Presentation on theme: "Stray dogs and rabies in Bangkok, Thailand"— Presentation transcript:
1 Stray dogs and rabies in Bangkok, Thailand Songsri KasempimolpornQueen Saovabha Memorial Institute, Bangkok, Thailand
2 Estimated dog population and vaccine coverage in Bangkok, Thailand Data were compiled by The Bangkok Metropolitan Administration and the Bureau of National StatisticsDog populationHouse dogs , , ,504No. vaccinated , , ,478(67%) (86%) (91%)Stray dogs , , ?No.vaccinated ?? ?? ??
4 Human deaths from rabies, 1997-2006 Year Bangkok*/Thailand/58/57/68/50/37/30/21/19/20/25* Bitten by dogs located in Bangkok
5 Sources of rabies transmitted to man, 2002-2005 Dog81%Not recorded9%Mongoose 5%Rabbit 5%2003Dog93%Not recorded7%2002Not recorded 5%Dog95%2004Dog70%Not recorded20%Monkey 5%Cat 5%2005
6 Rabies virus N gene was digested with enzyme DdeI or HinfI Study of molecular epidemiology of rabies virus that is prevalent among dogs in BangkokABRabies virus N gene was digested with enzyme DdeI or HinfI
7 Canine rabies virus M I II Hf Type I: DdI-HfI Type II: DdII-HfII Type III: DdIII-HfIType IV: DdIV-HfIType V: DdIV-HfIII
13 Determination of rabies antibody in dog serum Enzyme-linked immunosorbent assay (ELISA)- A minimum value of 0.5 IU/ml of neutralizing antibodies should be attained to demonstrate seroconversion in humans, and we used the same for the dogs.- An estimated true prevalence of antibodies among stray dogs was calculated using the Rogan-Gladen-estimator and the confidence interval (CI) was set at 95% (Grenier and Gardner, Prev Vet Med 2000;45:43-59)
15 Detection of rabies virus RNA in saliva specimen by RT-PCR 524 bpDNA Ladder 100 bp+ ve Control- ve ControlDog salivaThe prevalence of rabies virus infection among stray dogs investigated was 0.03% (1 of 3,314)
16 Central Bangkok Bangkok’s outskirts Seroprevalence of rabies antibodies among stray dogs, based on an ELISA cut-off level of 0.5 EU/mlCentral Bangkok Bangkok’s outskirtsNo. tested No. positive Adjusted seroprevalence No. teested No. positive Adjusted seroprevalence% % CI % %CI1, ,The overall seroprevalence of rabies antibody among stray dogs in Bangkok was 62% (95%CI 54-70%)
17 Map of Bangkok depicting the 50 districts Map of Bangkok depicting the 50 districts. Shaded surface areas represent the districts in which the antibody seroprevalence among stray dogs reached 70%
18 Conclusions Rabies infection is still prevalent in Bangkok Low vaccination coverage and ineffective management of stray dogs are the most likely reasons for the dog rabies elimination program’s lack of success
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