Presentation on theme: "Salmonid Pathogens in the Columbia Cascade Province Frank Loge 1 Douglas Call 2 Michael Barber 1 1 Department of Civil and Environmental Engineering 2."— Presentation transcript:
Salmonid Pathogens in the Columbia Cascade Province Frank Loge 1 Douglas Call 2 Michael Barber 1 1 Department of Civil and Environmental Engineering 2 Department of Veterinary Microbiology & Pathology and WSU/UofI Center for Reproductive Biology Washington State University
Hypotheses 1.Source affects pathogen load 2.Anthropogenic pollution affects in-stream survival and propagation of pathogens 3.Pathogen concentrations vary with light, temperature and aquatic habitat
Rationale 14 salmonid species are listed as either threatened or threatened. Disease impacts every stage of salmonid life-history. Very little information available on ecology of infectious disease agents relative to fish populations, watersheds or habitats.
Significance Factors that contribute to large-scale salmonid infections are unknown and represent a critical data gap (Upper Middle Mainstem Subbasin report) Consistent with Northwest Power Planning Council Fish and Wildlife Program Consistent with NMFS and USFWS 2000 Biological Opinion and Action Agencies Implementation Plan (actions 141, 184, 11.2, )
Objectives Develop a microarray detector and sampling protocol for multiplex detection of salmonid pathogens Characterize the spatial and temporal occurrence of pathogens and water quality parameters Identify possible sources of salmonid pathogens and correlate with anthropogenic pollution
Microarrays permit efficient multiplex analysis with multiple internal controls CP1CP2R.s.A.s.Y.r.A.s.Y.r.R.s. p57Fp57RProbe AProbe B 16S rDNA p57 gene Why use microarrays?
Detecting BKD (Renibacterium salmoninarum)
Overview of monitoring protocol
Expected Results and Benefits Provide a field deployable monitoring protocol and a risk-based methodology for interpreting results Provide critical information on the temporal and spatial occurrence of planktonic salmonid pathogens Identify possible sources, fate, and transport of salmonid pathogens and relationships with specific water quality characteristics