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P28834Idh1 (isocitrate dehydrogenase 1 alpha-4-beta-4 subunit)8.997.8039.340 2822 P14843Aro3 (DAHP synthase)7.047.1041.540312921 P25294Sis1 (HSP40 family.

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Presentation on theme: "P28834Idh1 (isocitrate dehydrogenase 1 alpha-4-beta-4 subunit)8.997.8039.340 2822 P14843Aro3 (DAHP synthase)7.047.1041.540312921 P25294Sis1 (HSP40 family."— Presentation transcript:

1 P28834Idh1 (isocitrate dehydrogenase 1 alpha-4-beta-4 subunit)8.997.8039.340 2822 P14843Aro3 (DAHP synthase)7.047.1041.540312921 P25294Sis1 (HSP40 family chaperone)9.898.6019.132473820 P17505Mdh1 (mitochondrial malate dehydrogenase)8.507.1035.735243319 P06168Ilv5 (acetohydroxyacid reductoisomerase)9.106.9544.445232118 P50095Imd3 (IMP dehydrogenase homolog)7.047.3056.955263317 P07251Atp1 (mitochondrial F i F 0 -ATPase alpha subunit)9.107.2058.660173316 P15992Hsp26 (heat shock protein 26)5.315.2023.930484115 P40150Ssb2 (heat shock protein 70 isoforms)5.405.3066.649 (frg)92114 P00925Eno2 (enolase 2)5.70 46.921 (frg)141813 P00817Ipp1 (inorganic pyrophosphatase)5.365.2031.83433 12 P41277Gpp1 (DL–glycerol-3-phosphatase)6.106.0030.432342311 P32905Rps0a (40S ribosomal protein S0-A)4.704.9027.938192110 P22203Vma4 (Vacuolar ATPase Vi domain subunit E)5.305.1026.52820149 P10591Ssa1 (heat shock protein 70 isoforms)5.00 7027 (frg)19238 P06169Pdc1p (Pyruvate decarboxylase isozyme1)5.805.2061.631 (frg)19227 P07259Ura2p (Carbamyl phosphate synthase)5.605.3056.15214216 P15705Sti1p (stress induced protein)5.405.3066.3607235 P06633His7p (Imidazole glycerol phosphate synthase)5.30 61678234 Q05905YLR301w (Hypothetical ORF)5.10 27.52926303 P40106Gpp2 (DL-glycerol-3-phosphatase)5.30 27.93428302 P47143Ado1p (Putative adensoine kinase)5.00 36.44016191 SwissProt Accession No Top ranking protein in MS-Fit search d PredExpPredExp Matched peptide coverage (%) b No. of matching peptides (%) a Spot. No. Molecular mass (kDa) c pI c TABLE S1. Proteins induced by 300mM citric acid, pH 3.5, were identified by peptide mass fingerprinting 41

2 Table S1. (cont…) a The number of tryptic peptides assigned to the protein divided by the total number of tryptic pepides predicted for the protein expressed as a percentage b The number of amino acids in the matched peptides divided by the total number of amino acids in the predicted protein sequence expressed as a percentage c Theoretical PI and molecular weights were obtained from the protein entry in the MIPS database (http://mips.gsf.de/cgi-bin/proj/yeast) d The programme MS-Fit (URL: http://prospector.uscf.edu/ucsfhtml3.2/msfit.htm) was used to search the SwissProt database for proteins with calculated tryptic peptide masseshttp://prospector.uscf.edu/ucsfhtml3.2/msfit.htm that matched the measured experimentally-derived masses e The term frg in brackets indictaes that the protein detected was a proteolytic cleavage fragment 42

3 P38720Gnd1p (6-phosphogluconate dehydrogenase)6.194.853.925 (frg) c 273328 P38879Egd2 (GAL4 DNA-binding enhancer protein 2)4.80 18.7292712B P14540Fba1 (aldolase)5.516.0039.629 (frg) e 142126 Q04322Ydr533cp (hypothetical protein)5.266.0025.726562427 P28272Ura1 (dihydroorotate dehydrogenase)5.805.9034.835261725 Q46654Rps0b (40S ribosomal protein S0-B)4.694.9028323142A P32473Pdb1 (beta subunit of pyruvate dehydrogenase)5.235.0040.335342224 P29311Bmh1(Brain modulosignalin homolog)4.804.9030.131191623 SwissProt Accession No Top ranking protein in MS-Fit search d PredExpPredExp Matched peptide coverage (%) b No. of matching peptides (%) a Spot. No. Molecular mass (kDa) c PI c P00812Car1 (arginase)5.385.503641272629 TABLE S4. Proteins whose expression is affected by HOG1 identified by peptide mass fingerprinting 48 a The number of tryptic peptides assigned to the protein divided by the total number of tryptic pepides predicted for the protein expressed as a percentage b The number of amino acids in the matched peptides divided by the total number of amino acids in the predicted protein sequence expressed as a percentage c Theoretical PI and molecular weights were obtained from the protein entry in the MIPS database (http://mips.gsf.de/cgi-bin/proj/yeast) d The programme MS-Fit (URL: http://prospector.uscf.edu/ucsfhtml3.2/msfit.htm) was used to search the SwissProt database for proteins with calculated tryptic peptide masseshttp://prospector.uscf.edu/ucsfhtml3.2/msfit.htm that matched the measured experimentally-derived masses e The term frg in brackets indictaes that the protein detected was a proteolytic cleavage fragment


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