1. 2002 7WCGALP (HDN-1) Performance of Holstein clones in the United States H.D. NORMAN,*,1 T.J. LAWLOR, 2 and J.R. WRIGHT 1 1 Animal Improvement Programs Laboratory Agricultural Research Service, USDA Beltsville, MD 20705-2350, USA 2 Holstein Association USA, Brattleboro, VT 05302, USA *dnorman@aipl.arsusda.gov 301-504-8334 Photo courtesy Infigen

2. 2002 7WCGALP (HDN-2) National Research Council (USA) Subcommittee to identify and to prioritize science-based risks of genetically modified animals Report will decide regulation of cloned animals by U.S. Food and Drug Administration

3. 2002 7WCGALP (HDN-3) Cloning technology Embryo splitting  First U.S. registered Holstein calves: 1982  Identical nuclear and mitochondrial DNA Nuclear transfer  First U.S. registered Holstein calves: 1989  Nearly identical nuclear DNA, but different mitochondrial DNA  Almost no recording of recipient cell identity  Full siblings versus identical animals

4. 2002 7WCGALP (HDN-4) Objectives Document performance of clones:  Yield: milk, fat, protein  Fitness: somatic cell score (SCS), productive life (PL) Examine performance differences between embryo-split and nuclear- transfer clones

5. 2002 7WCGALP (HDN-5) Data Holstein Association USA registrations for embryo- split and nuclear-transfer clones  Gender  Birth year Pedigree merit  Mean of parent predicted transmitting abilities (PTAs)  Clone type (embryo split vs. nuclear transfer)  All U.S. Holsteins born in same year and milk recorded through Dairy Herd Improvement

6. 2002 7WCGALP (HDN-6) Embryo-split clone population

7. 2002 7WCGALP (HDN-7) Nuclear-transfer clone population

8. 2002 7WCGALP (HDN-8) Female pedigree merit: Milk Birth year Mean of parent PTAs (kg) Embryo splitNuclear transferPopulation 1983-487...-645 1985-283...-554 1987-322...-459 1989-211-113-344 1991-33+46-233 1993+88-29-114 1995+146...0 1997+342...+122 1999+432+645+206

9. 2002 7WCGALP (HDN-9) Female pedigree merit: Fat Birth year Mean of parent PTAs (kg) Embryo splitNuclear transferPopulation 1983-15...-22 1985-10...-19 1987-9...-15 1989-2-3-10 1991+1+3-7 1993+4 -4 1995+6...0 1997+10...+4 1999+12 +6

10. 2002 7WCGALP (HDN-10) Female pedigree merit: Protein Birth year Mean of parent PTAs (kg) Embryo splitNuclear transferPopulation 1983-13...-18 1985-10...-16 1987-10...-14 1989-5-2-10 19910+2-7 1993+5+1-3 1995+5...0 1997+13...+4 1999+15+23+7

11. 2002 7WCGALP (HDN-11) Mean standardized traits Embryo splittingNuclear transfer Trait ClonesFull sibs ClonesFull sibs Milk (kg)10,57710,85010,19710,052 Fat (kg)386394367368 Fat (%)3.663.623.613.66 Protein (kg)315324317306 Protein (%)2.98 3.123.03 SCS3.03.13.73.1 PL (mo)25.625.321.430.3

12. 2002 7WCGALP (HDN-12) Deviation from contemporaries Embryo splittingNuclear transfer Trait ClonesFull sibs ClonesFull sibs Milk (kg)-273-93-525-519 Fat (kg)-5-24-15 Protein (kg)-50-8-11

13. 2002 7WCGALP (HDN-13) Mean PTA Embryo splittingNuclear transfer Trait Clone s Full sibs Clone s Full sibs Milk (kg)-67-34-105-63 Fat (kg)00-50 Fat (%)0.02 -0.010.02 Protein (kg)0000 Protein (%)0.01 0.030.02 SCS3.09 3.163.14 PL (mo)0.10.2-0.4-0.1

14. 2002 7WCGALP (HDN-14) Famous split-embryo bulls Bull Daugh- ters (no.) PTA Milk (kg) Fat (kg) Pro- tein (kg) Produc- tive life (mo) Somatic cell score Duplicate477+156-19-7-0.83.28 Divide606+143-18-7-0.73.26 Wind Kaddy5032+347+13+10-1.13.55 SW Kaddy506+261+14+8-1.13.48

15. 2002 7WCGALP (HDN-15) Decreased number of embryo-split males and all nuclear-transfer clones Animals selected for cloning slightly superior genetically to population for yield traits Yields similar for nuclear-transfer clones and their non-cloned full siblings Yields of embryo-split clones slightly less than yields of their non-cloned full siblings Conclusions