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Epidemiology and pathogenicity of African bat lyssaviruses Wanda Markotter University of Pretoria South Africa.

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Presentation on theme: "Epidemiology and pathogenicity of African bat lyssaviruses Wanda Markotter University of Pretoria South Africa."— Presentation transcript:

1 Epidemiology and pathogenicity of African bat lyssaviruses Wanda Markotter University of Pretoria South Africa

2

3 DUVV (GT 4) isolates from South Africa

4 DUVV (GT 4) isolates from South Africa Paweska et al., Fatal human infection with rabies-related Duvenhage virus, South Africa. Emerg Infect Dis.2006;12:

5 LBV isolates

6 Several new LBV isolates in from South Africa 4 isolates from fruit bats

7 Several new LBV isolates in from South Africa 4 isolates from fruit bats Markotter W, Randles J, Rupprecht CE, Sabeta CT, Wandeler AI, Taylor PJ, Nel LH. Recent Lagos bat virus isolations from bats (suborder Megachiroptera) in South Africa. Emerg Infect Dis. 2006;12:

8 1 isolate from a mongoose Several new LBV isolates in from South Africa First isolation of LBV from terrestrial wildlife

9 1 isolate from a mongoose Several new LBV isolates in from South Africa First isolation of LBV from terrestrial wildlife Markotter W, Kuzmin I, Rupprecht CE, Randles J, Sabeta CT, Wandeler AI, Nel LH. Isolation of Lagos bat virus from water mongoose. Emerg Infect Dis. 2006;12:

10 1 isolate from a dog Several new LBV isolates in from South Africa

11 11 isolates from South Africa LBV Total: 18 isolates

12 N gene analysis

13 LBV

14 % 99.3% Intrinsic variation

15 Between B and C % Between A and B,C %

16 Between B and C % Between A and B,C % <80% nt identity ? New genotype

17 A value of < 1 indicates an overlap.

18 Pathogenicity i.m. and i.c. i.c.

19 Intracerebral (i.c.) and intramuscular (i.m.) inoculation 1 x 10 2 LD 50 1 x 10 3 LD 50 1 x 10 6 LD 50

20 Conclusions Both DUVV and LBV is endemic in South Africa but not frequently reported due to lack of surveillance Genetic diversity of LBV has been underestimated Molecular phylogeny divided previously reported LBV isolates into three lineages independent of the gene used. Two isolates previously classified as LBV fulfill the criteria to be a new lyssavirus genotype Pathogenic profiles of phylogroup II isolates differ and some isolates indicate the same pathogenicity as a gt 1 representatives Pathogenic characteristics of lyssavirus genotypes or phylogroups should not be decided based on one representative isolate

21 Acknowledgements Prof. Louis H. Nel (UP, South Africa) Dr. Charles E. Rupprecht (CDC, USA) Dr. Ivan Kuzmin (CDC, USA) Dr. Claude T. Sabeta (OVI, South Africa) Dr. Anthony R. Fooks (VLA, UK) Dr. Florence Cliquet (AFFSA, France) Dr. Janusz T Paweska (Special Pathogens, NICD, South Africa) Dr. Robert Swanepoel (Special Pathogens, NICD, South Africa) Miss. Charmaine Wilsenach (UP, South Africa)


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