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NA-MIC National Alliance for Medical Image Computing Slicer3 Tutorial Manual Registration Dominik Meier, Ron Kikinis January 2010
National Alliance for Medical Image Computing © 2010, ARR Overview 1. Introduction 2. Prerequisites 3. Loading Example Dataset 10 sec 4. Creating New Transform 10 sec 5. Associate Transform with Image 5 sec 6. Move Image 2 min 7. Apply & Save 15 sec takes how long to do? Note: contains animated GIFs, view in Presentation Mode
National Alliance for Medical Image Computing © 2010, ARR Introduction / Background the two terms are often used synonymously. At a basic level they are the same thing. The goal is to bring two or more images into alignment such that corresponding content (anatomy, structure) appears in the same location an orientation when viewed together. In this tutorial example we have 2 MRI scans of the same subject, acquired with different head positions, so that when they are viewed simultaneously, they do not show matching anatomy. In other words they are shifted and rotated relative to eachother. Because the image is a digital representation, registration involves a reformatting of the stored image data. The common practice is to leave one image as is and reformat the other in the orientation of the first. You could think of it as a second re-scan of the subject, along the same orientation as the reference image. When you manually adjust the display of one image such that both images roughly show the same location, you are performing a type of registration. Because images are 3-dimensional, there are complex ways in which the two images can differ in orientation. For the brain example shown here we have relative shifts in any of 3 directions and 3 possible rotations (yaw, pitch & roll). This means as many as 6 parameters must be adjusted to register the two images. 3D Slicer offers both manual and automated ways to do this task. This tutorial explains the manual procedure. What is Image Registration or Data Fusion ? Before Registration After Registration animated GIFs: view in Presentation Mode
National Alliance for Medical Image Computing © 2010, ARR Prerequisites Slicer version 3.4 or later Example Dataset: download and extract the dataset for this tutorial: Slicer_ManualRegExample.zip. It should contain: ManualRegTutorial.pptPower Point File with this tutorial ManualRegTutorial.pdfPDF with this tutorial ManualRegTutorial_video1.movQuicktime Movie with Narrated Video Tutorial ManualRegistrationExample_SlicerScene.mrmlSlicer Scene File to load ManualRegistrationExample_fixed.nrrdfile of first MRI image ManualRegistrationExample_moving.nrrdfile of second MRI image to be aligned with first Tutorials to complete first (helpful but not required): –Slicer3Minute Tutorial –Loading and Viewing Data –http://www.slicer.org/slicerWiki/index.php/Slicer3.6:Traininghttp://www.slicer.org/slicerWiki/index.php/Slicer3.6:Training
National Alliance for Medical Image Computing © 2010, ARR 1. Loading Example Dataset To get the Example Dataset loaded into Slicer: 1. Menu: File: Load Scene… –Select the Slicer Scene file that comes with the downloaded example dataset, called: ManualRegistrationExample_SlicerScene.mrml –This will load all the associated images 2. Select Layout: From the icon bar, click on the Layout menu and select Four-up Layout. 3. Link Views: Click on the Ring Icon in any of the slice views to link all the views together. This will save you the work of making selections for each slice window separately. 4. Choose Foreground: MRI_moving 5. Choose Background: MRI_fixed.
National Alliance for Medical Image Computing © 2010, ARR Adjust Slice Views To interactively align images, we need to see them both at the same time: Set Visibility Slider to halfway between foreground and background. This allows you to see both images. Note how the display changes as you move the slider. Note: For your own dataset, you may need to adjust the level as well as each images Window & Level settings to comfortably see both images.
National Alliance for Medical Image Computing © 2010, ARR Create New (blank) Transform 1. You can create a new Transform in the Data module directly: 2. Right-click on the Scene node in the MRML tree. From the pulldown menu select: Insert Transform Node 3. We change the name of the new transform: 1. Click on the little triangle to expand the MRML Node Inspector tab at the bottom of the panel 2. select the new node (vtkMRMLLinearTransformNode) 3. type the new name, e.g. ManualInit 4. hit ENTER
National Alliance for Medical Image Computing © 2010, ARR Link Image to New Transform 1. You see the newly created transform listed along with the two image volumes. We now associate the moving image with the transform: 2. In the MRML tree window, select the node for the MRI_moving image and drag it on top of the Manual Init transform. You should now see the diagram updated with the volume listed within the transform animated GIF: view in Presentation Mode
National Alliance for Medical Image Computing © 2010, ARR Move Image 1. Return To the Transforms module 2. Move the sliders to align the two images: 1. Move the translation sliders labeled IS to the left until it reads about -21. Watch the display update as you move the slider 2. Move the PA translation slider to the left while watching the display until the two brain roughly align. The PA slider should read around Move the IS rotation slider to rotate the volume clockwise until the axial planes have roughly the same orientation. (ca. 14 degrees). 4. Move the LR translation slider to the right to compensate for the shift that occurred from the rotation. Reading now is ca If you get stuck you can easily start over by clicking the Identity button at the bottom, which will reset the transform. 6. You can tune the sensitivity of the translation sliders by setting the min and max. Setting smaller range allows more precise changes. 7. You can also move by entering the numbers in the text fields and the hit the TAB or Return key. animated GIF: view in Presentation Mode
National Alliance for Medical Image Computing © 2010, ARR Apply Transform You can now use this transform as input to an automated registration: In most automated registration modules you will find a menu called Load Transform or Initial Transform. To use simply select from the pulldown menu. You can save the transform to apply elsewhere. This is done via the File/Save menu. You can apply the transform to other volumes. You can drag any other volume inside the transform to apply it and then select Harden Transform (see next page) to finalize, or you can use the Resample Volume module for more options.
National Alliance for Medical Image Computing © 2010, ARR Apply & Save We apply the transform to the moving image and save the result volume: 1. Go back to the Data module 2. Right-click on the MRI_moving image. From the pulldown menu, select Harden Transform. This finalizes the transform and resamples the volume in the new orientation. You will see the MRI_moving node moved back out into the main hierarchy level.
National Alliance for Medical Image Computing © 2010, ARR Save 1. Select Save from the File Menu. In the Dialog uncheck all lines except for MRI_moving. In the FileName field, double click and select a new filename, e.g. ManualResult.nrrd 2. To also save the transform, check the box next to Manual Init. The transorm will be saved as a small text file containing all the necessary parameters. 3. click Save.
National Alliance for Medical Image Computing © 2010, ARR What Next Try the automated Registration Tutorial or one of the tutorials from the Registration Case Library. –http://na- mic.org/Wiki/index.php/Projects:RegistrationDocumentation:UseCaseIn ventoryhttp://na- mic.org/Wiki/index.php/Projects:RegistrationDocumentation:UseCaseIn ventory –http://www.slicer.org/slicerWiki/index.php/Slicer3:Registration#Registrat ion_in_3D_Slicer|Mainhttp://www.slicer.org/slicerWiki/index.php/Slicer3:Registration#Registrat ion_in_3D_Slicer|Main Feedback: anything amiss? If you have suggestions on how we can improve this and other documentation, please let us know: visit: –http://na-mic.org/Wiki/index.php/Projects:RegistrationDocumentationhttp://na-mic.org/Wiki/index.php/Projects:RegistrationDocumentation
National Alliance for Medical Image Computing © 2010, ARR Acknowledgements National Alliance for Medical Image Computing NIH U54EB Neuroimage Analysis Center NIH P41RR S1 (ARRA Suppl)
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