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Project 200007900: Assess Resident Fish Stocks of the Owyhee/Bruneau Basin on the Duck Valley Indian Reservation Mattie Allen, Shoshone Paiute Tribes.

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Presentation on theme: "Project 200007900: Assess Resident Fish Stocks of the Owyhee/Bruneau Basin on the Duck Valley Indian Reservation Mattie Allen, Shoshone Paiute Tribes."— Presentation transcript:

1 Project 200007900: Assess Resident Fish Stocks of the Owyhee/Bruneau Basin on the Duck Valley Indian Reservation Mattie Allen, Shoshone Paiute Tribes

2 Duck Valley Indian Reservation

3

4 Coastal Rainbow Trout and Redband Trout Distributions

5 Redband Trout Distribution and Status in the Owyhee Subbasin

6 Past Activities in the East Fork Owyhee River Basin Grazing PracticesHatchery Releases Hatchery coastal rainbow trout stocked over a period of several decades Riparian corridors remained unprotected despite heavy grazing pressure

7 Goal: Preserve native redband trout populations in East Fork Owyhee River and its tributaries.

8 Objectives Evaluate the genetic variation within and among putative native redband trout populations from 14 locations in the upper East Fork Owyhee River watershed Evaluate whether F x progeny from events of introgression between hatchery rainbow trout and native redband trout or subsequent introgressed generations are present in 14 locations in the upper East Fork Owyhee River watershed

9 Location of Upper East Fork Owyhee River Collection Sites

10 Genetic Analyses mtDNA Gene regions: CytB and ND2 Restriction enzymes: Ava I, Dde I, Hae III Dpn II, Hha I, Hinf I, Mse I, Msp I, Rsa I, Taq I, Microsatellites Primers: Omy 7 Omy 8 Omy 10 Omy 34 OMM 1020

11 Composite Mitochondrial Haplotype Frequencies for Putative Redband Trout Populations from Upper East Fork Owyhee River Population I II IIIIVVVI Fawn Creek, Upper (N=60)0.650.350000 Fawn Creek, Lower (N=60)0.550.3330.050.033 0 Highline Canal (N=15)0.6670.3330000 Jones Creek (N=27)0.7040.0370.1110.0740.037 Miller Creek (N=44)0.5680.2730.0680.09100 Reed Creek (N=14)0.6430.3570000 Sheep Creek (N=52)0.4620.3850.0770.05800.019 Skull Creek, Upper (N=27)0.6670.2590.074000 Skull Creek, Lower (N=39)0.6410.2560.103000 Skull Creek, N.F. Lower (N=42) 0.6190.3570.024000 Spring Creek (N=60)0.60.3170.017 0.050 Summit Creek (N=46)0.4780.2610.1520.0220.0870 Composite Mitochondrial Haplotypes

12 IIIIIIIVV II0.620 III1.0801.160 IV2.1301.7701.590 V1.5601.9001.7701.320 VI1.1601.5401.9000.7601.090 Percentage Sequence Divergence Among Observed mtDNA Haplotypes from the Upper East Fork Owyhee River

13 01.02.0 II I III IV VI V Inferred Evolutionary Relationships Among mtDNA Haplotypes in the Upper East Fork Owyhee River Percent Sequence Divergence

14 Composite Mitochondrial Haplotype Frequencies for Putative Redband Trout Populations from Upper East Fork Owyhee River Population I II IIIIVVVI Fawn Creek, Upper (N=60)0.650.350000 Fawn Creek, Lower (N=60)0.550.3330.050.033 0 Highline Canal (N=15)0.6670.3330000 Jones Creek (N=27)0.7040.0370.1110.0740.037 Miller Creek (N=44)0.5680.2730.0680.09100 Reed Creek (N=14)0.6430.3570000 Sheep Creek (N=52)0.4620.3850.0770.05800.019 Skull Creek, Upper (N=27)0.6670.2590.074000 Skull Creek, Lower (N=39)0.6410.2560.103000 Skull Creek, N.F. Lower (N=42) 0.6190.3570.024000 Spring Creek (N=60)0.60.3170.017 0.050 Summit Creek (N=46)0.4780.2610.1520.0220.0870 Composite Mitochondrial Haplotypes

15 Management Implications and Emerging Issues ?


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