1. ResistVir Mechanisms and sources of resistance Josef SPAK Academy of Sciences of the Czech Republic, Ceske Budejovice spak@umbr.cas.czspak@umbr.cas.cz http://www.umbr.cas.cz Institute of Plant Molecular Biology

2. Brassica viruses - TuMV, CaMV, TYMV, RaMV Fruit ilarviruses - ApMV, PDV, PNRSV Small fruit viruses –Strawberry - Nepoviruses, Aphid transmissible v. –Raspberry - Nepoviruses, RBDV –Currants - BRV, phytoplasma Experience and Interests

3. TuMV - EU Project „Disease management of turnip mosaic virus in oilseed rape“. Coordinator J.A. Walsh HRI, Wellesbourne, U.K. –Lehmann P, Petrzik K, Jenner C, Greenland A, Špak J, Kozubek E, Walsh JA.: Nucleotide and amino acid variation in the coat protein coding region of turnip mosaic virus isolates and possible involvement in the interaction with the brassica resistance gene TuRB01. Physiological and Molecular Plant Pathology 51: 195 - 208, 1997. –Tomimura K, Špak J, Katis N, Jenner CE, Walsh JA, Gibbs AJ, Ohshima K. Comparisons of the genetic structure of populations of Turnip mosaic virus in west and east Eurasia. Virology 330, 408-423, 2004. TYMV - seed transmission, field resistence in oilseed rape RaMV - variability of world isolates –Špak J, Kubelková D.: Serological variability among European isolates of radish mosaic virus. Plant Pathology 49: 295 - 301, 2000. –current work - full sequence, genome variability Brassica viruses

4. CP variability of ilarviruses Distribution of mutations on the CP genes PDV PNRSV ApMV 104/590 153/660 214/682 ApMV 203/675

5. Host-virus relationships Phylogeny of PNRSV Plum isolatesCherry isolates No correlation between host plant, CP sequence, geographical origin

6. Petrzik K. (2005) Capsid protein sequence gene analysis of Apple mosaic virus infecting pears Eur. J. Plant Pathol. 111 355- 360 Petrzik K., O.Lenz (2002) Remarkable variability of Apple mosaic virus capsid protein gene after the nucleotide position 141 Archives of Virology 147 1275-1285 Vašková D., K.Petrzik, J.Špak (2000) Molecular variability of the capsid protein of the prune dwarf virus Eur. J. Plant Pathol. 106 573-580 Vašková D., K.Petrzik, R.Karešová (2000) Variability and molecular typing of the woody-tree infecting necrotic ringspot ilarvirus Archives of Virology 145 699-709 Fruit ilarviruses - ApMV, PDV, PNRSV

7. Strawberry – Nepoviruses – SVBV – full sequence, genome variability – SVBV, SMYEV, SCV, SMoV  5th Framework project „Virus Detector“, coordinated by Wageningen UR, Netherlands Raspberry –Nepoviruses –RBDV Ribes – current work –Black currant reversion disease –Full blossom disease Small fruit viruses

8. THOMPSON, J.R., WETZEL, S., KLERKS, M.M., VASKOVÁ, D., SCHOEN, C.D., JELKMANN, W., SPAK, J.: Multiplex RT-PCR detection of four aphid-borne strawberry viruses in Fragaria spp. in combination with a plant mRNA specific internal control. - Journal of Virological Methods 111: 85-93, 2003. KLERKS MM, LINDNER JL, VASKOVA D, SPAK J, THOMPSON JR, JELKMANN W, SCHOEN CD. Detection and tentative grouping of Strawberry crinkle virus isolates. European Journal of Plant Pathology 110 (1): 45-52, 2004. VASKOVA D, SPAK J, KLERKS MM, SCHOEN CD, THOMPSON JR, JELKMANN W.: Real-time NASBA for detection of Strawberry vein banding virus. European Journal of Plant Pathology 110 (2): 213-221, 2004. Small fruit viruses