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Prodigiosin Synthesis UV-induced Colorless Mutants of Serratia marcescens.

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Presentation on theme: "Prodigiosin Synthesis UV-induced Colorless Mutants of Serratia marcescens."— Presentation transcript:

1 Prodigiosin Synthesis UV-induced Colorless Mutants of Serratia marcescens

2 amino acids a c b (g) d (ws) Prodigiosin enzyme x enzyme y enzyme z The Normal Pathway to Prodigiosin O NHNH NHNH N

3 A C b (g) d (ws) Prodigiosin enzyme x enzyme y enzyme z amino acids Serratia marcescens D1 (wild type) (DNA is not mutated for any of the genes)

4 a c B (g) D (ws) Prodigiosin enzyme x enzyme y enzyme z amino acids Serratia marcescens D1 (wild type) (DNA is not mutated for any of the genes)

5 a c b (g) d (ws) Prodigiosin enzyme x enzyme y enzyme z amino acids Serratia marcescens D1 (wild type) (DNA is not mutated for any of the genes)

6 amino acids a c b (g) d (ws) Prodigiosin enzyme x enzyme y enzyme z Serratia marcescens ( ) (DNA is mutated in enzyme x gene)

7 A C b (g) d (ws) Prodigiosin enzyme x enzyme y enzyme z amino acids Serratia marcescens ( ) (DNA is mutated in enzyme x gene)

8 c D (ws) Prodigiosin enzyme x enzyme y enzyme z amino acids b (g) A Serratia marcescens ( ) (DNA is mutated in enzyme x gene)

9 c D (ws) Prodigiosin enzyme x enzyme y enzyme z amino acids b (g) A Serratia marcescens ( ) (DNA is mutated in enzyme x gene) amino acids a c b (g) d (ws) Prodigiosin enzyme x enzyme y enzyme z Serratia marcescens ( ) (DNA is mutated in enzyme y gene)

10 c D (ws) Prodigiosin enzyme x enzyme y enzyme z amino acids b (g) A Serratia marcescens ( ) (DNA is mutated in enzyme x gene) amino acids b (g) d (ws) Prodigiosin enzyme x enzyme y enzyme z Serratia marcescens ( ) (DNA is mutated in enzyme y gene) A C

11 c D (ws) Prodigiosin enzyme x enzyme y enzyme z amino acids b (g) A Serratia marcescens ( ) (DNA is mutated in enzyme x gene) amino acids d (ws) Prodigiosin enzyme x enzyme y enzyme z Serratia marcescens ( ) (DNA is mutated in enzyme y gene) C a B (g)

12 c D (ws) Prodigiosin enzyme x enzyme y enzyme z amino acids b (g) A Serratia marcescens ( ) (DNA is mutated in enzyme x gene) amino acids d (ws) Prodigiosin enzyme x enzyme y enzyme z Serratia marcescens ( ) (DNA is mutated in enzyme y gene) C a B (g) 933WCF

13 933 c D (ws) Prodigiosin enzyme x enzyme y enzyme z amino acids b (g) A Serratia marcescens ( ) (DNA is mutated in enzyme x gene) amino acids d (ws) Prodigiosin enzyme x enzyme y enzyme z Serratia marcescens ( ) (DNA is mutated in enzyme y gene) C a B (g) 933WCF B (g) Prodigiosin B (g)

14 WCF 933 c D (ws) Prodigiosin enzyme x enzyme y enzyme z amino acids b (g) A Serratia marcescens ( ) (DNA is mutated in enzyme x gene) amino acids d (ws) Prodigiosin enzyme x enzyme y enzyme z Serratia marcescens ( ) (DNA is mutated in enzyme y gene) C a B (g) 933WCF D (ws) Prodigiosin D (ws)

15 a c b (g) d (ws) Prodigiosin enzyme x enzyme y enzyme z amino acids Serratia marcescens D1 (wild type) (DNA is not mutated for any of the genes) D1WCF d (ws) D1933 Why is D1 (so capable!) such a poor donor to either mutant… …compared to cross-feeding from either mutant? 933WCF 933WCF D (ws) B (g) b (g)


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