1. Ivermectin Effects on Caenorhabditis elegans (C.elegans)
Moyosore Olagoke

2. Background Information
1.Anti-parasitic Medication
Strongyloides stercoralis
Onchocerciasis volvulus
Pubic lice infestation
Scabies
Wuchereria bancrofti
2 Mechanism of action
Bind to GABA Channel
Hyperpolarization by ↑Cl Permeability
C95H146O28

3. Background Information
Phylum Nematoda
Feed on OP50 bacteria
Pseudocoelomates
Thrashing motion (contraction)
Female deposit 100,000 or more egg/day
Most individuals are Hermaphrodites
Life Cycle : >12hours
Caenorhabditis elegans

4. Research
Purpose :To determine the effect of Ivermectin on wild type (C.elegans)
Plan to extend strain
Complications
Method of introduction
Drug Test
Resistance strain

5. Ivermectin response to OP50 bacteria
Ivermectin has no effect on OP-50 bacteria
Avermectin is isolated from strepto Avermectins

6. Drug Introduction Ivermectin→ Agar Sample calculation
Stock g/1000цl in 100% DMSO
Stock 2- 10цl of stock 1 in ml 1%DMSO
15цl of stock 2 in 8цl Agar
10цg/цl x 15цl= 150цg
150 цg/8цl = 62.5цg/цl
Ivermectin→ Agar

7. Wild type C.elegans

8. Ivermectin: Wildtype worm
62.5цg/цl
Fewer numbers
No apparent movement
Worm appears transparent
18.75цg/цl
Same conclusion
Movement is perceived (~3 worms)

9. Comparison
Control
Ivermectin

10. Touch insensitive=Tapping

11. Future Analysis Growth Assay: Count the number of gravid adult
Reflects Pharyngeal function
Mechanosensation: Touch sensitivity
Neurological Response
Motility assay: Number of worm still thrashing
Locomotion

12. References Patient Drug Information From MedMaster. Maryland: 2007.
American Society of, Health-System Pharmacists Inc.
Patient Drug Information From MedMaster.
Maryland: 2007.
Jamison, Dean T., and Joel G. Breman.
Disease Control Priorities in Developing Countries. 2nd. New York: Oxford University Press, 1993.
John Keane and Leon Avery
Mechanosensory inputs influence Caenorhabditis elegans
pharyngeal activity via ivermectin sensitivity genes.
Genetics May; 164(1): 153–162. PMCID: PMC
Griffiths, Anthony J.F., and Jeffery H. Miller. HumanGenetics.
7th ed. New York: W.H. Freeman,1996.
Hart, Anne C. "Behavior." Worm Book
July April 2008
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capter.7133>.