1. Microscopes: Types, parts, magnification, use!
Do you see what I see?

2. General Parts
The following parts can be found (in some shape) on ALL types of microscopes.
The example diagram is a Compound Light Microscope and is the type we will be using in class.

3. General Parts

4. Dissecting Microscope

5. Dissecting Microscope
Light is reflected off a specimen into two different objectives and eyepieces (or oculars.)
Provides a 3D view of the specimen.
Usually shows surface features of the specimen.
Useful from 60X to 80X magnification.

6. Dissecting Microscopes

7. Compound Light Microscopes

8. Compound Light Microscopes
Light shines through the specimen and into a single objective lens and then through the eyepiece (ocular).
Provides a 2D view.
Specimen must be thin and the light must be able to pass through the slide.
Useful up to about 1000X magnification.

9. Compound Light Microscopes

10. Scanning Electron Microscopes (SEM)

11. SEM’s Uses a beam of electrons instead of light.
The beam of electrons is passed over the specimen and the electrons scatter. These scattered electrons are detected and processed by computer to form an image on a screen.
Useful up to 10000X magnification.

12. SEM’s

13. Tunneling Electron Microscopes (STM)

14. STM’s Uses a beam of electrons instead of light.
Uses probes and electrons to determine the difference in voltage as the probe passes over the specimen.
Can view objects as small as an atom.

15. STM’s

16. Magnification
Magnification is the ability to make objects look larger.
EX: a magnification of 200X means that the object looks 200 times larger then life.
Magnification bar is a line of a certain length that is included with an image to show the scale.

17. Magnification
Calculating magnification is as simple as multiplying the given numbers.
Each objective is labeled with its power and the ocular is labeled with its power so that the total magnification equals the objective times the ocular.
EX: the objective is labeled 30X and the ocular is labeled 10X so the total magnification is?

18. Getting to know your microscope
We will now get to know our microscopes. Be prepared to start your pre-lab tomorrow! That means you need to have your bound lab notebook!