1. It’s all about ATP

2. How do you get it?

4. PMF

6. ATP synthesis using the PMF is ancient

7. Cytosolic enzymes convert glucose to pyruvate The ante is 2 ATP but you get 4 if you win Plus reducing power (that’s where it’s at) (and we’re not even in the mitochondrion yet)

8. In the cytosol

9. Still in the cytosol

10. OK, back to the mitochondrion

11. porin Cardiolipin, 76% protein

12. The BIG picture

13. Coupled to e- transport 2C +4C 6C Reducing power with carbon loss Free lunch 5C 4C Kreb Tricarboxylic Citric acid Cycle

14. Harnessing the reducing power Converting the redox potential into the PMF

17. 2 will get you 4: the Q cycle

18. Coupled to e- transport 5C 4C Remember this step?

19. matrix

20. Down to water

21. Now, drive ATP with the PMF