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Project 200007900: Assess Resident Fish Stocks of the Owyhee/Bruneau Basin on the Duck Valley Indian Reservation Mattie Allen, Shoshone Paiute Tribes.

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Presentation on theme: "Project 200007900: Assess Resident Fish Stocks of the Owyhee/Bruneau Basin on the Duck Valley Indian Reservation Mattie Allen, Shoshone Paiute Tribes."— Presentation transcript:

1 Project : Assess Resident Fish Stocks of the Owyhee/Bruneau Basin on the Duck Valley Indian Reservation Mattie Allen, Shoshone Paiute Tribes

2 Duck Valley Indian Reservation

3

4 Coastal Rainbow Trout and Redband Trout Distributions

5 Redband Trout Distribution and Status in the Owyhee Subbasin

6 Past Activities in the East Fork Owyhee River Basin Grazing PracticesHatchery Releases Hatchery coastal rainbow trout stocked over a period of several decades Riparian corridors remained unprotected despite heavy grazing pressure

7 Goal: Preserve native redband trout populations in East Fork Owyhee River and its tributaries.

8 Objectives Evaluate the genetic variation within and among putative native redband trout populations from 14 locations in the upper East Fork Owyhee River watershed Evaluate whether F x progeny from events of introgression between hatchery rainbow trout and native redband trout or subsequent introgressed generations are present in 14 locations in the upper East Fork Owyhee River watershed

9 Location of Upper East Fork Owyhee River Collection Sites

10 Genetic Analyses mtDNA Gene regions: CytB and ND2 Restriction enzymes: Ava I, Dde I, Hae III Dpn II, Hha I, Hinf I, Mse I, Msp I, Rsa I, Taq I, Microsatellites Primers: Omy 7 Omy 8 Omy 10 Omy 34 OMM 1020

11 Composite Mitochondrial Haplotype Frequencies for Putative Redband Trout Populations from Upper East Fork Owyhee River Population I II IIIIVVVI Fawn Creek, Upper (N=60) Fawn Creek, Lower (N=60) Highline Canal (N=15) Jones Creek (N=27) Miller Creek (N=44) Reed Creek (N=14) Sheep Creek (N=52) Skull Creek, Upper (N=27) Skull Creek, Lower (N=39) Skull Creek, N.F. Lower (N=42) Spring Creek (N=60) Summit Creek (N=46) Composite Mitochondrial Haplotypes

12 IIIIIIIVV II0.620 III IV V VI Percentage Sequence Divergence Among Observed mtDNA Haplotypes from the Upper East Fork Owyhee River

13 II I III IV VI V Inferred Evolutionary Relationships Among mtDNA Haplotypes in the Upper East Fork Owyhee River Percent Sequence Divergence

14 Composite Mitochondrial Haplotype Frequencies for Putative Redband Trout Populations from Upper East Fork Owyhee River Population I II IIIIVVVI Fawn Creek, Upper (N=60) Fawn Creek, Lower (N=60) Highline Canal (N=15) Jones Creek (N=27) Miller Creek (N=44) Reed Creek (N=14) Sheep Creek (N=52) Skull Creek, Upper (N=27) Skull Creek, Lower (N=39) Skull Creek, N.F. Lower (N=42) Spring Creek (N=60) Summit Creek (N=46) Composite Mitochondrial Haplotypes

15 Management Implications and Emerging Issues ?


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