1. Viruses Chapter 19

2. A Borrowed Life Virus: an infections particle incapable of replicating outside of a cell, which consists of an RNA or DNA genome enclosed in a protein coat (______)  ________________ parasites Arguments for describing viruses as non-living  Can not ________ outside of host cell  Can not carry out ________________ outside of outside of host cell  Lack _________ and enzymes

3. Structure of Viruses  Capsids are made up of protein subunits called __________.  Viral envelopes: membranous envelope made up of phospholipids and membrane proteins that surrounds the capsids of some viruses

4. Virus Classification

5. Virus Reproduction 1. Virus enters host cell after host cell recognition 2. Host enzymes replicate virus DNA 3. Host enzymes transcribe viral DNA into viral mRNA, which is used to make capsid proteins 4. New virus particles assemble and leave host cell

6. Replication of Phages Lytic cycle: host cell ____ when releasing phages = cell death  Surface proteins  Restriction enzymes  CRISPR Lysogenic cycle: ________ of phage genome ≠ cell death  Prophage

7. Replication of Animal Viruses 1. Glycoproteins on viral envelope bind to receptors on host cell 2. Cellular enzymes digest capsid 3. Viral genome copied by viral RNA polymerase to make mRNA 4. Viral RNA functions as mRNA, which is translated to make capsid proteins and glycoproteins 5. Vesicles transport viral envelope to plasma membrane 6. Capsid assembles around viral genome 7. New virus buds from host cell 1 2 3 4 5 6 7

8. Replication of Retroviruses 1. Glycoproteins on viral envelope bind to ____________ on immune cell then fusing with plasma membrane and releasing capsid proteins and RNA into the host cell 2. ___________________ synthesizes DNA from viral RNA then subsequent DNA strands complementary to the first 3. Double stranded viral DNA incorporated into host cells DNA (_______) 4. Proviral genes transcribed to make RNA genome for progeny viruses and mRNA for translation into viral proteins 5. Capsids assembled around viral genomes 6. New viruses with viral envelope glycoproteins bud from host cell 1 2 3 4 5 6

9. Human Immunodeficiency Virus (HIV) Description:  ssRNA that is template for DNA synthesis  ________  Infects vital human immune system cells  Helper T cells, macrophages Treatment:  Azidothymidine (AZT)  Blocks _________________  Protease inhibitors  Blocks building of capsid

10. Ebola virus Description:  Class V: ssRNA that is template for mRNA synthesis  Infects human _______ system cells  Causes hemorrhagic fever  Small blood clots throughout body  Fever, vomiting, bleeding, organ failure Treatment:  No known treatment  Vaccines currently being tested

11. Influenza virus Description:  Class V: ssRNA that is template for mRNA synthesis  Infects epithelial cells of _________ system  H1N1 refers to surface proteins of virus  Hemagglutinin (H) and Neuraminidase (N) Treatment:  Rest and wait for immune response  Shortened and less severe when vaccinated  Antiviral drugs  Neuraminidase inhibitors block budding from host cell  M2 proton inhibitors block virus from entering host cell H1N1 influenza A virus Nucleoprotein (RNA) Lipid envelope Capsid Hemagglutinin Neuraminidase

12. Hepatitis Description:  At least five different unrelated viruses  Causes ______________ Hep A  ssRNA ________ envelope  Ingestion of contaminated food or water  Vaccination Hep C  ssRNA ____ envelope  Transmitted through contact with blood  Antiviral drugs

13. Poliomyelitis Description:  Class IV: ssRNA that can serve as mRNA  _% of infections target human nerve cells  Do not divide and cannot be replaced  Infected feces entering the mouth Treatment:  Few people show symptoms  Iron lung  __________ developed vaccine  No cure for polio

14. Vaccination 1. Antigen-Presenting Cell (APC) finds and ingests vaccine antigen then presents antigen on the cell surface. APCs travel to lymph nodes where other immune cells are congregated 2. T helper cells recognize the foreign antigen and alert other immune system cells 1 2

15. Vaccination 3. B-cells recognize the antigen and then divide into antigen specific Plasma B-cells which produce antibodies. Some B-cells become Memory B-cells 4. T cells recognize the foreign antigen and mature into Killer T-cells which find and destroy cells infected with the virus. Some T-cells become memory T helper cells and Memory Killer T-cells 4 3

16. Using Viruses  Gene transfer in gene therapy https://youtu.be/2pp17E4E-O8