Presentation is loading. Please wait.

Presentation is loading. Please wait.

1 Molecular epidemiology of lyssaviruses in Eurasia Dr Lorraine McElhinney Veterinary Laboratories Agency (Weybridge), UK.

Similar presentations


Presentation on theme: "1 Molecular epidemiology of lyssaviruses in Eurasia Dr Lorraine McElhinney Veterinary Laboratories Agency (Weybridge), UK."— Presentation transcript:

1 1 Molecular epidemiology of lyssaviruses in Eurasia Dr Lorraine McElhinney Veterinary Laboratories Agency (Weybridge), UK

2 2 Previous Published Studies Russian RABV phylogeny (Kuzmin et al 2004) European RABV phylogeny (McElhinney et al 2006) Iranian RABV phylogeny (Nadin-Davis et al 2003) European RABV phylogeny (Bourhy et al 1999) Lyssavirus phylogeny (Badrane et al 2001) Middle East RABV phylogeny (David et al 2007)

3 3 Reported Rabies Cases Serbia & Montenegro Year No Total Wildlife Domestic Rabies present only in the Northern Regions of Serbia prior to 1990s (Sava, Danube) Rapid spread of rabies southwards at a rate of 20-50km/year since 1991

4 4 Places of origin of the isolates collected between (n=8) Foxes (n=7), Dog (n=1) SC =Somborcluster (5,10,11,12,16,18,19,23,25,26,27, 2831) ,6 =

5 5 Places of origin of the isolates collected between (n=33) Foxes (n=25), Cats (n=4), Dog, Deer, Cow, Badger (n=1)

6 6 Places of origin of the isolates collected between (n=9) Foxes (n=3), Dog (n=3), Cat, Horse and Cow (n=1) 2, 5 3,

7 7 Places of origin of the isolates collected between (n=31) Foxes (n=15), Cats (n=13), Dog (n=3)

8 8 Places of origin of the isolates collected between (n=66) Foxes (n=28), Cats (n=20), Dogs (n=10), Cows (n=4), Horses (n=2), Deer, Goat (n=1)

9 9 Isolate Distribution 147 virus isolates available for study Dates range between Varied Host species: 78 Foxes, 38 cats, 18 dogs, 13 others Additional published sequences obtained from Genbank Data Sources e.g. Kissi et al (1992), Bourhy et al (1999) Vanaga et al (2003), Nadin-Davis et al (2007) David et al (2007)

10 10 Rabies Virus Genome 400bp

11 11 Phylogenetic Tree (N400bp)

12 12 Isolates belonging to WE Group

13 13 Isolates belonging to EE Group

14 14 Isolates Closest to Middle East Group

15 15 Serbian Fox Group 100

16 16 Conclusions Evidence for a number of concurrent independent rabies cycles Supports previously published antigenic typing data (Stankov et al - mAbs) All FRY isolates within Cosmopolitan lineage Relationships - geographical, host species and chronological Potential for presence intermediate viruses (fox-dog- cat) Additional panel currently being analysed ( isolates) – assess survival of viral variants Future work will involve evolutionary clock analysis (Full N & G gene sequencing underway)

17 17 Conclusions (2) Royal Society Travel Grants – Russian & Chinese Programmes Sharing of isolates / collaborative studies Exchange visits Targeted surveillance programmes Russian Collaborative Programmes (VLA/Dr Botvinkin & Dr Kuzmin) publications: Kuzmin et al 2004, Botvinkin et al 2006, Mansfield et al 2006 Chinese Collaborative Programme (VLA/ Prof Tu & Dr Jiang) Canine RABV sequences analysed Active/passive targetted bat surveillance (Prof Tu to present data)

18 18 Prof A. Fooks, Dr L McElhinney D. Marston, Dr N Johnson, C Black Dr N Tordo (France) Prof Tu, Dr Jiang (China) Defra grant SE0420 Royal Society Dr S Stankov (Serbia & Montenegro) Dr T Muller (Germany) Acknowledgements


Download ppt "1 Molecular epidemiology of lyssaviruses in Eurasia Dr Lorraine McElhinney Veterinary Laboratories Agency (Weybridge), UK."

Similar presentations


Ads by Google