1. DEPARTMENT OF CELL BIOLOGY Head: Dr. Jacek Bigda, Associate Professor Research Staff: Dr. Małgorzata Doszczak Dr. Patrycja Koszałka Dr. Zuzanna Dobrzańska Ph.D. Students: Marcin Okrój Arkadiusz Pierzchalski

2. MAIN TOPIC Mechanism of action of tumor necrosis factor (TNF) in vitro & in vivo TEACHING cell biology, histology, immunology

3. DEPARTMENT OF CELL BIOLOGY research interests:  mechanism of IL-1-induced cytotoxicity;  intracellular signaling of tumor necrosis factor;  mechanism of toxicity of the anti-angiogenic fumagillin derivative, TNP-470;  mechanism of anti-tumor action of poly-RGD peptides;  mechanism of accelerated growth of hamster melanoma following LPS application.

4. U 937 – human histiocytic lymphoma TNF-R55 F N TF N TF N T TNF-R75 F N T m F N T m F N T

5. Cycloheximide (CHX) U937 cell variants

6. U 937 M U 937 G U 937 ATCC

7. IL-1 cytotoxicity

9. control 4 h 2 h 0,5 h 1 h 0 100 200 300 400 500 TNF [pg/ml] control CHX IL-1 IL-1/CHX IL-1 cytotoxicity

10. Isotype control Untreated IL-1/CHX

12. DEPARTMENT OF CELL BIOLOGY financial support:  Analysis of mechanism regulating secretion of TNF following application of interleukin 1 and cycloheximide; State Committee for Scientific Research, Poland (2002-2005);  Mechanism of synergistic anti-tumor action of tumor necrosis factor (TNF) and angiogenesis inhibitor TNP-470; State Committee for Scientific Research, Poland (2001-2004);  Analysis of TNF sensitivity of variants of the U937 cell line; Intramural grant, Medical University of Gdańsk (2002-2003);  Modulation of tumor growth by means of cytokines; Core fund, Intercollegiate Faculty of Biotechnology UG-MUG;

13. DEPARTMENT OF CELL BIOLOGY selected previous grants:  Significance of tumor necrosis factor and its receptors for growth and dissemination of Bomirski melanoma; State Committee for Scientific Research, Poland (1994-1997);  Immunomodulatory and anti-tumor activity of cloned and purified rat tumor necrosis factor; State Committee for Scientific Research, Poland (1997-2000);  Influence of receptors of tumor necrosis factor for apoptosis of cancer cells; State Committee for Scientific Research, Poland (1997-2000);  Mechanism of cytotoxic action of interleukin 1; State Committee for Scientific Research, Poland (1999-2002);  TNF gene polymorphism and humoral reactivity against p53 protein in patients suffering from non-small cell lung cancer; State Committee for Scientific Research, Poland(1999-2002).

14. Tri-City Central Animal Laboratory, Medical University of Gdańsk

15. Tri-CityCentral Animal Laboratory, Medical University of Gdańsk Tri-City Central Animal Laboratory, Medical University of Gdańsk