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Songsri Kasempimolporn Queen Saovabha Memorial Institute, Bangkok, Thailand Stray dogs and rabies in Bangkok, Thailand.

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Presentation on theme: "Songsri Kasempimolporn Queen Saovabha Memorial Institute, Bangkok, Thailand Stray dogs and rabies in Bangkok, Thailand."— Presentation transcript:

1 Songsri Kasempimolporn Queen Saovabha Memorial Institute, Bangkok, Thailand Stray dogs and rabies in Bangkok, Thailand

2 Estimated dog population and vaccine coverage in Bangkok, Thailand Data were compiled by The Bangkok Metropolitan Administration and the Bureau of National Statistics Dog population House dogs 358, , ,504 No. vaccinated 240, , ,478 (67%) (86%) (91%) (67%) (86%) (91%) Stray dogs 40, ,584 ? No.vaccinated ?? ?? ??

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4 Human deaths from rabies, Year Bangkok*/Thailand Year Bangkok*/Thailand / / / / / / / / / / / / / / / / / / / /25 * Bitten by dogs located in Bangkok

5 Sources of rabies transmitted to man, Not recorded 5% Dog 95% 2004 Dog 93% Not recorded 7%2002 Dog 81% Not recorded 9% Mongoose 5% Rabbit 5% 2003 Dog 70% Not recorded 20% Monkey 5% Cat 5% 2005

6 Study of molecular epidemiology of rabies virus that is prevalent among dogs in Bangkok AB Rabies virus N gene was digested with enzyme DdeI or HinfI

7 Canine rabies virus Type I: DdI-HfI Type II: DdII-HfII Type III: DdIII-HfI Type IV: DdIV-HfI Type V: DdIV-HfIII MIII III (D4,D110) (D38) (D76) Hf

8 Dog Cat

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10 Saliva sample collection was done with a total of 3,314 dogs seen for surgical sterilization during 12/2003-6/2004

11 Detection of rabies virus in dog saliva Screening test: The latex agglutination test (Kasempimolporn et al. J Clin Microbiol 2000;38: ) (Kasempimolporn et al. J Clin Microbiol 2000;38: ) Confirmatory test: RT-PCR with nested primers

12 Blood sample collection

13 Determination of rabies antibody in dog serum Enzyme-linked immunosorbent assay (ELISA) - A minimum value of 0.5 IU/ml of neutralizing antibodies should be attained to demonstrate seroconversion in humans, and we used the same for the dogs. - An estimated true prevalence of antibodies among stray dogs was calculated using the Rogan-Gladen-estimator and the confidence interval (CI) was set at 95% (Grenier and Gardner, Prev Vet Med 2000;45:43-59)

14 Latex agglutination test

15 524 bp DNA Ladder 100 bp + ve Control - ve Control Dog saliva Detection of rabies virus RNA in saliva specimen by RT-PCR The prevalence of rabies virus infection among stray dogs investigated was 0.03% (1 of 3,314)

16 Central Bangkok Bangkoks outskirts Central Bangkok Bangkoks outskirts No. tested No. positive Adjusted seroprevalence No. teested No. positive Adjusted seroprevalence % 95% CI % 95%CI 1, , Seroprevalence of rabies antibodies among stray dogs, based on an ELISA cut-off level of 0.5 EU/ml The overall seroprevalence of rabies antibody among stray dogs in Bangkok was 62% (95%CI 54-70%)

17 Map of Bangkok depicting the 50 districts. Shaded surface areas represent the districts in which the antibody seroprevalence among stray dogs reached 70%

18 Rabies infection is still prevalent in Bangkok Low vaccination coverage and ineffective management of stray dogs are the most likely reasons for the dog rabies elimination programs lack of success Conclusions

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