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F.D.OVCHARENKO INSITUTE OF BIOCOLLOIDAL CHEMISTRY NATIONAL ACADEMY OF SCIENCES OF UKRAINE Kyiv GOLD-BEARING PROBIOTIC DRUG OKARIN-AU Valeriya VEMBER Telephone:

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Presentation on theme: "F.D.OVCHARENKO INSITUTE OF BIOCOLLOIDAL CHEMISTRY NATIONAL ACADEMY OF SCIENCES OF UKRAINE Kyiv GOLD-BEARING PROBIOTIC DRUG OKARIN-AU Valeriya VEMBER Telephone:"— Presentation transcript:

1 F.D.OVCHARENKO INSITUTE OF BIOCOLLOIDAL CHEMISTRY NATIONAL ACADEMY OF SCIENCES OF UKRAINE Kyiv GOLD-BEARING PROBIOTIC DRUG OKARIN-AU Valeriya VEMBER Telephone: Workshop From Science to Business October, 2006 Kyiv

2 Proprietary information statement The technology material presented in this talk is available for licensing and joint product development. None of the slides contain any confidential or proprietary information. 1

3 The shape of gold-bearing probiotic preparation "Okarin-Au" 2

4 3 increase of stressful factors quantity and strength deterioration of ecological situation uncontrolled usage of antibiotics and other medicines using great amount of junk food Gastrointestinal tract disorders

5 4 B i o t h e r a p y Probiotic foodPrebioticProbiotic substances, which selectively stimulate the growth and biological activity of microorganisms in intestines, which positively influence on the microbiocenosis structure live microorganisms; metabolites produced by some microorganisms to stimulate the growth of other ones, etc. foodstuff bearing by probiotics

6 Main problems as to probiotic drugs development and application : limited antagonistic activity of the strains-producers to the numerous pathogenic agents; insufficient growth speed of biomass producing strains; high cost of probiotic preparation as a result of using the expensive growth substances for strains- producers growing; non-observance of the microecological adequacy principle while treatment of different dysbacteriosis types have been provided 5

7 6 Bringing the appropriate metals into the organism gives good therapeutic results in case of illnesses, which are characterised by considerable changes in metal-microelement balance Probiotic + Prebiotic = Synbiotic M E T A L S important regulatory environmental compounds and important regulatory environmental compounds and potential ingredient for Synbiotics

8 ADVISABILITY of PROBIOTIC DRUG ENRICHMENT by COLLOIDAL Au and Ag Investigation of metals influence on some nonspecific physiological and biochemical parameters, which have been of great importance for all the metabolic processes that take place in bacterial cell : Growth processes intensity Cells wall permeability ATP-ase activity Energy function of bacterial plasma membrane Level of lipid peroxidation Antioxidant enzymes activity 7

9 Changes of biological activity of bacterial association Okarin after incubation with GOLD (10 -6 mkg/ml) and SILVER (10 -7 mkg/ml) MetalsForm Investigated indexes Growth intensity ATP-ase activity H + - extrusion Perme- ability of the cell wall Level of lipid peroxi- dation Activity of antioxidant enzymes CatalaseSOD Au Colloidal 0 Ion Ag Colloidal 0 00 Ion Kind of changes : – positive ; – negative ; «» – investigation is not conducted.8

10 Resistance of Okarin and Okarin-Au to the antibiotics 9

11 Antagonistic activity of bacterial association Okarin and gold-bearing Okarin-Au Microorganisms (test object ) Size of lysis zone after 24 hours, mm Quantity of test-culture ( pieces ) (Х ± m) Control after 12 hours after 24 hours after 72 hours Okarin Okarin- Au Okarin Okarin- Au Okarin Okarin- Au Okarin Okarin- Au Okarin Okarin- Au Shigella flexneri 1а ± ± ± 7 12 ± 2 Sh. flexneri 1в ± ± 8 58 ± 7 14 ± 5 Sh. flexneri 2а ± ± ± ± Sh. flexneri VI ± ± ± ± Sh. sonnei ± ± ± ± Salmonella enteritidis ± ± ± ± S. thyphimurium ± ± ± ± Staphylococcus aureus ± ± ± ± Pseudomonas aeruginosa ± ± ± ± Ps. vulgaris Not find 122 ± ± 2 85 ± 7 34 ± 9 * behaviors, which show the improvement of initial preparation Okarin by colloidal gold. 10

12 Wide range of antagonistic activity to pathogenic microorganisms causing dysbacteriosis and especially dangerous infection diseases including anthrax, rabbit-fever, salmonellosis, brucellosis etc. (according to results of clinical trials); Selective bactericidal activity to pathogenic microflora and simultaneous stimulation of normoflora. This feature is intensified owing to the presence of colloidal gold particles ; Low cost of the treatment course in a comparison with other probiotic drugs which are available on the market today; Ability to increase the lysis of atypical pathological cells treatment application opportunity for oncological patients due to high resistance of probiotic's microbial cultures to antibiotics and chematherapeutic agents; Non-specific immune-resistance stimulation ability of the whole organism; Suitable producing shape and application simplicity. Innovative Aspect and Main Advantages 11

13 Clinical trials results of bacteriological preparation "Okarin-Au" 12 Pathogenous species: Staphylococcus aureus, Enterobacter aerogenes, Klebsiella pneumoniae, Candida albicans Normal flora Normal flora of GIT: Bifidobacterium, Lactobacillus, Escherichia coli Dysbiosis State NORMAL NORMAL State Okarin-Au Standard PGT + Okarin-Au Therapy Standard PG Therapy

14 Competitive Matrix of Probiotic Probiotics Name Probiotics NameProbioticsCharacteristic Okarin-Au (IBCC-UkrInkom, Ukraine) Colibac- terinum (Biofarma, Ukraine) Hylak Forte (Ratiopharm, Germany) Linex (LekPharma, Slovakia) Bifiform (Ferrosan, Denmark) Preparation Composition E. coli (3 strains) + Enterococcus faecalis (dried alive cultures, enriched by colloidal Gold) – 1:1:1:3. Capsules. E. coli (strain M-17) (dried alive cultures) – 1*10 10 CFU/dose. Ampoules. Metabolites of E. coli, Streptococcus faecalis, Lactobacillus acidophilus and L. helveticus + growth factors (aqueous solution) – 2:1:1:4. Bottles. Lactobacillus acidophilus, Bifidobacterium infantis, Enterococcus faecium (dried alive cultures) – 1,2*10 7 CFU/dose (each of strains). Capsules. Bifidobacterium longum and Enterococcus faecium (dried alive cultures) – 1*10 7 CFU/dose (each of strains). Capsules. Antagonistic activity (AA) Shigella flexneri, Sh. sonnei, Salmonella enteritidis, S. thyphimurium, Staphylococ- cus aureus, Pseudomonas aeruginosa, Ps. vulgaris, Enterobacter aerogenes, Klebsiela pneumonia, Candida albicans Mixed infection of GIT and Salmonellosis. Prohibited to use in the case of Escherichiosis !!! Mixed infection of GIT and Salmonellosis Wide range of AA (accordance to preparation Instruction) Wide range of AA (accordance to preparation Instruction Dosage regimen (per 24 hours) 2-3 capsules6-12 doses drops6 capsules2-4 capsules Cost of treatment for 10 days $ 10$ 7$ 11$ 15$ 12,5 Complications and peculiarities of application 1) Require preliminary preparation; 2) Prohibited to use at the same time with Antibiotics 1) Require preliminary measuring; 2) Prohibited to use at the same time with Milk and Dairy Produce 13

15 We seek for potential partners interested in new type of combined (and effective!) probiotic preparation and partners, who can help us in putting them on the market We are ready for collaboration with the purpose of creation of different synbiotic drugs which include wide range of colloidal metals and have different therapeutic effects O p p o r t u n i t i e s 14

16 Contact Information : Prof. Zoya ULBERG Telephone: +380 – 44 – F.D.OVCHARENKO INSITUTE OF BIOCOLLOIDAL CHEMISTRY NATIONAL ACADEMY OF SCIENCES OF UKRAINE Kyiv 15


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