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P28834Idh1 (isocitrate dehydrogenase 1 alpha-4-beta-4 subunit)8.997.8039.340 2822 P14843Aro3 (DAHP synthase)7.047.1041.540312921 P25294Sis1 (HSP40 family.

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Presentation on theme: "P28834Idh1 (isocitrate dehydrogenase 1 alpha-4-beta-4 subunit)8.997.8039.340 2822 P14843Aro3 (DAHP synthase)7.047.1041.540312921 P25294Sis1 (HSP40 family."— Presentation transcript:

1 P28834Idh1 (isocitrate dehydrogenase 1 alpha-4-beta-4 subunit) P14843Aro3 (DAHP synthase) P25294Sis1 (HSP40 family chaperone) P17505Mdh1 (mitochondrial malate dehydrogenase) P06168Ilv5 (acetohydroxyacid reductoisomerase) P50095Imd3 (IMP dehydrogenase homolog) P07251Atp1 (mitochondrial F i F 0 -ATPase alpha subunit) P15992Hsp26 (heat shock protein 26) P40150Ssb2 (heat shock protein 70 isoforms) (frg)92114 P00925Eno2 (enolase 2) (frg) P00817Ipp1 (inorganic pyrophosphatase) P41277Gpp1 (DL–glycerol-3-phosphatase) P32905Rps0a (40S ribosomal protein S0-A) P22203Vma4 (Vacuolar ATPase Vi domain subunit E) P10591Ssa1 (heat shock protein 70 isoforms) (frg)19238 P06169Pdc1p (Pyruvate decarboxylase isozyme1) (frg)19227 P07259Ura2p (Carbamyl phosphate synthase) P15705Sti1p (stress induced protein) P06633His7p (Imidazole glycerol phosphate synthase) Q05905YLR301w (Hypothetical ORF) P40106Gpp2 (DL-glycerol-3-phosphatase) P47143Ado1p (Putative adensoine kinase) SwissProt Accession No Top ranking protein in MS-Fit search d PredExpPredExp Matched peptide coverage (%) b No. of matching peptides (%) a Spot. No. Molecular mass (kDa) c pI c TABLE S1. Proteins induced by 300mM citric acid, pH 3.5, were identified by peptide mass fingerprinting 41

2 Table S1. (cont…) a The number of tryptic peptides assigned to the protein divided by the total number of tryptic pepides predicted for the protein expressed as a percentage b The number of amino acids in the matched peptides divided by the total number of amino acids in the predicted protein sequence expressed as a percentage c Theoretical PI and molecular weights were obtained from the protein entry in the MIPS database (http://mips.gsf.de/cgi-bin/proj/yeast) d The programme MS-Fit (URL: was used to search the SwissProt database for proteins with calculated tryptic peptide masseshttp://prospector.uscf.edu/ucsfhtml3.2/msfit.htm that matched the measured experimentally-derived masses e The term frg in brackets indictaes that the protein detected was a proteolytic cleavage fragment 42

3 P38720Gnd1p (6-phosphogluconate dehydrogenase) (frg) c P38879Egd2 (GAL4 DNA-binding enhancer protein 2) B P14540Fba1 (aldolase) (frg) e Q04322Ydr533cp (hypothetical protein) P28272Ura1 (dihydroorotate dehydrogenase) Q46654Rps0b (40S ribosomal protein S0-B) A P32473Pdb1 (beta subunit of pyruvate dehydrogenase) P29311Bmh1(Brain modulosignalin homolog) SwissProt Accession No Top ranking protein in MS-Fit search d PredExpPredExp Matched peptide coverage (%) b No. of matching peptides (%) a Spot. No. Molecular mass (kDa) c PI c P00812Car1 (arginase) TABLE S4. Proteins whose expression is affected by HOG1 identified by peptide mass fingerprinting 48 a The number of tryptic peptides assigned to the protein divided by the total number of tryptic pepides predicted for the protein expressed as a percentage b The number of amino acids in the matched peptides divided by the total number of amino acids in the predicted protein sequence expressed as a percentage c Theoretical PI and molecular weights were obtained from the protein entry in the MIPS database (http://mips.gsf.de/cgi-bin/proj/yeast) d The programme MS-Fit (URL: was used to search the SwissProt database for proteins with calculated tryptic peptide masseshttp://prospector.uscf.edu/ucsfhtml3.2/msfit.htm that matched the measured experimentally-derived masses e The term frg in brackets indictaes that the protein detected was a proteolytic cleavage fragment


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