. As a coop service oriented facility, NSL has many contacts in forestry and conservation agencies and organizations.

4 Ash Germplasm Collections 2006 2006 start, learned how to do itstart, learned how to do it fair to poor seed year: 20 lots collectedfair to poor seed year: 20 lots collected Began x-raying seed for Rose Lake PMCBegan x-raying seed for Rose Lake PMC Drafted seed collection planDrafted seed collection plan 2007 2007 almost no seed producedalmost no seed produced Some areas had seed: 60 lots collectedSome areas had seed: 60 lots collected 2008 2008 phenomenal seed year: 420+ seed lotsphenomenal seed year: 420+ seed lots Techniques are pretty refined nowTechniques are pretty refined now Cooperative efforts gain tractionCooperative efforts gain traction

5 FS Fraxinus Collection Plan Focused: EAB infestations (EAB viewer) Focused: EAB infestations (EAB viewer) Defined seed collection areas (2 to several counties) Defined seed collection areas (2 to several counties) Derived from EAB viewer, Omernik III regions, species range mapsDerived from EAB viewer, Omernik III regions, species range maps Mostly a way to assign and track collectionsMostly a way to assign and track collections Collections were to more or less be spread across collection areaCollections were to more or less be spread across collection area Worked with Ames PI station to write Worked with Ames PI station to write

6 FS Fraxinus Collection Plan Wild trees, some provision for urban collections Wild trees, some provision for urban collections No selection for phenotype No selection for phenotype Healthy tree but could be EAB attacked Healthy tree but could be EAB attacked Quantity to collect Quantity to collect Min: about 1 quart of seed (1 to 2 inches of seed on bottom of grocery sack)Min: about 1 quart of seed (1 to 2 inches of seed on bottom of grocery sack) Max: Full grocery sackMax: Full grocery sack

7 FS Fraxinus Collection Plan Evaluate seed for weevils – at least 50% good Evaluate seed for weevils – at least 50% good Voucher specimens Voucher specimens Leaf if available (put in data sheet/envelope)Leaf if available (put in data sheet/envelope) Twig sample (placed in the bag w/ the seeds)Twig sample (placed in the bag w/ the seeds) Seeds cluster if possible (put in bag)Seeds cluster if possible (put in bag) PhotosPhotos Close up of bark Close up of bark Whole tree Whole tree

8 Slide the leaf into the envelope diagonally making sure that all the leaflets are flat and not folded over on themselves. This will allow the leaf to dry flat and to be more easily identifiable.

9 Two photos are taken of each tree. One is of the trunk and one is of the full tree. Take the photo of the trunk first. Before photographing the trunk, pin the data sheet to the tree so that the lot number is clearly visible, but off to the bottom in order to show as much of the trunk as possible. Frame both photos vertically as shown here. Taking the photos in this order will ensure the photos can be matched to the correct seed lots. Seed lot number

10 FS Fraxinus Collection Plan Make seeds available for research and restoration Make seeds available for research and restoration Seek out researchers to collaborate with Seek out researchers to collaborate with NSL will distribute NSL will distribute Collecting enough seed to make sure supplies will be sufficient (quart, full bags)Collecting enough seed to make sure supplies will be sufficient (quart, full bags) Seed increase possible, but a long term projectSeed increase possible, but a long term project Objective is the restoration of ash to landscape Objective is the restoration of ash to landscape

11 2006 Cooperative Efforts Attempted to recruit seed collectors from the general public Attempted to recruit seed collectors from the general public Put together kits with all needed supplies and made up a training ppPut together kits with all needed supplies and made up a training pp Did outreach through extension service at Purdue – master gardenersDid outreach through extension service at Purdue – master gardeners Distributed about 12 kits but received no seeds --- needed a more direct approachDistributed about 12 kits but received no seeds --- needed a more direct approach

12 2008 Cooperative Collections Focused on special groups to collect seeds Focused on special groups to collect seeds Received $37M from Forest Health Director Received $37M from Forest Health Director Ash identified as one of 4 top priority species/species groups to be targeted for conservation because of invasive pathogen or pestAsh identified as one of 4 top priority species/species groups to be targeted for conservation because of invasive pathogen or pest Allowed us to buy supplies for our collectorsAllowed us to buy supplies for our collectors Most $$ taken to fight wild fires before we could get it granted to cooperators for travelMost $$ taken to fight wild fires before we could get it granted to cooperators for travel Money was restored so we may grant it this year but money is also needed to clean the 2008 seeds.Money was restored so we may grant it this year but money is also needed to clean the 2008 seeds.

13 2008 Cooperative Collections 20 cooperating entities 20 cooperating entities 2 Arboreta: Holden, Stranahan2 Arboreta: Holden, Stranahan 3 Metro parks in Dayton & Cleveland OH3 Metro parks in Dayton & Cleveland OH 4 Universities: Kent State, Ohio State, Purdue, U MN,4 Universities: Kent State, Ohio State, Purdue, U MN, 3 DNR: PA, IN, OH (urban programs)3 DNR: PA, IN, OH (urban programs) 1 TNC: Blue River Project, IN1 TNC: Blue River Project, IN 2 Hoosier and Chippewa National Forest2 Hoosier and Chippewa National Forest 1 Bloomington IN Parks and Recreation1 Bloomington IN Parks and Recreation 2 Timber companies, 1 private consultants2 Timber companies, 1 private consultants 2 FS Northern Research Station (HTIRC at Purdue, Delaware OH)2 FS Northern Research Station (HTIRC at Purdue, Delaware OH)

14 2008 Cooperative Collections Conducted 6 workshops (4 in OH, 1 IN, 1 MN) with the cooperators Conducted 6 workshops (4 in OH, 1 IN, 1 MN) with the cooperators This focused effort gave a much better result than trying to collect from the general public. This focused effort gave a much better result than trying to collect from the general public. Demonstrated that there are well qualified willing cooperators who with a little more financial support could do much more. Demonstrated that there are well qualified willing cooperators who with a little more financial support could do much more.

15 Other FS Ash Conservation Efforts National Forest System has its own plan to preserve germplasm from NF in the North. National Forest System has its own plan to preserve germplasm from NF in the North. 100 trees per forest per species100 trees per forest per species Submit to the NPGS through the NSL planSubmit to the NPGS through the NSL plan Kim Steiner at Penn State has been given a grant to assess the condition and meaure the 30 year old white and green ash provenance tests. Kim Steiner at Penn State has been given a grant to assess the condition and meaure the 30 year old white and green ash provenance tests. March 2010 HTIRC at Purdue will host a conference on the ash resource March 2010 HTIRC at Purdue will host a conference on the ash resource Document and highlight the importance of the ash resourceDocument and highlight the importance of the ash resource Hopefully develop support for breeding and restoration workHopefully develop support for breeding and restoration work The last two items above are funded by grants from FH The last two items above are funded by grants from FH

16 Needs - technical Assess genetic variation to guide future collections - when do we have enough sources collected? Assess genetic variation to guide future collections - when do we have enough sources collected? Provenance work (funded) Provenance work (funded) Molecular work to describe gene flow, outcrossing patterns, level of variability at the gene level (needs more funding) Molecular work to describe gene flow, outcrossing patterns, level of variability at the gene level (needs more funding) Nursery, breeding, transgenic, and seed orchard research (needed for restoration work) Nursery, breeding, transgenic, and seed orchard research (needed for restoration work) ID sources of EAB resistance ID sources of EAB resistance

17 Needs – Policy/Financial/Adminstrative Need agency leadership support to go beyond the grass roots efforts Need agency leadership support to go beyond the grass roots efforts Need to match up agency resources Need to match up agency resources NSL contributes NSL contributes Seed testing facilities, expertise Seed testing facilities, expertise Conducting workshops Conducting workshops Development of techniques Development of techniques Network of cooperators Network of cooperators Seed storage and distribution facilities Seed storage and distribution facilities How do the various plans fit together? How do the various plans fit together? How do we as a group engage cooperators? How do we as a group engage cooperators? What specific objectives does each agency have? What specific objectives does each agency have? Can we form a unified budget for this project? Can we form a unified budget for this project?


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