1. Manchester Spring/Summer Chinook Captive Broodstock Project 199606700 2003-2005 Accomplishments Resource Enhancement and Utilization Technologies Division Manchester Research Station Northwest Fisheries Science Center National Marine Fisheries Service National Oceanic and Atmospheric Administration 2725 Montlake Boulevard E. Seattle, Washington 98112 Desmond Maynard, Carlin McAuley, & Thomas Flagg

2. Rationale and Significance to Regional Programs Salmon Subbasin Summary Grande Ronde Subbasin Summary Mainstem/Systemwide Artificial Propagation Summary RPA 175, 176, & 177 of 2000 NMFS BiOP 1995 NMFS Snake River Salmon Recovery Plan

3. Relationship to Other Projects Idahos Salmon River 199700100 Captive Rearing Project for Salmon River Chinook Salmon (IDFG) 198909803 Salmon Supplementation Studies in Idaho (SBT) Oregons Grande Ronde River 199801001 Grande Ronde Basin Spring Chinook Captive Brood Program O&M (ODFW) 199801006 Captive Broodstock Artificial Propagation Project (NPT) 199800703 Grande Ronde Supplementation O&M/M&E (CTUIR) Systemwide 199305600 An Assessment of the Status of Captive Broodstock Technology for Pacific Salmon (NOAA) Interagency activities coordinated through CSCPTOC & CCBPTOT

4. Snake River Spring/Summer Chinook Salmon Captive Broodstock Projects Collect 0-age parr from tributaries Reared in freshwater at Lookingglass/Wallowa Fish Hatcheries 33-50% of smolts transferred to Manchester for seawater rearing Reared in seawater at Manchester until they display first signs of maturation Maturing adults transferred to Bonneville Freshwater Captive Broodstock Facility Mature adults spawned at Bonneville Captive Broodstock Facility Progeny reared at Lookingglass Hatchery Progeny released as smolts from tributary acclimation sites Collect eyed eggs from redds Reared in freshwater at Eagle Hatchery 100% of smolts transferred to Manchester for seawater rearing Reared in seawater at Manchester until they display the first signs of maturation Maturing adults transferred to Eagle Freshwater Hatchery Mature adults released into streams and allowed to spawn naturally Progeny rear naturally in the tributaries their parents spawned in Grande Ronde RiverSalmon River

5. 2003-2005 Objectives Conduct marine rearing of endangered Snake River spring/summer chinook salmon maintained in Regional captive broodstock programs Upgrade seawater delivery system

6. Manchester Research Station The Regions Facility for Marine Rearing of ESA Listed Anadromous Salmonids

7. ESA Captive Broodstock Seawater Rearing Facilities

8. Seawater Delivery System Two 50 HP pumps supply 1,100 gpm of seawater 700-m pipeline Backup pump and emergency generators

9. Seawater Processing Sand Filters remove all particles larger than 20 microns Cartridge Filter remove all particles larger than 5 microns Ultra violet light used to disinfect filtered water Water can be chilled several degrees below ambient Aeration tower ensures oxygen level returned to saturation value

10. Ozone Sterilization of Effluent Protects Puget Sound Permits Columbia Basin stocks to be transferred into the facility for seawater rearing

11. Smolt Transfer ODFW sentinels are sent to MRS first to ensure the fish are physiologically ready for seawater rearing Gently transitioned to seawater Initially reared in Building 12

12. Marine Rearing Reared 0.3-3 years Minimal disturbance and handling Dry commercial diet Automatic Feeders Maintained on natural photoperiod Ramped lighting to prevent startle 0.84 kg/LPM maximum loading density 15 kg/m 3 maximum rearing density

13. Smolt to Prespawning Adult Survival (Mean + SE) of Broodyear 1994 through 2000 Snake River Spring/Summer Chinook Salmon During Seawater Rearing

14. Length and Weight Sampling

15. Length in millimeters (Mean + SE) of Broodyear 1994 through 2002 Prespawning Snake River Spring/Summer Chinook Salmon Adults Just Prior to Transfer Back to Freshwater

16. Weight in grams (Mean + SE) of Broodyear 1994 through 2002 Prespawning Snake River Spring/Summer Chinook Salmon Adults Just Prior to Transfer Back to Freshwater

17. Maturation Sort And Transfer to Freshwater

18. Percentage (Mean + SE) of Broodyear 1994 through 2000 Idaho and Oregon Stocks Reared in Seawater Maturing as Precocious, Jack, and Adult (4-6 Year Old) Fish

19. NMFS Snake River Chinook Salmon Production Brood YearNumber 1994358 1995246 1996368 1997548 1998728 1999713 2000837 2001743 2002504 2003210 TOTAL 5,265

20. Seawater Pipeline Replacement Conceptual Design NEPA JARPA Contract –Pipeline –Support Pier Construct –Pipeline –Support Pier

21. Construction 1

22. Construction 2

23. Construction 3

24. Questions